Targeting the hepatitis B virus precore antigen with a novel IgNAR single variable domain intrabody.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Virology

• Publication date

  2011-03-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.virol.2010.12.034PMID 21239030
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Serum hepatitis B surface antigen and hepatitis B e antigen titers: Disease phase influences correlation with viral load and intrahepatic hepatitis B virus markers

  2010cited by this paper
• Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies.

  2010cited by this paper
• Bioinformatic analysis of the hepadnavirus e‐antigen and its precursor identifies remarkable sequence conservation in all orthohepadnaviruses

  2010influential reference
• Characterization of Genotype-Specific Carboxyl-Terminal Cleavage Sites of Hepatitis B Virus e Antigen Precursor and Identification of Furin as the Candidate Enzyme

  2009cited by this paper
• Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

  2009cited by this paper
• Llama‐derived single‐domain intrabodies inhibit secretion of hepatitis B virions in mice

  2009cited by this paper
• HBeAg and hepatitis B virus DNA as outcome predictors during therapy with peginterferon alfa‐2a for HBeAg‐positive chronic hepatitis B

  2008cited by this paper
• A valine to phenylalanine mutation in the precore region of hepatitis B virus causes intracellular retention and impaired secretion of HBe‐antigen

  2008cited by this paper
• An scFv intrabody against the nonamyloid component of alpha-synuclein reduces intracellular aggregation and toxicity.

  2008cited by this paper
• Display scaffolds: protein engineering for novel therapeutics.

  2008cited by this paper
• Regulation of Toll‐like receptor‐2 expression in chronic hepatitis B by the precore protein

  2007influential reference
• Maturation of shark single-domain (IgNAR) antibodies: evidence for induced-fit binding.

  2007cited by this paper
• Reduced Expression of Toll-Like Receptor 2 on Peripheral Monocytes in Patients with Chronic Hepatitis B

  2006cited by this paper
• In vitro improvement of a shark IgNAR antibody by Qbeta replicase mutation and ribosome display mimics in vivo affinity maturation.

  2006cited by this paper
• First molecular and biochemical analysis of in vivo affinity maturation in an ectothermic vertebrate.

  2006cited by this paper
• Antibody repertoire development in cartilaginous fish.

  2006cited by this paper
• Immune Tolerance Split between Hepatitis B Virus Precore and Core Proteins

  2005cited by this paper
• Trapping Prion Protein in the Endoplasmic Reticulum Impairs PrPC Maturation and Prevents PrPSc Accumulation*

  2005cited by this paper
• Structure, assembly, and antigenicity of hepatitis B virus capsid proteins.

  2005cited by this paper
• Cellular response to conditional expression of the hepatitis B virus precore and core proteins in cultured hepatoma (Huh-7) cells.

  2005cited by this paper
• Structure of a shark IgNAR antibody variable domain and modeling of an early‐developmental isotype

  2005influential reference
• Selection and affinity maturation of IgNAR variable domains targeting Plasmodium falciparum AMA1

  2004influential reference
• A function of the hepatitis B virus precore protein is to regulate the immune response to the core antigen.

  2004cited by this paper
• Crystal Structure of a Shark Single-Domain Antibody V Region in Complex with Lysozyme

  2004influential reference
• Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures

  2004cited by this paper
• Structural evidence for evolution of shark Ig new antigen receptor variable domain antibodies from a cell-surface receptor.

  2004influential reference
• Effect of the G1896A precore mutation on drug sensitivity and replication yield of lamivudine‐resistant HBV in vitro

  2003cited by this paper
• Inhibition of Tat-mediated Transactivation and HIV-1 Replication by Human Anti-hCyclinT1 Intrabodies*

  2003cited by this paper
• Endocytosis of Hepatitis B Immune Globulin into Hepatocytes Inhibits the Secretion of Hepatitis B Virus Surface Antigen and Virions

  2003cited by this paper
• Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70.

  2003influential reference
• Proteolytic Processing of the Hepatitis B Virus e Antigen Precursor

  2003cited by this paper
• Isolation of the new antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries.

  2001influential reference
• Bacteriophage Library Construction and Selection of Recombinant Antibodies

  1999cited by this paper
• Unique single‐domain antigen binding fragments derived from naturally occurring camel heavy‐chain antibodies

  1999cited by this paper
• Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus

  1998cited by this paper
• The secreted hepatitis B precore antigen can modulate the immune response to the nucleocapsid: a mechanism for persistence.

  1998cited by this paper
• Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins.

  1998cited by this paper
• Use of mutator cells as a means for increasing production levels of a recombinant antibody directed against Hepatitis B.

  1997cited by this paper
• Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication

  1997influential reference
• Global status of hepatitis B immunisation.

  1996cited by this paper
• Engineering antibody Fv fragments for cancer detection and therapy: Bisulfide-stabilized Fv fragments

  1996cited by this paper
• Analysis of the precore DNA sequence and detection of precore antigen in liver specimens from patients with anti‐hepatitis b e—positive chronic hepatitis

  1995cited by this paper
• A new antigen receptor gene family that undergoes rearrangement and extensive somatic diversification in sharks

  1995cited by this paper
• Anatomy of the antibody molecule.

  1994cited by this paper
• Current protocols in immunology

  1991cited by this paper
• Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors.

  1991influential reference
• Is a function of the secreted hepatitis B e antigen to induce immunologic tolerance in utero?

  1990cited by this paper
• Conformations of immunoglobulin hypervariable regions

  1989cited by this paper
• Expression of the precore region of an avian hepatitis B virus is not required for viral replication

  1987cited by this paper
• Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.

  1987influential reference
• Secretion of beta-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change.

  1986cited by this paper
• Immunochemical structure of hepatitis B e antigen in the serum.

  1983cited by this paper
• Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.

  1982influential reference

• VNAR: shark single-domain antibodies for the new era of medical biotechnology

  2026cites this paper
• HBV p22-interacting protein C1QBP inhibits viral replication through impeding nucleocapsid formation and nuclear import

  2025cites this paper
• The in vitro replication phenotype of hepatitis B virus (HBV) splice variants Sp3 and Sp9 and their impact on wild-type HBV replication

  2024cites this paper
• CRISPR-Cas13b-mediated suppression of hepatitis B virus replication and protein expression.

  2024cites this paper
• Exploring shark VNAR antibody against infectious diseases using phage display technology.

  2023cites this paper
• Shark IgNAR: The Next Broad Application Antibody in Clinical Diagnoses and Tumor Therapies?

  2023cites this paper
• VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)

  2023cites this paper
• Screening and Characterization of Shark-Derived VNARs against SARS-CoV-2 Spike RBD Protein

  2022cites this paper
• The in vitro replication phenotype of hepatitis B virus (HBV) splice variant Sp1.

  2022cites this paper
• Constrained evolution of overlapping genes in viral host adaptation: Acquisition of glycosylation motifs in hepadnaviral precore/core genes

  2022cites this paper
• Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development.

  2022cites this paper
• A broad-spectrum nanobody targeting the C-terminus of the hepatitis B surface antigen for chronic hepatitis B infection therapy.

  2022cites this paper
• Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models

  2021cites this paper
• Central Nervous System Delivery of Antibodies and Their Single-Domain Antibodies and Variable Fragment Derivatives with Focus on Intranasal Nose to Brain Administration

  2021cites this paper
• Direct‐Acting Antivirals for the Treatment of Chronic Hepatitis B Virus (HBV) Infection

  2021cites this paper
• Single-Domain Antibodies: Biology, Engineering and Emerging Applications

  2020cites this paper
• Selection and Characterization of Anti-idiotypic Shark Antibody Domains.

  2020cites this paper
• Diagnostic and therapeutic potential of shark variable new antigen receptor (VNAR) single domain antibody

  2020cites this paper
• Ancient species offers contemporary therapeutics: an update on shark VNAR single domain antibody sequences, phage libraries and potential clinical applications.

  2020cites this paper
• Applying Antibodies Inside Cells: Principles and Recent Advances in Neurobiology, Virology and Oncology

  2020cites this paper
• Characterization of Hepatitis B Precore/Core-Related Antigens

  2020cites this paper
• Genotype Phenotype Coupling: Methods and Protocols

  2020influential citation
• Shark IgNAR-derived binding domains as potential diagnostic and therapeutic agents.

  2019cites this paper
• Molecular Origin of the Stability Difference in Four Shark IgNAR Constant Domains.

  2019cites this paper
• Engineering partial resistance to cucumber mosaic virus in tobacco using intrabodies specific for the viral polymerase.

  2019cites this paper
• Analysis of the in vitro replication phenotype of African hepatitis B virus (HBV) genotypes and subgenotypes present in Australia identifies marked differences in DNA and protein expression.

  2019cites this paper
• Antibody Structure and Function: The Basis for Engineering Therapeutics

  2019cites this paper
• Hepatitis B virus core protein phosphorylation: Identification of the SRPK1 target sites and impact of their occupancy on RNA binding and capsid structure

  2018cites this paper
• The Development of Single Domain Antibodies for Diagnostic and Therapeutic Applications

  2018cites this paper
• In vitro studies identify a low replication phenotype for hepatitis B virus genotype H generally associated with occult HBV and less severe liver disease.

  2018cites this paper
• ALT flares during nucleotide analogue therapy are associated with HBsAg loss in genotype A HBeAg‐positive chronic hepatitis B

  2018cites this paper
• Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries.

  2018cites this paper
• Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments.

  2018cites this paper
• Next-generation flexible formats of VNAR domains expand the drug platform's utility and developability.

  2018cites this paper
• Single-domain antibody fragments derived from heavy-chain antibodies: a review

  2018cites this paper
• Camelid and shark single domain antibodies: structural features and therapeutic potential.

  2017cites this paper
• Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis

  2017cites this paper
• Phage Display Derived IgNAR V Region Binding Domains for Therapeutic Development.

  2017cites this paper
• Single Domain Antibodies as New Biomarker Detectors

  2017cites this paper
• Expression of wild-type or G1862T mutant HBe antigen of subgenotype A1 of hepatitis B virus and the unfolded protein response in Huh7 cells.

  2017influential citation
• The Basis for Antiviral Therapy: Drug Targets, Cross-Resistance, and Novel Small Molecule Inhibitors

  2016cites this paper
• Hepatitis B e Antigen Expression by Hepatitis B Virus Subgenotype A1 Relative to Subgenotypes A2 and D3 in Cultured Hepatocellular Carcinoma (Huh7) Cells

  2016cites this paper
• Recent Advances with ER Targeted Intrabodies.

  2016cites this paper
• Allosteric inhibition of Aurora-A kinase by a synthetic vNAR domain

  2016cites this paper
• In Vitro Studies Show that Sequence Variability Contributes to Marked Variation in Hepatitis B Virus Replication, Protein Expression, and Function Observed across Genotypes

  2016cites this paper
• Single-domain antibodies for biomedical applications

  2016cites this paper
• i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4*

  2016cites this paper
• VNARs: An Ancient and Unique Repertoire of Molecules That Deliver Small, Soluble, Stable and High Affinity Binders of Proteins

  2015cites this paper
• Specific in vivo knockdown of protein function by intrabodies

  2015cites this paper
• Elucidating the role of the HBV e antigen in manipulating the innate immune response

  2015cites this paper
• Structural and genetic diversity in antibody repertoires from diverse species.

  2015cites this paper
• Intracellular Acidosis Promotes Mitochondrial Apoptosis Pathway: Role of EMMPRIN Down-regulation via Specific Single-chain Fv Intrabody

  2015cites this paper
• Thermal stability and refolding capability of shark derived single domain antibodies.

  2014cites this paper
• What the shark immune system can and cannot provide for the expanding design landscape of immunotherapy

  2014cites this paper
• Phage display creates innovative applications to combat hepatitis B virus.

  2014cites this paper
• Shark variable new antigen receptor biologics – a novel technology platform for therapeutic drug development

  2014cites this paper
• Structural insights and biomedical potential of IgNAR scaffolds from sharks

  2014cites this paper
• Engineered Antibody Domains as Candidate Therapeutics

  2014cites this paper
• Monoclonal antibodies for prophylactic and therapeutic use against viral infections

  2013cites this paper
• Anti-tumor necrosis factor VNAR single domains reduce lethality and regulate underlying inflammatory response in a murine model of endotoxic shock

  2013cites this paper
• Monoclonal antibodies for prophylactic and therapeutic use against viral infections

  2013cites this paper
• eview onoclonal antibodies for prophylactic and therapeutic use against viral

  2013cites this paper
• Hepatitis B Precore Protein: Pathogenic Potential and Therapeutic Promise

  2012cites this paper
• Biotechnological applications of recombinant single-domain antibody fragments

  2011cites this paper
• Biotechnological applications of recombinant single-domain antibody fragments

  2011cites this paper
• marine drugs

  year unknowncites this paper