Dual site-controlled two-photon fluorescent probe for the imaging of lysosomal pH in living cells.

Abnormal lysosomal pH is closely associated with many diseases, and real-time monitoring of lysosomal pH is important for understanding the lysosome physiological nature. Here, we present a novel lysosome-targeting two-photon fluorescent probe (MP-lys) for monitoring pH changes in living cells. As a dual site-controlled probe, MP-lys employed morpholine and piperazine groups as the lysosome-targeting groups and pH response sites. MP-lys showed rapid, reversible and sensitive fluorescence response to pH. MP-lys possessed lysosome-targeting properties, and could be used for two-photon imaging of chloroquine-induced pH variation in living cells.

• Publication year

  2018

• Venue

  Luminescence (Chichester, England Print)

• Publication date

  2018-09-06

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1002/bio.3546PMID 30256510
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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