Assessing stability and assembly of the hepatitis B surface antigen into virus-like particles during down-stream processing.

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Vaccine

• Publication date

  2015-07-17

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.vaccine.2015.05.066PMID 26079614
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris and in vivo analysis of their immunogenic properties.

  2013cited by this paper
• Novel membrane extraction procedure for the purification of hepatitis B surface antigen from Pichia pastoris.

  2012cited by this paper
• Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

  2011cited by this paper
• Virus-like particles in vaccine development

  2010cited by this paper
• Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns

  2010cited by this paper
• Expression, Purification, and Characterization of Hepatitis B Virus Surface Antigens (HBsAg) in Yeast Pichia Pastoris

  2009cited by this paper
• Exploiting the intracellular compartmentalization characteristics of the S. cerevisiae host cell for enhancing primary purification of lipid‐envelope virus‐like particles

  2009cited by this paper
• Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

  2009cited by this paper
• Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

  2009cited by this paper
• Extrinsic Fluorescent Dyes as Tools for Protein Characterization

  2008cited by this paper
• A highly efficient integrated chromatographic procedure for the purification of recombinant hepatitis B surface antigen from Hansenula polymorpha.

  2007cited by this paper
• Expression of hepatitis B surface antigen major subtypes in Pichia pastoris and purification for in vitro diagnosis.

  2007cited by this paper
• Virus-like particles: Passport to immune recognition

  2006cited by this paper
• Maturation of Recombinant Hepatitis B Virus Surface Antigen Particles

  2006cited by this paper
• Expression in and purification of Hepatitis B surface antigen (S-protein) from methylotrophic yeast Pichia pastoris.

  2006cited by this paper
• Blue silver: A very sensitive colloidal Coomassie G‐250 staining for proteome analysis

  2004cited by this paper
• Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris.

  2001cited by this paper
• Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris.

  2000cited by this paper
• Comparison between hepatitis B surface antigen (HBsAg) particles derived from mammalian cells (CHO) and yeast cells (Hansenula polymorpha): composition, structure and immunogenicity.

  1997cited by this paper
• Saccharomyces cerevisiae can release hepatitis B virus surface antigen (HBsAg) particles into the medium by its secretory apparatus

  1993cited by this paper
• Adsorption–desorption of recombinant hepatitis B surface antigen (r‐HBsAg) from P. pastoris on a diatomaceous earth matrix: Optimization of parameters for purification

  1993cited by this paper
• Study of the “molten globule” intermediate state in protein folding by a hydrophobic fluorescent probe

  1991cited by this paper
• Folding of a predominantly beta-structure protein: rat intestinal fatty acid binding protein.

  1990cited by this paper
• Recombinant versus plasma-derived hepatitis B vaccines: issues of safety, immunogenicity and cost-effectiveness.

  1988cited by this paper
• High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris

  1987cited by this paper
• Multiple chemical forms of hepatitis B surface antigen produced in yeast.

  1985cited by this paper
• Measurement of protein using bicinchoninic acid.

  1985cited by this paper
• Human hepatitis B vaccine from recombinant yeast

  1984cited by this paper
• Intracellular transport and secretion of hepatitis B surface antigen in mammalian cells

  1984cited by this paper
• Structure of hepatitis B surface antigen. Characterization of the lipid components and their association with the viral proteins.

  1982cited by this paper
• Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

  1982cited by this paper

• Optimizing Primary Isolation of Recombinant HPV L1 Protein type 58 from Hansenula polymorpha

  2025cites this paper
• A novel method for recombinant mammalian‐expressed S‐HBsAg virus‐like particle production for assembly status analysis and improved anti‐HBs serology

  2024cites this paper
• Protein Expression Platforms and the Challenges of Viral Antigen Production

  2024cites this paper
• Selective extraction of recombinant membrane proteins from Hansenula polymorpha by pulsed electric field and lytic enzyme pretreatment

  2023cites this paper
• Immunogenicity of Wild Type and Mutant Hepatitis B Surface Antigen Virus-like Particles (VLPs) in Mice with Pre-Existing Immunity against the Wild Type Vector

  2023cites this paper
• HBV Vaccines: Advances and Development

  2023cites this paper
• Determining buffer conditions for downstream processing of VLP-based recombinant hepatitis B surface antigen using multimodal resins in bind-elute and flow-through purification modes

  2023cites this paper
• Quantitative assessment of LPS-HBsAg interaction by introducing a novel application of immunoaffinity chromatography

  2022cites this paper
• Hepatitis B Virus (HBV) Subviral Particles as Protective Vaccines and Vaccine Platforms

  2020cites this paper
• Solid-State NMR for Studying the Structure and Dynamics of Viral Assemblies

  2020cites this paper
• Characterization of hepatitis B virus surface antigen particles expressed in stably transformed mammalian cell lines containing the large, middle and small surface protein.

  2020cites this paper
• Assessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis B surface antigen from Pichia pastoris.

  2019cites this paper
• Integration of size-exclusion chromatography and ultracentrifugation for purification of recombinant hepatitis B surface antigen: An alternative method for immunoaffinity chromatography

  2019cites this paper
• Yeast-based vaccines: New perspective in vaccine development and application.

  2019cites this paper
• Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.

  2019cites this paper
• A novel application of ion exchange chromatography in recombinant hepatitis B vaccine downstream processing: Improving recombinant HBsAg homogeneity by removing associated aggregates.

  2019cites this paper
• Impact of hepatitis B virus genotype F on in vitro diagnosis: detection efficiency of HBsAg from Amerindian subgenotypes F1b and F4

  2019cites this paper
• Structural Studies of Self-Assembled Subviral Particles: Combining Cell-Free Expression with 110 kHz MAS NMR Spectroscopy.

  2018cites this paper
• Comparison of the size distributions and immunogenicity of human papillomavirus type 16 L1 virus-like particles produced in insect and yeast cells

  2018cites this paper
• Towards structural studies of self-assembled subviral particles: combining cell-free expression with 100 kHz MAS NMR.

  2018cites this paper
• Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography

  2018cites this paper
• Improving the recovery of clarification process of recombinant hepatitis B surface antigen in large-scale by optimizing adsorption-desorption parameters on Aerosil-380

  2018influential citation
• Yeast as an expression system for producing virus‐like particles: what factors do we need to consider?

  2017cites this paper
• Comparison of the patterns of antibody recall responses to HIV-1 gp120 and hepatitis B surface antigen in immunized mice.

  2016cites this paper
• Prospects of the use of bacteriophage-based virus-like particles in the creation of anthrax vaccines

  2016cites this paper