Insight into the α-Amylase Inhibitory Activity of Plant Lipid Transfer Proteins

We previously characterized the inhibitory activity of human salivary α-amylase (HSA) and Callosobruchus maculatus intestinal α-amylases by the plant lipid transfer protein from Vigna unguiculata ( Vu-LTP). Herein, we further study this inhibitory activity. First by an analysis of protein α-amylase inhibitors complexed with α-amylase, we find that positively charged amino acids of inhibitors interact with the active site of α-amylases and we know that Vu-LTP is rich in positively charged amino acid residues. For this reason, we model Vu-LTP, and based on its three-dimensional structure, we choose five peptides to be synthesized. Herein, we report that two peptides of Vu-LTP are responsible for HSA inhibition. A comparison of primary and tertiary structures of LTPs with and without inhibitory activity against α-amylase, superimposed with the sequence of Vu-LTP mapped for HSA inhibition, reinforces our suggestion that positively charged amino acids in loops are responsible for the inhibition. To prove our observation, one modified peptide is synthesized in which Arg39 is replaced by Gln. This modified peptide loses the HSA inhibitory property presented by the unmodified peptide. Therefore, we describe a new biological active for Vu-LTP, i.e. the α-amylase inhibitory activity that is not a fortuitous biological activity and probably has evolved to perform a biological function which is still unknown. A good candidate should be defense against insects. The results of this study also expand the possible biotechnological applications of LTPs.

• Publication year

  2018

• Venue

  Journal of Chemical Information and Modeling

• Publication date

  2018-11-02

• Fields of study

  Biology, Chemistry, Computer Science, Environmental Science, Medicine

• Identifiers
  DOI 10.1021/acs.jcim.8b00540PMID 30388003
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Recombinant production and α-amylase inhibitory activity of the lipid transfer protein from Vigna unguiculata (L. Walp.) seeds

  2017cited by this paper
• A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties

  2016cited by this paper
• Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling

  2016cited by this paper
• A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice.

  2015cited by this paper
• BraLTP1, a Lipid Transfer Protein Gene Involved in Epicuticular Wax Deposition, Cell Proliferation and Flower Development in Brassica napus

  2014cited by this paper
• Modeling, docking and dynamics simulations of a non-specific lipid transfer protein from Peganum harmala L

  2013cited by this paper
• Computational evaluation on the binding affinity of non-specific lipid-transfer protein-2 with fatty acids

  2013cited by this paper
• Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat

  2012cited by this paper
• Insight into the Molecular Evolution of Non-Specific Lipid Transfer Proteins via Comparative Analysis Between Rice and Sorghum

  2012cited by this paper
• Spectroscopic and Computational Study of Melittin, Cecropin A, and the Hybrid Peptide CM15

  2012cited by this paper
• Characterisation, immunolocalisation and antifungal activity of a lipid transfer protein from chili pepper (Capsicum annuum) seeds with novel α-amylase inhibitory properties.

  2011cited by this paper
• Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

  2011cited by this paper
• Purification, biochemical characterization and antifungal activity of a new lipid transfer protein (LTP) from Coffea canephora seeds with α-amylase inhibitor properties.

  2011cited by this paper
• Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor.

  2010cited by this paper
• The Role of Lipid Transfer Proteins in Allergic Diseases

  2010cited by this paper
• Identification of genes differentially expressed in cauliflower associated with resistance to Xanthomonas campestris pv. campestris

  2010cited by this paper
• Unexpected localization of a lipid transfer protein in germinating sunflower seeds.

  2009cited by this paper
• Cloning and expression analysis of 14 lipid transfer protein genes from Tamarix hispida responding to different abiotic stresses.

  2009cited by this paper
• The influence of barley malt protein modification on beer foam stability and their relationship to the barley dimeric alpha-amylase inhibitor-I (BDAI-I) as a possible foam-promoting protein.

  2008cited by this paper
• Role of plant lipid transfer proteins in plant cell physiology-a concise review.

  2007cited by this paper
• Structure‐based protein engineering for α‐amylase inhibitory activity of plant defensin

  2007cited by this paper
• Solution structure of the plant defensin VrD1 from mung bean and its possible role in insecticidal activity against bruchids

  2006cited by this paper
• Cloning and characterization of a cowpea seed lipid transfer protein cDNA: expression analysis during seed development and under fungal and cold stresses in seedlings' tissues.

  2006cited by this paper
• Impact of different wort boiling temperatures on the beer foam stabilizing properties of lipid transfer protein 1.

  2004cited by this paper
• Intracellular localization of a lipid transfer protein in Vigna unguiculata seeds

  2004cited by this paper
• Parmodel: a web server for automated comparative modeling of proteins.

  2004cited by this paper
• Evaluation of plant non-specific lipid-transfer proteins for potential application in drug delivery

  2004cited by this paper
• Proteinaceous α-amylase inhibitors

  2004cited by this paper
• Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity.

  2003cited by this paper
• Synthetic peptides derived from the beta2-beta3 loop of Raphanus sativus antifungal protein 2 that mimic the active site.

  2001cited by this paper
• Binding of two mono-acylated lipid monomers by the barley lipid transfer protein, LTP1, as viewed by fluorescence, isothermal titration calorimetry and molecular modelling.

  2001cited by this paper
• Solution structure of a lipid transfer protein extracted from rice seeds. Comparison with homologous proteins.

  2001cited by this paper
• Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography.

  2001cited by this paper
• Bean alpha-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions.

  2000cited by this paper
• Purification, characterization and antifungal properties of a lipid‐transfer protein from sunflower (Helianthus annuus) seeds

  2000cited by this paper
• The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins

  1999cited by this paper
• The crystal structure of a wheat nonspecific lipid transfer protein (ns-LTP1) complexed with two molecules of phospholipid at 2.1 A resolution.

  1999cited by this paper
• Rice non-specific lipid transfer protein: the 1.6 A crystal structure in the unliganded state reveals a small hydrophobic cavity.

  1998cited by this paper
• Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins.

  1998cited by this paper
• Characterisation of acyl binding by a plant lipid-transfer protein.

  1998cited by this paper
• Cysteine-to-alanine replacements in the Escherichia coli SoxR protein and the role of the [2Fe-2S] centers in transcriptional activation.

  1997cited by this paper
• Mutational Analysis of a Plant Defensin from Radish (Raphanus sativus L.) Reveals Two Adjacent Sites Important for Antifungal Activity*

  1997cited by this paper
• LIPID-TRANSFER PROTEINS IN PLANTS.

  1996cited by this paper
• The crystal structure of porcine pancreatic alpha-amylase in complex with the microbial inhibitor Tendamistat.

  1995cited by this paper
• A Potent Antimicrobial Protein from Onion Seeds Showing Sequence Homology to Plant Lipid Transfer Proteins

  1995cited by this paper
• Reference points for comparisons of two‐dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions

  1994cited by this paper
• Identification of a lipid transfer protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves.

  1994cited by this paper
• Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. A new folding for lipid carriers.

  1994cited by this paper
• Purification and antipathogenic activity of lipid transfer proteins (LTPs) from the leaves of Arabidopsis and spinach

  1993cited by this paper
• Comparative protein modelling by satisfaction of spatial restraints.

  1993cited by this paper
• Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens

  1993cited by this paper
• The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

  1993cited by this paper
• In Vitro Antifungal Activity of a Radish (Raphanus sativus L.) Seed Protein Homologous to Nonspecific Lipid Transfer Proteins.

  1992cited by this paper
• Nonspecific lipid transfer protein in castor bean cotyledon cells: subcellular localization and a possible role in lipid metabolism.

  1992cited by this paper
• Isolation of a cDNA Clone for Spinach Lipid Transfer Protein and Evidence that the Protein Is Synthesized by the Secretory Pathway.

  1991cited by this paper
• Cell-specific expression of the carrot EP2 lipid transfer protein gene.

  1991cited by this paper
• A synthetic 13-residue peptide corresponding to the hydrophobic region of bovine seminalplasmin has antibacterial activity and also causes lysis of red blood cells.

  1990cited by this paper
• Coidentity of putative amylase inhibitors from barley and finger millet with phospholipid transfer proteins inferred from amino acid sequence homology.

  1989cited by this paper
• Amino acid sequence of a probable amylase/protease inhibitor from rice seeds.

  1988cited by this paper
• Purification and characterization of a spinach-leaf protein capable of transferring phospholipids from liposomes to mitochondria or chloroplasts.

  1984cited by this paper
• The complete amino acid sequence of the α‐amylase inhibitor I‐2 from seeds of ragi (Indian finger millet, Eleusine coracana Gaertn.)

  1984cited by this paper
• [17] Amylases, α and β

  1955cited by this paper

• Integrated Biochemical, Proteomic, and Structural Characterization of Amylase Trypsin Inhibitor Proteins from Wheat (Triticum aestivum) Variety HD 3086.

  2025cites this paper
• The 2S albumin‐like protein from Amaranthus disrupts insect metabolism and triggers antibiosis

  2025cites this paper
• Lipid Transfer Proteins (LTPs) Partially Purified from Capsicum chinense Jacq. Seeds: Antifungal Properties and α-amylase Inhibitory Activity

  2025cites this paper
• A highly efficient method for screening dual-target inhibitory peptides against angiotensin I-converting enzyme and α-amylase from Chlorella using an enzyme-immobilized affinity membrane.

  2025cites this paper
• Roles of non-specific lipid transfer proteins in plant defense: structural and functional perspectives

  2025cites this paper
• Organelle Ca2+ /CAM1-SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration.

  2024cites this paper
• An Effective Plant Small Secretory Peptide Recognition Model Based on Feature Correction Strategy

  2023cites this paper
• Sequence Analysis and Biochemical Characteristics of Two Non-specific Lipid Transfer Proteins from Tartary Buckwheat Seeds.

  2023cites this paper
• Identification of a novel α-amylase inhibitory activity peptide from quinoa protein hydrolysate.

  2022cites this paper
• Structure, Function and Protein Engineering of Cereal-Type Inhibitors Acting on Amylolytic Enzymes

  2022cites this paper
• Antimicrobial peptides of the genus Capsicum: a mini review

  2022cites this paper
• Identification of enzyme inhibitors and antimicrobial activities from Capsicum annuum L. protein extracts against Colletotrichum scovillei

  2021cites this paper
• Proteinaceous α-amylase inhibitors: purification, detection methods, types and mechanisms

  2021cites this paper
• Identification of enzyme inhibitors and antimicrobial activities from Capsicum annuum L. protein extracts against Colletotrichum scovillei

  2021cites this paper
• Biotechnological Applications of Versatile Plant Lipid Transfer Proteins (LTPs).

  2021cites this paper
• Lipid Transfer Proteins (LTPs)—Structure, Diversity and Roles beyond Antimicrobial Activity

  2021cites this paper
• Bifunctional Inhibitors from Capsicum chinense Seeds with Antimicrobial Activity and Specific Mechanism of Action against Phytopathogenic Fungi.

  2020cites this paper
• The interaction of α-amylase with mycoprotein: Diffusion through the fungal cell wall, enzyme entrapment, and potential physiological implications

  2020cites this paper
• Inhibition mechanism of human salivary α-amylase by lipid transfer protein from Vigna unguiculata

  2019cites this paper