Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.

A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

• Publication year

  2019

• Venue

  Organic and biomolecular chemistry

• Publication date

  2019-02-13

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1039/c8ob02467gPMID 30468226PMCID PMC6503966
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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