Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Journal of Clinical Microbiology

• Publication date

  2005-10-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/JCM.43.10.5366-5368.2005PMID 16208020PMCID PMC1248492
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparison of Real-Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme-Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis

  2005cited by this paper
• Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (1→3)-β-d-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorders

  2004cited by this paper
• Development of a Serum-Based Taqman Real-Time PCR Assay for Diagnosis of Invasive Aspergillosis

  2004cited by this paper
• Prospective clinical evaluation of a LightCyclerTM‐mediated polymerase chain reaction assay, a nested‐PCR assay and a galactomannan enzyme‐linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients

  2004cited by this paper
• Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients

  2003cited by this paper
• Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis

  2003cited by this paper
• Comparison of Real-Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme-Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis

  2003cited by this paper
• Real-time automated polymerase chain reaction (PCR) to detect Candida albicans and Aspergillus fumigatus DNA in whole blood from high-risk patients.

  2003cited by this paper
• Detection of seven Candida species using the Light-Cycler system.

  2003cited by this paper
• Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

  2002cited by this paper
• Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis.

  2001cited by this paper
• Decision to delivery for assisted vaginal vertex delivery.

  2001cited by this paper
• Diagnosis of invasive aspergillosis in bone marrow transplant recipients by polymerase chain reaction

  2000cited by this paper
• Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System

  2000cited by this paper
• Specific Detection of Aspergillus Species in Blood and Bronchoalveolar Lavage Samples of Immunocompromised Patients by Two-Step PCR

  1999cited by this paper

• Invasive aspergillosis in adult patients in Australia and New Zealand: 2017–2020

  2023cites this paper
• Infections of the Cornea and Conjunctiva

  2021cites this paper
• Fungal Keratitis

  2020cites this paper
• Auditing fungal disease in leukemia patients in a tertiary care center: opportunities and challenges for an antifungal stewardship program

  2019influential citation
• Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.

  2019cites this paper
• Diagnostic Molecular Microbiology and its applications: Current and Future Perspectives

  2016cites this paper
• Publications for Tania Sorrell

  2016cites this paper
• Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.

  2015cites this paper
• Development of a Novel Inhalational Model of Invasive Pulmonary Aspergillosis in Rats and Comparative Evaluation of Three Biomarkers for Its Diagnosis

  2014cites this paper
• Clinical utility of Aspergillus galactomannan and PCR in bronchoalveolar lavage fluid for the diagnosis of invasive pulmonary aspergillosis in patients with haematological malignancies.

  2014cites this paper
• Respiratory fungal infections: molecular diagnostic tests.

  2014cites this paper
• Galactomannan and PCR versus culture and histology for directing use of antifungal treatment for invasive aspergillosis in high-risk haematology patients: a randomised controlled trial.

  2013cites this paper
• Laboratory Diagnosis of Invasive Aspergillosis: From Diagnosis to Prediction of Outcome

  2013cites this paper
• Clinical Significance of Quantitative Analysis of Plasma Epstein-Barr Virus DNA in Patients of Xinjiang Uygur Nationality with Hodgkin ’ s Lymphoma

  2013cites this paper
• Clinical significance of quantitative analysis of plasma epstein- barr virus DNA in patients of Xinjiang Uygur nationality with Hodgkin's Lymphoma.

  2012cites this paper
• A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection

  2012cites this paper
• Specific detection of Aspergillus species in blood from patients with invasive aspergillosis.

  2012cites this paper
• A Novel Extraction Method Combining Plasma with a Whole-Blood Fraction Shows Excellent Sensitivity and Reproducibility for Patients at High Risk for Invasive Aspergillosis

  2012cites this paper
• Quantitative nested real-time PCR detection of Verticillium longisporum and V. dahliae in the soil of cabbage fields

  2011cites this paper
• Emerging moulds: epidemiological trends and antifungal resistance

  2011cites this paper
• Design issues in a randomized controlled trial of a pre-emptive versus empiric antifungal strategy for invasive aspergillosis in patients with high-risk hematologic malignancies

  2011cites this paper
• Development of a Real-Time qPCR Method for Detection and Enumeration of Mycobacterium spp. in Surface Water

  2010cites this paper
• DNA-Based Detection of Human Pathogenic Fungi: Dermatophytes, Opportunists, and Causative Agents of Deep Mycoses

  2010cites this paper
• Quantification of Nitrogen Reductase and Nitrite Reductase Genes in Soil of Thinned and Clear-Cut Douglas-Fir Stands by Using Real-Time PCR

  2010cites this paper
• Nested PCR bias: a case study of Pseudomonas spp. in soil microcosms.

  2010cites this paper
• Evaluation of the clinical cutoff level of serum (1 → 3)-β-d-glucan in patients with connective tissue diseases complicated by deep fungal infections

  2010cites this paper
• Evaluation of the clinical cutoff level of serum (1 → 3)-β-d-glucan in patients with connective tissue diseases complicated by deep fungal infections

  2010cites this paper
• Current status and future perspectives on molecular and serological methods in diagnostic mycology.

  2009cites this paper
• Molecular ecological analysis of methanogenic communities in terrestrial and submarine permafrost deposits of Siberian Laptev Sea area

  2009cites this paper
• Preemptive antifungal therapy: Do diagnostics help?

  2009cites this paper
• Molecular diagnostic platforms for detecting Aspergillus.

  2009cites this paper
• Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance

  2009cites this paper
• Proviral DNA Unspliced and Multiply Spliced RNA and Human Immunodeficiency Virus Type 1 Transcription-PCR for Quantitation of Seminested Real-Time Reverse Highly Sensitive Methods Based on

  2008cites this paper
• Challenges and progress in setting a standard for PCR for invasive aspergillosis

  2008cites this paper
• Highly sensitive methods for quantitation of HIV-1 unspliced and multiply spliced RNA and proviral DNA based on semi-nested real-time RT-PCR

  2008cites this paper
• Highly Sensitive Methods Based on Seminested Real-Time Reverse Transcription-PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA

  2008cites this paper
• Successful control of an outbreak of invasive aspergillosis in a regional haematology unit during hospital construction works.

  2008cites this paper
• Diagnostic and therapeutic approach to persistent or recurrent fevers of unknown origin in adult stem cell transplantation and haematological malignancy

  2008cites this paper
• Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens

  2007cites this paper
• Real‐Time PCR Assay to Detect DNA in Sera for the Diagnosis of Deep‐Seated Trichosporonosis

  2007cites this paper
• Molecular and serological diagnosis of invasive aspergillosis: new answers to old questions?

  2007cites this paper
• Reverse Line Blot Hybridization Assay for Identification of Medically Important Fungi from Culture and Clinical Specimens (cid:1)

  2007cites this paper
• Aspergillus PCR - Platforms, strengths and weaknesses.

  2006cites this paper
• Antifungal strategies for managing invasive aspergillosis: The prospects for a pre-emptive treatment strategy.

  2006cites this paper
• Molecular diagnosis of invasive aspergillosis: the long and winding road.

  2006cites this paper
• Role of prospective screening of blood for invasive aspergillosis by polymerase chain reaction in febrile neutropenic recipients of haematopoietic stem cell transplants and patients with acute leukaemia

  2005cites this paper
• A Nested PCR Assay to Detect DNA in Sera for the Diagnosis of Deep‐Seated Trichosporonosis

  2001cites this paper