Many plant species respond to herbivore attack by an increased formation of volatile organic compounds. In this preliminary study we analysed the volatile metabolome of grapevine roots [Teleki 5C (Vitis berlandieri Planch. × Vitis riparia Michx.)] with the aim to gain insight into the interaction between phylloxera (Daktulosphaira vitifoliae Fitch; Hemiptera: Phylloxeridae) and grapevine roots. In the first part of the study, headspace solid phase microextraction (HS-SPME) coupled to gas chromatography – mass spectrometry (GC–MS) was used to detect and identify volatile metabolites in uninfested and phylloxera-infested root tips of the grapevine rootstock Teleki 5C. Based on the comparison of deconvoluted mass spectra with spectra databases as well as experimentally derived retention indices with literature values, 38 metabolites were identified, which belong to the major classes of plant volatiles including C6-compounds, terpenes (including modified terpenes), aromatic compounds, alcohols and n-alkanes. Based on these identified metabolites, changes in root volatiles were investigated and resulted in metabolite profiles caused by phylloxera infestation. Our preliminary data indicate that defence related pathways such as the mevalonate and/or alternative isopentenyl pyrophosphate-, the lipoxygenase- (LOX) as well as the phenylpropanoid pathway are affected in root galls as a response to phylloxera attack.
The volatile metabolome of grapevine roots: First insights into the metabolic response upon phylloxera attack
N. C. Lawo,G. Weingart,R. Schuhmacher,A. Forneck
Published 2011 in Plant physiology and biochemistry : PPB
ABSTRACT
PUBLICATION RECORD
- Publication year
2011
- Venue
Plant physiology and biochemistry : PPB
- Publication date
2011-09-01
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- alternative isopentenyl pyrophosphate pathway
A non-mevalonate pathway for isoprenoid precursor biosynthesis in plants.
Aliases: alternative IPP pathway, non-mevalonate pathway
- gc-ms
Gas chromatography-mass spectrometry, an analytical platform used to separate and identify volatile metabolites.
Aliases: gas chromatography-mass spectrometry, GC–MS
- grapevine roots
Root tissue of grapevine sampled for volatile metabolome analysis.
Aliases: root tips
- hs-spme
Headspace solid phase microextraction, an extraction method used to collect volatile compounds from root samples.
Aliases: headspace solid phase microextraction
- lipoxygenase pathway
A plant oxylipin-producing pathway associated with lipid-derived volatile metabolism.
Aliases: LOX pathway, LOX
- mevalonate pathway
A plant isoprenoid biosynthetic pathway that produces precursors for terpenoid metabolism.
Aliases: MVA pathway
- phenylpropanoid pathway
A plant secondary-metabolism pathway that generates aromatic phenylpropanoid compounds.
- phylloxera infestation
Colonization of grapevine roots by phylloxera used here as the biotic stress condition.
Aliases: phylloxera attack, phylloxera-infested
- root galls
Swollen or gall-like root structures formed on grapevine roots after phylloxera feeding.
Aliases: galls
- teleki 5c
A grapevine rootstock, derived from Vitis berlandieri and Vitis riparia, used as the plant material in the analysis.
Aliases: Teleki 5C (Vitis berlandieri Planch. × Vitis riparia Michx.)
- volatile metabolites
Low-molecular-weight volatile organic compounds detected in grapevine root material.
Aliases: volatile organic compounds, root volatiles
REFERENCES
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