Biochar Alteration of the Sorption of Substrates and Products in Soil Enzyme Assays

Pine wood and barley straw biochar amendments to Kettering and Cameroon sandy silt loam soils (15, 30, or 150 mg biochar g−1 soil) caused significant reductions (up to 80%, ) in concentrations of substrate and extractable product in soil dehydrogenase and phosphomonoesterase enzyme assays. Likely this was caused by increased solid-phase sorption of the chemicals in the presence of the biochars under assay conditions. The relationship between assay chemical sorption and biochar concentration depended on the chemical, soil type, biochar type, and their interactions; hence, no uniform correction factor could be derived. This biochar impact on assay constituents will limit the identification of genuine biochar effects on soil enzymes. It is recommended that the assumption of saturating substrate concentrations be checked and that product standards be matrix-matched when conducting enzyme assays with biochar-amended soil.

• Publication year

  2013

• Venue

  Applied and Environmental Soil Science

• Publication date

  2013-09-30

• Fields of study

  Chemistry, Environmental Science

• Identifiers
  DOI 10.1155/2013/968682
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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