High-throughput discrimination of bacteria isolated from Astacus astacus and A. leptodactylus

Bacterial diseases and pathogens of crayfish are common, widespread, and occasionally causing serious mortalities. In order to take rapid measures for correct treatment of crayfish diseases, the turnover time and accuracy in bacterial identification is an issue. Bacteria isolated from tissues of apparently healthy Astacus astacus and A. leptodactylus were identified by the commercial phenotypic tests (API 20E) and by the matrix assisted laser induced desorption ionization connected to the time of flight mass spectrometry (MALDI-TOF MS). For Gram-negative rods, API 20E resulted in fewer species identifications than MALDI-TOF MS (5.2% versus 52.61%). The most frequently identified genus from A. astacus and A. leptodactylus was Pseudomonas spp.: API 20E (47.82%) and MALDI-TOF MS (52.17%). Both systems identified 60.86% of total isolates identically to the genus. Hafnia alvei was the only isolate for which API 20E and MALDI-TOF MS had a concordant reading to the species. MALDI-TOF MS proved to be a powerful, low-cost, rapid tool in bacterial genus identification. This is the first report of a direct comparison between the two systems for the identification of bacteria in crayfish, and also the first report on using MALDI-TOF MS for discrimination of freshwater crayfish bacterial isolates.

• Publication year

  2014

• Venue

  Knowledge and Management of Aquatic Ecosystems

• Publication date

  2014-02-10

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.1051/KMAE/2014005
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Optimization of Routine Identification of Clinically Relevant Gram-Negative Bacteria by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and the Bruker Biotyper

  2013cited by this paper
• Identification and classification of seafood‐borne pathogenic and spoilage bacteria: 16S rRNA sequencing versus MALDI‐TOF MS fingerprinting

  2013cited by this paper
• Current status of matrix-assisted laser desorption ionisation-time of flight mass spectrometry in the clinical microbiology laboratory

  2013cited by this paper
• MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension.

  2013cited by this paper
• Bacterial identification: from the agar plate to the mass spectrometer

  2013influential reference
• Comparison of two matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria.

  2013cited by this paper
• The crayfish plague pathogen Aphanomyces astaci Genetic diversity and adaptation to the host species

  2013cited by this paper
• A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level.

  2013influential reference
• Aeromonas hydrophila subsp. dhakensis Isolated from Feces, Water and Fish in Mediterranean Spain

  2012cited by this paper
• Assessing Corpus Callosum Changes in Alzheimer's Disease: Comparison between Tract-Based Spatial Statistics and Atlas-Based Tractography

  2012cited by this paper
• A Comparative Study of the Bacterial Flora of the Hemolymph of Callinectes sapidus

  2012cited by this paper
• A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains

  2012cited by this paper
• A polyphasic approach to study the intraspecific diversity of Yersinia ruckeri strains isolated from recent outbreaks in salmonid culture.

  2012cited by this paper
• The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories.

  2012cited by this paper
• Edwardsiella ictaluri as the causative agent of mortality in cultured Nile tilapia.

  2012cited by this paper
• Comparison of bacterial identification by MALDI-TOF mass spectrometry and conventional diagnostic microbiology methods: agreement, speed and cost implications

  2012cited by this paper
• Disease risks associated with the importation and release of non-native crayfish species into mainland Britain

  2012influential reference
• Update on the distribution of freshwater crayfish in Croatia

  2011cited by this paper
• The study of bacteria on artificially incubated noble crayfish eggs

  2011influential reference
• Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram-Negative Bacilli

  2011cited by this paper
• Determination of Microbial Diversity of Aeromonas Strains on the Basis of Multilocus Sequence Typing, Phenotype, and Presence of Putative Virulence Genes

  2011cited by this paper
• Accuracy of the API Campy system, the Vitek 2 Neisseria-Haemophilus card and matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of Campylobacter and related organisms.

  2011cited by this paper
• Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

  2011influential reference
• Diseases of crayfish: a review.

  2011influential reference
• The ecology and emergence of diseases in fresh waters

  2011cited by this paper
• Identification of clinical coagulase-negative staphylococci, isolated in microbiology laboratories, by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and two automated systems.

  2010cited by this paper
• High-Throughput Identification of Bacteria and Yeast by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry in Conventional Medical Microbiology Laboratories

  2010cited by this paper
• Comparison of MALDI TOF with conventional identification of clinically relevant bacteria.

  2010influential reference
• Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Bacterial Strains Routinely Isolated in a Clinical Microbiology Laboratory

  2010influential reference
• Characterization of microorganisms by MALDI mass spectrometry.

  2009cited by this paper
• Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

  2009cited by this paper
• A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus.

  2009influential reference
• Evaluation of Genotypic and Phenotypic Methods To Distinguish Clinical from Environmental Vibrio vulnificus Strains

  2009cited by this paper
• High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria

  2009cited by this paper
• Comprar Bacterial Fish Pathogens · Disease of Farmed and Wild Fish | Austin, B. | 9781402060687 | Springer

  2007cited by this paper
• Evidence for the classification of a crayfish pathogen as a member of the genus Coxiella

  2007cited by this paper
• PRELIMINARY INVESTIGATIONS OF DISEASE-CAUSING ORGANISMS IN THE WHITE-CLAWED CRAYFISH AUSTROPOTAMOBIUS PALLIPES COMPLEX FROM STREAMS OF NORTHERN ITALY

  2006cited by this paper
• Sanitary-pathological examination of Red Swamp Crayfish (Procambarus clarkii, Girard 1852) in the Reno Valley.

  2006cited by this paper
• The distribution pattern of freshwater crayfish in Croatia

  2004cited by this paper
• Recovery of a new biogroup of Yersinia ruckeri from diseased rainbow trout (Oncorhynchus mykiss, Walbaum).

  2003cited by this paper
• Histopathological survey of diseases and pathogens present in redclaw crayfish, Cherax quadricarinatus (Von Martens), cultured in Ecuador

  2002cited by this paper
• Synopsis of freshwater crayfish diseases and commensal organisms

  2002influential reference
• Histopathological surveys of the redclaw freshwater crayfish, Cherax quadricarinatus, in Australia

  1999cited by this paper
• Comparison of Fatty Acid Methyl Ester Analysis with the Use of API 20E and NFT Strips for Identification of Aquatic Bacteria

  1996cited by this paper
• Gram negative bacteria in the hemolymph of noble crayfish Astacus astacus, in an intensive crayfish culture system.

  1996cited by this paper
• Pathogens, parasites and commensals.

  1988cited by this paper
• Bacterial flora of hemolymph from red swamp crawfish, Procambarus clarkii (Girard), from commercial ponds

  1986influential reference
• Bacterial infection in the blue crab, Callinectes sapidus: course of infection and histopathology

  1976cited by this paper

• Microbial Evaluation of Ozone Water Combined with Ultrasound Cleaning on Crayfish (Procambarus clarkii)

  2022cites this paper
• Cuticle-associated bacteria can inhibit crayfish pathogen Aphanomyces astaci: Opening the perspective of biocontrol in astaciculture

  2020cites this paper
• The Effect of Lipopolysaccharides on Primary Sensory Neurons in Crustacean Models

  2019cites this paper
• Epifauna of native and alien freshwater crayfish species (Crustacea:Decapoda): a host-specific community?

  2018cites this paper
• Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS.

  2017cites this paper
• Olive mill wastewater-enriched diet positively affects growth, oxidative and immune status and intestinal microbiota in the crayfish, Astacus leptodactylus

  2017cites this paper
• Parasites, Commensals, Pathogens and Diseases of Crayfi sh

  2016cites this paper
• Detection and diversity of aeromonads from treated wastewater and fish inhabiting effluent and downstream waters.

  2015cites this paper