A fluorescent aptamer/carbon dots based assay for Cytochrome c protein detection as a biomarker of cell apoptosis

Cytochrome c (Cyt c), a heme protein, can be a potential biomarker for cell-apoptosis or even cancer diagnosis. In this work, a simple, rapid, sensitive and selective label-free assay for Cytochrome c (Cyt c) detection is introduced based on an interaction between nucleic acid aptamer biomolecules and surfaces of Carbon Dots (CDs). CDs are used as a fluorescent probes and Cyt c-aptamers as a sensing materials. Interactions of aptamers with CDs quench the fluorescent intensity of CDs. By addition of Cyt c biomolecule as an analyte to the solution and binding to the aptamers, CDs fluorescence turns on. Stronger binding affinity of the aptamers toward Cyt c than CDs, causes they leave the CDs surfaces and the fluorescence is recovered. The amount of recoveries corresponds linearly to the concentration of Cyt c and be used as the basis of detection. The method exhibited high sensitivity to Cyt c with a detection limit of 25.90 nM and a linear range from 40 nM to 240 nM.

• Publication year

  2018

• Venue

  Methods and Applications in Fluorescence

• Publication date

  2018-12-17

• Fields of study

  Medicine, Physics, Chemistry

• Identifiers
  DOI 10.1088/2050-6120/aaf0caPMID 30524015
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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