The effects of antioxidants and nitric oxide modulators on hepatic ischemic-reperfusion injury in rats.

Ischemic-reperfusion injury (IRI) is thought to be caused by oxygen radicals. Nitric oxide (NO) also has been thought to play a key role in IRI. This experiment was designed to evaluate the effects of antioxidants and NO supplement on hepatic IRI. Male Sprague-Dawley rats were divided into five groups: a sham operation group, a group with IRI, and three groups with vitamin C combined with vitamin E (VC&VE), L-arginine and N(G)-nitro-L-arginine (NNLA) injected after IRI. IRI was induced by clamping of the porta hepatis for 30 minutes and then by declamping. To prevent mesenteric blood congestion, a porto-systemic shunt had been made four weeks before the portal clamping. Biochemical assays of TNF-alpha level and NO2- level in the blood, malondialdehyde level, catalase activity and NO synthase activity in the liver tissue were performed. The results were as follows: IRI increased the malondialdehyde level and exhausted the catalase activity remarkably. VC&VE lowered the malondialdehyde levels and protected against catalase exhaustion, but had no significant effect on the NO production. L-arginine had a definite antioxidant effect, which was much weaker than that of VC&VE. In conclusion, antioxidants and a supplement of NO protected the liver tissue against IRI.

• Publication year

  2002

• Venue

  Journal of Korean medical science

• Publication date

  2002-08-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.3346/JKMS.2002.17.4.502PMID 12172046PMCID 3054897
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Anti-EDRF effect of tumor necrosis factor in isolated, perfused cat carotid arteries.

  1989influential reference
• Antioxidant defenses and lipid peroxidation in human blood plasma.

  1988cited by this paper
• Protection against oxygen toxicity by intravenous injection of liposome-entrapped catalase and superoxide dismutase.

  1984cited by this paper
• Overview of methods used for detecting lipid peroxidation.

  1984cited by this paper
• Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems.

  1966cited by this paper
• A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.

  1952cited by this paper

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