Prairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST–SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.
Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop
K. Gedye,J. Gonzalez-Hernandez,Y. Ban,Xijin Ge,J. Thimmapuram,Fengjie Sun,C. Wright,Shahjahan Ali,A. Boe,V. Owens
Published 2010 in The Plant Genome
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- Publication year
2010
- Venue
The Plant Genome
- Publication date
2010-09-01
- Fields of study
Agricultural and Food Sciences, Biology, Environmental Science
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Semantic Scholar
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