A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

J. Sawicki,M. Szczecińska

Published 2011 in Acta Societatis Botanicorum Poloniae

ABSTRACT

RAPDs, ISJs, ISSRs, ITS and kat Gs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia . Twenty populations were genotyped using ten ISJ primers, 12 pairs of kat G primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and kat G markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.

PUBLICATION RECORD

  • Publication year

    2011

  • Venue

    Acta Societatis Botanicorum Poloniae

  • Publication date

    2011-07-29

  • Fields of study

    Biology, Environmental Science

  • Identifiers
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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