Effect of 2-bromoethanesulfonic acid on in vitro fermentation characteristics and methanogen population.

: An in vitro incubation study was conducted to investigate effects of 2-bromoethanesulfonic acid (BES) on ruminal fermentation characteristics and methanogen population. BES at the final concentration of 0, 1 and 5 mM with two different substrates having a different ratio of timothy and concentrate (100% timothy vs. 40% timothy-60% concentrate) was incubated for 0, 24, 48 and 72 h in a 39°C incubator. Total DNA extracted from culture fluid was used as a template for real-time PCR to measure the population of methanogens. Four different primer sets were used for amplification of total bacteria, total methanogens, the order Methanobacteriales and the order Methanomicrobiales. BES reduced (p<0.01) total gas and methane production in a dose-dependent manner. BES at 5 mM inhibited methane production by more than 95% compared to the control. An interaction between substrate and level of BES in total gas and methane was detected (p<0.01). The decrease of methane production with increasing BES level was more pronounced on mixed substrate than on timothy alone. However, hydrogen production was increased by BES treatment (p<0.01). Total VFA concentration was not affected, but molar percentage of propionate and butyrate was increased and acetate to propionate ratio was reduced by BES treatment (p<0.01). BES did not affect the population density of total bacteria but reduced (p<0.01) the population of total methanogens, the order Methanobacteriales and the order Methanomicrobiales in a dose-dependent manner. The type of substrate did not influence the trend, although the magnitude of response was different between all-roughage and 40% roughage substrate.

• Publication year

  2009

• Venue

  Asian-australasian Journal of Animal Sciences

• Publication date

  2009-01-06

• Fields of study

  Biology, Environmental Science

• Identifiers
  DOI 10.5713/AJAS.2009.80581
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Methane production from dairy cows

  2011cited by this paper
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  2008influential reference
• Control of Rumen Microbial Fermentation for Mitigating Methane Emissions from the Rumen

  2008cited by this paper
• Diets with Different Forage/Concentrate Ratios for the Mediterranean Italian Buffalo : In vivo and In vitro Digestibility

  2008cited by this paper
• Molecular Diversity of Methanogens in Feedlot Cattle from Ontario and Prince Edward Island, Canada

  2007influential reference
• Effects of Tween 80 Pretreatment on Dry Matter Disappearance of Rice Straw and Cellulolytic Bacterial Adhesion

  2007cited by this paper
• Analysis of Methanogen Diversity in the Rumen Using Temporal Temperature Gradient Gel Electrophoresis: Identification of Uncultured Methanogens

  2007influential reference
• Measurement of Methane Production from Ruminants

  2007cited by this paper
• Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane.

  2007cited by this paper
• Attenuation of ruminal methanogenesis

  2007cited by this paper
• Feed additives and other interventions for decreasing methane emissions

  2007cited by this paper
• Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen.

  2006influential reference
• 16S rDNA directed PCR primers and detection of methanogens in the bovine rumen

  2006influential reference
• Application of Recent DNA/RNA-based Techniques in Rumen Ecology

  2006cited by this paper
• Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction.

  2005cited by this paper
• Screening for effects of plant extracts and active compounds of plants on dairy cattle rumen microbial fermentation in a continuous culture system

  2005cited by this paper
• Effects of halogenated compounds, organic acids and unsaturated fatty acids on in vitro methane production and fermentation characteristics

  2004cited by this paper
• Effects of several inhibitors on pure cultures of ruminal methanogens *

  2004cited by this paper
• Methane Production Potential of Feed Ingredients as Measured by In Vitro Gas Test

  2003cited by this paper
• Phylogenetic analysis of archaeal 16S rRNA libraries from the rumen suggests the existence of a novel group of archaea not associated with known methanogens.

  2001influential reference
• Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets

  2001cited by this paper
• Effects of exogenous fibrolytic enzymes, α-bromoethanesulfonate and monensin on fermentation in a rumen simulation (RUSITEC) system

  1999cited by this paper
• Manipulation of ruminal fermentation with organic acids: a review.

  1998cited by this paper
• Effect of 2-bromoethanesulfonic acid and Peptostreptococcus productus ATCC 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis

  1997cited by this paper
• Microbial community structure in gastrointestinal tracts of domestic animals: comparative analyses using rRNA‐targeted oligonucleotide probes

  1997cited by this paper
• The effect of pH on ruminal methanogenesis

  1996cited by this paper
• Attempts to induce reductive acetogenesis into a sheep rumen.

  1996cited by this paper
• Dietary, environmental and microbiological aspects of methane production in ruminants

  1996cited by this paper
• A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds.

  1994cited by this paper
• Methane: global warming and production by animals

  1993cited by this paper
• Rumen ciliates: their metabolism and relationships with bacteria and their hosts

  1990cited by this paper
• Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid

  1988cited by this paper
• Changes in oxidation reduction potentials and volatile fatty acid production by rumen bacteria when methane synthesis is inhibited.

  1987cited by this paper
• The specificity of growth inhibition of methanogenic bacteria by bromoethanesulfonate

  1987cited by this paper
• Methane production by domestic animals, wild ruminants, other herbivorous fauna, and humans

  1986cited by this paper
• Effects of monensin, pyromellitic diimide and 2-bromoethanesulfonic acid on rumen fermentation in vitro.

  1985cited by this paper
• Association of methanogenic bacteria with rumen protozoa.

  1983cited by this paper
• Transport of coenzyme M (2-mercaptoethanesulfonic acid) in Methanobacterium ruminantium

  1979cited by this paper
• SAS user's guide

  1979cited by this paper
• MANIPULATION OF RUMEN FERMENTATION

  1977cited by this paper
• Effect of methane inhibitors on rumen metabolism and feedlot performance of sheep.

  1971cited by this paper
• Formate as an Intermediate in the Bovine Rumen Fermentation

  1970cited by this paper
• STATISTICAL METHODS

  1967cited by this paper
• The metabolism of oleic, linoleic and linolenic acids by sheep with reference to their effects on methane production

  1966cited by this paper
• Volatile Fatty Acid Analyses of Blood and Rumen Fluid by Gas Chromatography

  1961cited by this paper
• A Theoretical Rumen Fermentation Balance

  1960cited by this paper

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