Dmitriev A., M. Bhide, I. Mikula: cpn60 Gene Based Multiplex-PCR Assay for Simultaneous Identification of Streptococcal Species. Acta Vet. Brno 2006, 75: 235-240. The cpn60 genes of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis were sequenced and a certain polymorphism of cpn60 genes was revealed. Presumable species-specific pairs of primers were designed and their specificity was confirmed by PCR. Based on these data, the cpn60 gene-based multiplex-PCR assay was developed. It was found to be effective for simultaneous identification of S. agalactiae, S. dysgalactiae and S. uberis strains.
cpn60 Gene-Based Multiplex-PCR Assay for Simultaneous Identification of Streptococcal Species
A. Dmitriev,M. Bhide,I. Mikula
Published 2006 in Acta Veterinaria Brno
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2006
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Acta Veterinaria Brno
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Biology, Medicine
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