Studying arsenic trioxide-induced apoptosis of Colo-16 cells with two-photon and confocal laser scanning microscopy

With two-photon and confocal laser scanning microscopy in combination with fluorescent probes Hoechst 33342, 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and Fluo 3-AM, we simultaneously observed arsenic trioxide (As 2 O 3 )-induced changes in nuclear morphology, reactive oxygen species (ROS) and intracellular calcium concentration (Ca 2+ )i within human skin squamous carcinoma cells (Colo-16 cells). Our results indicated that As 2 O 3 induced (Ca 2+ )i elevation and ROS production within Colo-16 cells, and both (Ca 2+ )i elevation and ROS production were involved in the apoptosis of Colo-16 cells. These results suggested that two-photon and confocal laser scanning microscopy might provide a real-time, sensitive and noninvasive method for simultaneously multi-parameter observation of As 2 O 3 - induced apoptosis at the single cell level. Key words: Two-photon laser scanning microscopy, confocal laser scanning microscopy, human skin squamous carcinoma cells (Colo-16 cells), arsenic trioxide, apoptosis

• Publication year

  2011

• Venue

  African Journal of Biotechnology

• Publication date

  2011-10-05

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.5897/AJB10.2195
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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