The ribosomal repeat of Aspergillus niger and its effects on transformation frequency

The ribosomal RNA genes have been used in studies of a variety of phenomena. These include studies of molecular phylogeny, transcription, recombination and transformation. In Saccharomyces cerevisiae , where integrative transformation is achieved by homologous recombination, the presence of the ribosomal repeat unit in the transformation vector greatly increases the frequency of transformation (Szostak and Wu 1979. Plasmid 2:536-554; Smolik-Utlaut and Petes 1983. Mol. Cell. Biol. 3:1204-1211). In the filamentous fungus Aspergillus nidulans , where integrative transformation can be achieved either via homologous or heterologous recombination, the presence of ribosomal repeat sequences in the transformation vector has no effect on the frequency of transformation (Tilburn et al. 1983. Gene 26:205-221). [Ed.: This is also the case in Neurospora crassa : Russell et al. 1989. BBA 1008:243-246]. Here we report the molecular cloning of the ribosomal repeat unit from Aspergillus niger . We have found that the presence of cloned ribosomal DNA sequences from A. niger increases the frequency of transformation in A. niger .

• Publication year

  1990

• Venue

  Fungal Genetics Reports

• Publication date

  Unknown publication date

• Fields of study

  Biology

• Identifiers
  DOI 10.4148/1941-4765.1482
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• No references are available for this paper.

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