Surface apposition and multiple cell contacts promote myoblast fusion in Drosophila flight muscles

Transmission EM methods reveal that cell–cell fusion of individual myoblasts with growing Drosophila flight muscles is a stepwise process in which the cell adhesion and branched actin machineries mediate tight apposition and formation of multiple contacts and pores between the surfaces of the fusing cells.

• Publication year

  2015

• Venue

  Journal of Cell Biology

• Publication date

  2015-10-12

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201503005PMID 26459604PMCID 4602036
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Actin-delimited adhesion-independent clustering of E-cadherin forms the nanoscale building blocks of adherens junctions.

  2015cited by this paper
• Identification of singles bar as a direct transcriptional target of Drosophila Myocyte enhancer factor-2 and a regulator of adult myoblast fusion.

  2015cited by this paper
• Mechanical tension drives cell membrane fusion.

  2015cited by this paper
• Identification of a new stem cell population that generates Drosophila flight muscles

  2014cited by this paper
• Tethering Membrane Fusion: Common and Different Players in Myoblasts and at the Synapse

  2014cited by this paper
• Extracellular architecture of the SYG-1/SYG-2 adhesion complex instructs synaptogenesis.

  2014cited by this paper
• Extracellular annexins and dynamin are important for sequential steps in myoblast fusion

  2013cited by this paper
• Myomaker: A membrane activator of myoblast fusion and muscle formation

  2013cited by this paper
• Interplay between the dividing cell and its neighbors regulates adherens junction formation during cytokinesis in epithelial tissue.

  2013cited by this paper
• Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7.

  2013cited by this paper
• Genetic basis of cell-cell fusion mechanisms.

  2013cited by this paper
• Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography.

  2013cited by this paper
• The actin regulator N-WASp is required for muscle-cell fusion in mice

  2012cited by this paper
• Bidirectional Notch activation represses fusion competence in swarming adult Drosophila myoblasts

  2012cited by this paper
• New insights into the regulation and cellular functions of the ARP2/3 complex

  2012cited by this paper
• Fiji: an open-source platform for biological-image analysis

  2012cited by this paper
• Myoblast fusion: lessons from flies and mice

  2012cited by this paper
• Development and application of STEM for the biological sciences.

  2012cited by this paper
• The actin nucleator WASp is required for myoblast fusion during adult Drosophila myogenesis

  2011influential reference
• Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila

  2011cited by this paper
• Molecular mechanisms of myoblast fusion across species.

  2011cited by this paper
• 3D nuclear architecture reveals coupled cell cycle dynamics of chromatin and nuclear pores in the malaria parasite Plasmodium falciparum

  2011cited by this paper
• Invasive podosomes and myoblast fusion.

  2011cited by this paper
• Electron microscopy of the mouse central nervous system.

  2010cited by this paper
• Electron Tomography Reveals the Steps in Filovirus Budding

  2010cited by this paper
• Myoblast fusion: when it takes more to make one.

  2010cited by this paper
• Systematic genetic analysis of muscle morphogenesis and function in Drosophila

  2010cited by this paper
• An invasive podosome-like structure promotes fusion pore formation during myoblast fusion

  2010cited by this paper
• Myoblast fusion in Drosophila.

  2010cited by this paper
• Intracellular membrane traffic at high resolution.

  2010cited by this paper
• WASP and SCAR/WAVE proteins: the drivers of actin assembly

  2009cited by this paper
• Three-dimensional electron microscopy reveals new details of membrane systems for Ca2+ signaling in the heart

  2009cited by this paper
• Nap1-mediated actin remodeling is essential for mammalian myoblast fusion

  2009cited by this paper
• The SCAR and WASp nucleation‐promoting factors act sequentially to mediate Drosophila myoblast fusion

  2009cited by this paper
• Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages

  2009cited by this paper
• The immunoglobulin superfamily member Hbs functions redundantly with Sns in interactions between founder and fusion-competent myoblasts

  2009cited by this paper
• FuRMAS: triggering myoblast fusion in Drosophila

  2009cited by this paper
• Mechanics of membrane fusion

  2008cited by this paper
• STEM tomography for thick biological specimens.

  2008cited by this paper
• Drosophila ELMO/CED-12 interacts with Myoblast city to direct myoblast fusion and ommatidial organization.

  2008cited by this paper
• Viral and Developmental Cell Fusion Mechanisms: Conservation and Divergence

  2008cited by this paper
• Fusion-pore expansion during syncytium formation is restricted by an actin network

  2008cited by this paper
• Myoblast Fusion in Fly and Vertebrates: New Genes, New Processes and New Perspectives

  2008cited by this paper
• Mechanisms of membrane fusion: disparate players and common principles

  2008cited by this paper
• Myoblast fusion in Drosophila.

  2008cited by this paper
• The combination of chemical fixation procedures with high pressure freezing and freeze substitution preserves highly labile tissue ultrastructure for electron tomography applications.

  2008cited by this paper
• The atypical Rac activator Dock180 (Dock1) regulates myoblast fusion in vivo

  2008cited by this paper
• WASP and SCAR have distinct roles in activating the Arp2/3 complex during myoblast fusion

  2008cited by this paper
• A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila

  2007cited by this paper
• WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila.

  2007cited by this paper
• Myoblast fusion in Drosophila melanogaster is mediated through a fusion‐restricted myogenic‐adhesive structure (FuRMAS)

  2007cited by this paper
• SCAR/WAVE and Arp2/3 are crucial for cytoskeletal remodeling at the site of myoblast fusion

  2007cited by this paper
• A critical function for the actin cytoskeleton in targeted exocytosis of prefusion vesicles during myoblast fusion.

  2007cited by this paper
• The MARVEL domain protein, Singles Bar, is required for progression past the pre-fusion complex stage of myoblast fusion.

  2007cited by this paper
• Insect flight.

  2006cited by this paper
• Notch pathway repression by vestigial is required to promote indirect flight muscle differentiation in Drosophila melanogaster.

  2006cited by this paper
• Membranes of the world unite!

  2006cited by this paper
• High-pressure freezing, cellular tomography, and structural cell biology.

  2006cited by this paper
• The ARP2/3 complex: an actin nucleator comes of age

  2006cited by this paper
• Metamorphosis and the Formation of the Adult Musculature

  2006cited by this paper
• Automated electron microscope tomography using robust prediction of specimen movements.

  2005cited by this paper
• kette and blown fuse interact genetically during the second fusion step of myogenesis in Drosophila

  2004cited by this paper
• SNS: Adhesive properties, localization requirements and ectodomain dependence in S2 cells and embryonic myoblasts.

  2004cited by this paper
• Towards a molecular pathway for myoblast fusion in Drosophila.

  2004cited by this paper
• Genetic mosaic techniques for studying Drosophila development

  2003cited by this paper
• Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples

  2003cited by this paper
• Myoblast fusion in Drosophila.

  2002cited by this paper
• MARVEL: a conserved domain involved in membrane apposition events.

  2002cited by this paper
• Unconventional Rac-GEF activity is mediated through the Dock180–ELMO complex

  2002cited by this paper
• Freeze substitution of high‐pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water

  2002cited by this paper
• Wasp, the Drosophila Wiskott-Aldrich Syndrome Gene Homologue, Is Required for Cell Fate Decisions Mediated by Notch Signaling

  2001cited by this paper
• Fusomorphogenesis: Cell fusion in organ formation

  2000cited by this paper
• Drosophila dumbfounded: a myoblast attractant essential for fusion.

  2000cited by this paper
• Muscle pattern diversification in Drosophila: the story of imaginal myogenesis

  1999cited by this paper
• Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis.

  1999cited by this paper
• Development of the adult neuromuscular system.

  1999cited by this paper
• Patterning Muscles Using Organizers: Larval Muscle Templates and Adult Myoblasts Actively Interact to Pattern the Dorsal Longitudinal Flight Muscles of Drosophila

  1998cited by this paper
• Genetic Analysis of Myoblast Fusion: blown fuse Is Required for Progression Beyond the Prefusion Complex

  1997influential reference
• Computer visualization of three-dimensional image data using IMOD.

  1996cited by this paper
• Wingless signaling induces nautilus expression in the ventral mesoderm of the Drosophila embryo.

  1996cited by this paper
• Molecular genetic analysis of muscle development, structure, and function in Drosophila.

  1993cited by this paper
• Ultrastructure of developing flight muscle in Drosophila. II. Formation of the myotendon junction.

  1993cited by this paper
• Ultrastructure of developing flight muscle in Drosophila. I. Assembly of myofibrils.

  1993cited by this paper
• Development of the indirect flight muscles of Drosophila.

  1991cited by this paper
• Insect Flight

  1986cited by this paper
• ELECTRON MICROSCOPIC STUDIES ON THE INDIRECT FLIGHT MUSCLES OF DROSOPHILA MELANOGASTER

  1963cited by this paper
• ELECTRON MICROSCOPIC STUDIES ON THE INDIRECT FLIGHT MUSCLES OFDROSOPHILAMELANOGASTER

  1963cited by this paper

• Fusion of myofibre branches is a physiological feature of healthy human skeletal muscle regeneration

  2023cites this paper
• Contractile and expansive actin networks in Drosophila: Developmental cell biology controlled by network polarization and higher-order interactions.

  2023cites this paper
• Drosophila melanogaster: A Model System to Study Distinct Genetic Programs in Myoblast Fusion

  2022cites this paper
• Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations.

  2022cites this paper
• Programmed cell fusion in development and homeostasis.

  2021cites this paper
• Development of the indirect flight muscles of Aedes aegypti, a main arbovirus vector

  2021cites this paper
• YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion

  2021cites this paper
• Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion.

  2021influential citation
• Microtubules provide guidance cues for myofibril and sarcomere assembly and growth

  2020cites this paper
• Metamorphosis in Insect Muscle: Insights for Engineering Muscle-Based Actuators

  2020cites this paper
• Heterogeneity and Actin Cytoskeleton in Osteoclast and Macrophage Multinucleation

  2020cites this paper
• Muscle development : a view from adult myogenesis in Drosophila.

  2020cites this paper
• The fusogenic synapse at a glance

  2019cites this paper
• How cells fuse

  2019cites this paper
• Drosophila Myoblast Fusion: Invasion and Resistance for the Ultimate Union.

  2019cites this paper
• Quantitative basis of meiotic chromosome synapsis analyzed by electron tomography

  2019cites this paper
• Electron tomography of mouse LINC complexes at meiotic telomere attachment sites with and without microtubules

  2019cites this paper
• Cell Fusion: Merging Membranes and Making Muscle.

  2019cites this paper
• News in fusion

  2019cites this paper
• Myoblast fusion confusion: the resolution begins

  2018cites this paper
• EM Tomography of Meiotic LINC Complexes.

  2018cites this paper
• Srf controls satellite cell fusion through the maintenance of actin architecture

  2018cites this paper
• Live Imaging of Myogenesis in Indirect Flight Muscles in Drosophila.

  2017cites this paper
• Drosophila adult muscle development and regeneration.

  2017cites this paper
• Acting on identity: Myoblast fusion and the formation of the syncytial muscle fiber.

  2017cites this paper
• Skeletal muscle regeneration involves macrophage-myoblast bonding

  2017cites this paper
• The hallmarks of cell-cell fusion

  2017cites this paper
• Structure–function analysis of myomaker domains required for myoblast fusion

  2016cites this paper
• Identification and functional characterization of muscle satellite cells in Drosophila

  2016cites this paper
• WHAMY is a novel actin polymerase promoting myoblast fusion, macrophage cell motility and sensory organ development in Drosophila

  2016cites this paper
• Newly identified satellite cells respond to damage through Notch- Delta signaling to fuse with adult Drosophila muscles.

  2016cites this paper
• Myoblast fusion: Experimental systems and cellular mechanisms.

  2016cites this paper
• Metamorphosis of the Drosophila visceral musculature and its role in intestinal morphogenesis and stem cell formation.

  2016cites this paper
• Drosophila Kette coordinates myoblast junction dissolution and the ratio of Scar-to-WASp during myoblast fusion

  2016cites this paper
• Growing up and growing pores in myoblast fusion

  2015cites this paper