Visible/near-infrared subdiffraction imaging reveals the stochastic nature of DNA walkers

Super-resolution imaging reveals the stochastic behavior of DNA walkers. DNA walkers are designed with the structural specificity and functional diversity of oligonucleotides to actively convert chemical energy into mechanical translocation. Compared to natural protein motors, DNA walkers’ small translocation distance (mostly <100 nm) and slow reaction rate (<0.1 nm s−1) make single-molecule characterization of their kinetics elusive. An important indication of single-walker kinetics is the rate-limiting reactions that a particular walker design bears. We introduce an integrated super-resolved fluorescence microscopy approach that is capable of long-term imaging to investigate the stochastic behavior of DNA walkers. Subdiffraction tracking and imaging in the visible and second near-infrared spectra resolve walker structure and reaction rates. The distributions of walker kinetics are analyzed using a stochastic model to reveal reaction randomness and the rate-limiting biochemical reaction steps.

• Publication year

  2017

• Venue

  Science Advances

• Publication date

  2017-01-01

• Fields of study

  Biology, Medicine, Materials Science, Physics

• Identifiers
  DOI 10.1126/sciadv.1601600PMID 28116353PMCID 5249260
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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