Selective effects of acute and chronic ethanol exposure on neuropeptide and guanine nucleotide stimulated phospholipase C activity in intact N1E-115 neuroblastoma.

The influence of acute and chronic ethanol exposures on the coupling of neurotensin and bradykinin receptors to phospholipase C was determined in intact N1E-115 cells. Phospholipase C was monitored by the formation of total [3H]inositol phosphates in the presence of lithium in cells prelabeled with [3H]inositol. Acute exposure to ethanol over a range of 50 to 200 mM inhibited the stimulation of [3H]inositol phosphate formation elicited by neurotensin and bradykinin. In cells chronically exposed to 100 mM ethanol for 7 days, neither basal- nor neurotensin-stimulated [3H]inositol phosphate formation differed significantly from those of control (untreated) cells. In contrast, the [3H]inositol response to bradykinin was significantly inhibited in cells chronically exposed to ethanol. Because chronic ethanol exposure had no parallel effects on either the specific binding of [3H]bradykinin or the stimulation of [3H]inositol phosphate formation by the stable GTP analog, guanine 5'-(y-thiotriphosphate), it is suggested that chronic ethanol impairs the ability of bradykinin receptors to activate the guanine nucleotide binding protein associated with phospholipase C. In addition, because chronic ethanol had no effect on the inositol phosphate response to neurotensin, it is proposed that certain types of receptor-guanine nucleotide binding protein interactions are more vulnerable than are others to disruption by chronic ethanol treatment.

• Publication year

  1991

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1991-08-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)20483-9PMID 1907659
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ethanol effects on two types of voltage-activated calcium channels.

  1990cited by this paper
• Angiotensin II Effects on the Cytosolic Free Ca2+ Concentration in N1E‐115 Neuroblastoma Cells: Kinetic Properties of the Ca2+ Transient Measured in Single Fura‐2‐Loaded Cells

  1990cited by this paper
• Ethanol and guanine nucleotide binding proteins: a selective interaction

  1990cited by this paper
• Guanine nucleotide binding proteins in neuroblastoma x glioma hybrid, NG108-15, cells. Regulation of expression and function.

  1990cited by this paper
• Functionally distinct G proteins selectively couple different receptors to PI hydrolysis in the same cell.

  1989cited by this paper
• Effects of ethanol on muscarinic receptor-stimulated phosphoinositide metabolism during brain development.

  1989cited by this paper
• Effects of bradykinin, GTP gamma S, R59022 and N-ethylmaleimide on inositol phosphate production in NG108-15 cells.

  1989cited by this paper
• Bradykinin effects on phospholipid metabolism and its relation to arachidonic acid turnover in neuroblastoma x glioma hybrid cells (NG 108-15).

  1989cited by this paper
• Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells.

  1989cited by this paper
• Two different G-proteins mediate neuropeptide Y and bradykinin-stimulated phospholipid breakdown in cultured rat sensory neurons.

  1989cited by this paper
• Inositol phosphates and cell signalling

  1989cited by this paper
• The receptors for ATP and fMetLeuPhe are independently coupled to phospholipases C and A2 via G-protein(s). Relationship between phospholipase C and A2 activation and exocytosis in HL60 cells and human neutrophils.

  1989cited by this paper
• Role of a protein regulating guanine nucleotide binding in phosphoinositide breakdown and calcium mobilization by bradykinin in neuroblastoma X glioma hybrid NG108-15 cells: effects of pertussis toxin and cholera toxin on receptor-mediated signal transduction.

  1987cited by this paper
• Characterization of two types of calcium channels in mouse neuroblastoma cells.

  1987cited by this paper
• Stimulation of Inositol Phosphate Production by Neurotensin in Neuroblastoma N1E115 Cells: Implication of GTP‐Binding Proteins and Relationship with the Cyclic GMP Response

  1987cited by this paper
• Competitive inhibition of phosphoinositide hydrolysis by the muscarinic receptor antagonist AF-DX 116 is of low affinity in mouse cerebral cortex.

  1987cited by this paper
• Effect of ethanol on receptor-stimulated phosphatidic acid and polyphosphoinositide metabolism in mouse brain.

  1986cited by this paper
• Effects of ethanol on stimulated inositol phospholipid hydrolysis in rat brain.

  1986cited by this paper
• Neurotensin-mediated inhibition of cyclic AMP formation in neuroblastoma N1E115 cells: involvement of the inhibitory GTP-binding component of adenylate cyclase.

  1986cited by this paper
• Acute and chronic effects of ethanol on receptor-mediated phosphatidylinositol 4,5-bisphosphate breakdown in mouse brain.

  1986cited by this paper
• Neurotensin Stimulates Inositol Phospholipid Metabolism and Calcium Mobilization in Murine Neuroblastoma Clone N1E‐115

  1986cited by this paper
• Effects of pertussis toxin on cAMP and cGMP responses to carbamylcholine in N1E-115 neuroblastoma cells.

  1985cited by this paper
• Bradykinin Receptor‐Mediated Cyclic GMP Formation in a Nerve Cell Population (Murine Neuroblastoma Clone N1E‐115)

  1984cited by this paper
• Bradykinin-induced rapid breakdown of phosphatidylinositol 4,5-bisphosphate in neuroblastoma X glioma hybrid NG108-15 cells.

  1984cited by this paper
• Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands.

  1982cited by this paper
• Regulation of Muscarinic Receptor‐Mediated Cyclic GMP Synthesis by Cultured Mouse Neuroblastoma Cells

  1980cited by this paper
• Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture.

  1976cited by this paper
• Responses of neuroblastoma cells to iontophoretically applied acetylcholine

  1971cited by this paper

• The role of G proteins in the activity and ethanol modulation of glycine-induced currents in rat neurons freshly isolated from the ventral tegmental area.

  2005cites this paper
• LF 16-0687 Ms, a new bradykinin B2 receptor antagonist, improves neurologic outcome but not brain tissue prostaglandin E2 release in a rat model of closed head trauma combined with ethanol intoxication.

  2003cites this paper
• Phosphatidic acid modulates G protein regulation of phospholipase C-beta1 activity in membranes.

  2002cites this paper
• Chronic ethanol exposure enhances signaling through muscarinic receptors expressed by cRNA injection in Xenopus oocytes: implications for mechanism of action.

  1999cites this paper
• Acute ethanol exposure attenuates expression of junD in human neuroblastoma cells.

  1998cites this paper
• The effect of ethanol on muscarinic receptor-G protein coupling in the rat cortex.

  1997cites this paper
• Long-term exposure to ethanol increases the number and function of muscarinic M1 receptors in human neuroblastoma cells.

  1996cites this paper
• Potentiation of the glycine-activated Cl- current by ethanol in cultured mouse spinal neurons.

  1996cites this paper
• Lack of involvement of protein kinase C in ethanol-induced inhibition of metabotropic-glutamate receptor function in primary cultures of astrocytes.

  1995cites this paper
• Molecular and cellular adaptations in signal transduction pathways following ethanol exposure.

  1995cites this paper
• Ethanol inhibits the peak of muscarinic receptor-stimulated formation of inositol 1,4,5-trisphosphate in neuroblastoma SH-SY5Y cells.

  1995cites this paper
• Adaptation of signal transduction in brain.

  1994cites this paper
• Ethanol inhibits the function of 5-hydroxytryptamine type 1c and muscarinic M1 G protein-linked receptors in Xenopus oocytes expressing brain mRNA: role of protein kinase C.

  1994cites this paper
• Fetal alcohol syndrome: the vulnerability of the developing brain and possible mechanisms of damage

  1994cites this paper
• Chronic ethanol exposure inhibits ATP-stimulated but not KCl-stimulated dopamine release in PC 12 cells.

  1994cites this paper
• Selective effects of ethanol exposure on metabotropic glutamate receptor and guanine nucleotide stimulated phospholipase C activity in primary cultures of astrocytes.

  1994cites this paper
• Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function.

  1993cites this paper
• Effects of ethanol exposure on neuropeptide-stimulated calcium mobilization in N1E-115 neuroblastoma.

  1993cites this paper
• Characterization of neurotensin-stimulated phosphoinositide hydrolysis in brain regions of long sleep and short sleep mice.

  1993cites this paper
• Selective Effects of Ethanol on Neuropeptide — Mediated Polyphosphoinositide Hydrolysis and Calcium Mobilization

  1993cites this paper
• Differential effects of chronic ethanol exposure on ATP- and bradykinin-induced increases in intracellular calcium levels in PC-12 cells.

  1993cites this paper