Regulation of cry Gene Expression in Bacillus thuringiensis

Bacillus thuringiensis differs from the closely related Bacillus cereus group species by its ability to produce crystalline inclusions. The production of these crystals mainly results from the expression of the cry genes, from the stability of their transcripts and from the synthesis, accumulation and crystallization of large amounts of insecticidal Cry proteins. This process normally coincides with sporulation and is regulated by various factors operating at the transcriptional, post-transcriptional, metabolic and post-translational levels.

• Publication year

  2014

• Venue

  Toxins

• Publication date

  2014-07-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/toxins6072194PMID 25055802PMCID 4113751
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Division of labour and terminal differentiation in a novel Bacillus thuringiensis strain

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  2013influential reference
• Overexpression of polyphosphate kinase gene (ppk) increases bioinsecticide production by Bacillus thuringiensis.

  2013influential reference
• Cooperation and the evolutionary ecology of bacterial virulence: The Bacillus cereus group as a novel study system

  2013cited by this paper
• Expression profile and regulation of spore and parasporal crystal formation-associated genes in Bacillus thuringiensis.

  2013cited by this paper
• Identification of the Promoter in the Intergenic Region between orf1 and cry8Ea1 Controlled by Sigma H Factor

  2012cited by this paper
• The Dynamics of Cooperative Bacterial Virulence in the Field

  2012cited by this paper
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  2012cited by this paper
• Gene Clusters Located on Two Large Plasmids Determine Spore Crystal Association (SCA) in Bacillus thuringiensis Subsp. finitimus Strain YBT-020

  2011influential reference
• Bacillus thuringiensis: A story of a successful bioinsecticide.

  2011cited by this paper
• Bacillus thuringiensis: a century of research, development and commercial applications.

  2011cited by this paper
• Potential effect of a putative sigma(H)-driven promoter on the over expression of the Cry1Ac toxin of Bacillus thuringiensis.

  2010cited by this paper
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• A novel negative regulatory factor for nematicidal Cry protein gene expression in Bacillus thuringiensis.

  2008influential reference
• Biology and taxonomy of Bacillus cereus, Bacillus anthracis, and Bacillus thuringiensis.

  2007cited by this paper
• The 20-kDa Protein of Bacillus thuringiensis subsp. israelensis Enhances Bacillus sphaericus 2362 Bin Toxin Synthesis

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• [Effects of helper protein P20 from Bacillus thuringiensis on Vip3A expression].

  2006cited by this paper
• Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests

  2006cited by this paper
• Inorganic phosphate has a crucial effect on Cry3Aa δ‐endotoxin production

  2005cited by this paper
• Sporulation of Bacillus subtilis.

  2004cited by this paper
• Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein

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• Nutritional and cultural parameters influencing antidipteran delta-endotoxin production.

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• The Spo0A regulon of Bacillus subtilis

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• Structure, diversity, and evolution of protein toxins from spore-forming entomopathogenic bacteria.

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• Genome structure and evolution of the Bacillus cereus group.

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• Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.

  2002influential reference
• Effects of the 20-Kilodalton Helper Protein on Cry1Ac Production and Spore Formation in Bacillus thuringiensis

  2001influential reference
• Cellular Localization and Characterization of the Bacillus thuringiensis Orf2 Crystallization Factor

  2001influential reference
• Regulation of the Acetoin Catabolic Pathway Is Controlled by Sigma L in Bacillus subtilis

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• Inorganic phosphate regulates CryIVA protoxin expression in Bacillus thuringiensis israelensis.

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• Bacillus popilliae cry18Aa operon is transcribed by σE and σK forms of RNA polymerase from a single initiation site

  1998cited by this paper
• Modulation of Cry IV A toxin protein expression by glucose in Bacillus thuringiensis israelensis.

  1998influential reference
• Differential effects of helper proteins encoded by the cry2A and cry11A operons on the formation of Cry2A inclusions in Bacillus thuringiensis.

  1998influential reference
• Optimization of Cry3A Yields in Bacillus thuringiensis by Use of Sporulation-Dependent Promoters in Combination with the STAB-SD mRNA Sequence

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• Binding of the Catabolite Repressor Protein CcpA to Its DNA Target Is Regulated by Phosphorylation of its Corepressor HPr*

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• 2-Oxo acid dehydrogenase multienzyme complexes. The central role of the lipoyl domain.

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• Spo0A represses transcription of the cry toxin genes in Bacillus thuringiensis.

  1997influential reference
• Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins.

  1996cited by this paper
• STAB‐SD: a Shine–Dalgarno sequence in the 5′ untranslated region is a determinant of mRNA stability

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• Overproduction of Encapsulated Insecticidal Crystal Proteins in a Bacillus thuringiensis spoOA Mutant

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• Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis

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• Identification of a positive retroregulator that stabilizes mRNAs in bacteria.

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• Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNA.

  1972cited by this paper

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