Characterization and influence of hydroxyapatite nanopowders on living cells

Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and respiratory systems.

• Publication year

  2018

• Venue

  Beilstein Journal of Nanotechnology

• Publication date

  2018-12-27

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.3762/bjnano.9.286PMID 30643706PMCID 6317412
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Structure and physico-mechanical properties of low temperature plasma treated electrospun nanofibrous scaffolds examined with atomic force microscopy.

  2018cited by this paper
• Multi-scale characterization and biological evaluation of composite surface layers produced under glow discharge conditions on NiTi shape memory alloy for potential cardiological application.

  2018cited by this paper
• Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells

  2017cited by this paper
• Modified Pechini synthesis of Bi2ZnB2O7 nanoparticles

  2017cited by this paper
• Microstructure and nanomechanical properties of single stalks from diatom Didymosphenia geminata and their change due to adsorption of selected metal ions

  2017cited by this paper
• Microscopic Methods for Characterization of Selected Surface Properties of Biodegradable, Nanofibrous Tissue Engineering Scaffolds

  2017cited by this paper
• Nanoengineered biocomposite tricomponent polymer based matrices for bone tissue engineering

  2016cited by this paper
• Influence of hydrothermal synthesis parameters on the properties of hydroxyapatite nanoparticles

  2016cited by this paper
• How important are scaffolds and their surface properties in regenerative medicine

  2016cited by this paper
• Overview of Workplace Exposure to Nanomaterials

  2015cited by this paper
• Reducing bacteria and macrophage density on nanophase hydroxyapatite coated onto titanium surfaces without releasing pharmaceutical agents.

  2015cited by this paper
• Poster Presentations

  2014cited by this paper
• Calcium phosphate-bearing matrices induce osteogenic differentiation of stem cells through adenosine signaling

  2014cited by this paper
• Synthesis and Characterization of Nano-hydroxyapatite Powder Using Wet Chemical Precipitation Reaction

  2014cited by this paper
• Differential cytotoxicity and particle action of hydroxyapatite nanoparticles in human cancer cells.

  2014cited by this paper
• Effect of single-wall carbon nanotubes on mechanical property of chondrocytes.

  2014cited by this paper
• Highly biocompatible, nanocrystalline hydroxyapatite synthesized in a solvothermal process driven by high energy density microwave radiation

  2013cited by this paper
• Nanosheet-assembled hierarchical nanostructures of hydroxyapatite: surfactant-free microwave-hydrothermal rapid synthesis, protein/DNA adsorption and pH-controlled release

  2013cited by this paper
• Comprehensive structural studies of ultra-fine nanocrystalline calcium hydroxyapatite using MAS NMR and FT-IR spectroscopic methods

  2013cited by this paper
• Nano-bio effects: interaction of nanomaterials with cells.

  2013cited by this paper
• Synthesis and characterization of lithium calcium phosphate ceramics

  2013cited by this paper
• Novel Synthesis Method and Biomedical Applications of Doped and Undoped Hydroxyapatites and Fluoroapatites

  2013cited by this paper
• Statistical evaluation of toxicological assays: Dunnett or Williams test—take both

  2013cited by this paper
• Hydroxylapatite nanoparticles: fabrication methods and medical applications

  2012cited by this paper
• Comparison of Hydrothermal and Sol-Gel Synthesis of Nano-Particulate Hydroxyapatite by Characterisation at the Bulk and Particle Level

  2012cited by this paper
• Cytotoxicity of hydroxyapatite nanoparticles is shape and cell dependent

  2012cited by this paper
• Dissolution mechanism of calcium apatites in acids: A review of literature.

  2012cited by this paper
• Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7)

  2012cited by this paper
• Effects of four types of hydroxyapatite nanoparticles with different nanocrystal morphologies and sizes on apoptosis in rat osteoblasts

  2012cited by this paper
• Hydroxyapatite-Based Materials: Synthesis and Characterization

  2011cited by this paper
• The role of surface charge on the uptake and biocompatibility of hydroxyapatite nanoparticles with osteoblast cells

  2011cited by this paper
• p53 reaction to apoptosis induced by hydroxyapatite nanoparticles in rat macrophages.

  2009cited by this paper
• Hydroxyapatite nano and microparticles: correlation of particle properties with cytotoxicity and biostability.

  2009cited by this paper
• Mitochondrial calcium as a key regulator of mitochondrial ATP production in mammalian cells.

  2009cited by this paper
• Regulation of mitochondrial dehydrogenases by calcium ions.

  2009cited by this paper
• The importance of particle size and DNA condensation salt for calcium phosphate nanoparticle transfection.

  2008cited by this paper
• Calcium signaling.

  2007cited by this paper
• The costs of using unauthenticated, over-passaged cell lines: how much more data do we need?

  2007cited by this paper
• Calcium Signaling

  2007cited by this paper
• Nanomaterials and nanoparticles: Sources and toxicity

  2007cited by this paper
• Nanotechnology and health safety--toxicity and risk assessments of nanostructured materials on human health.

  2007cited by this paper
• Bone cell transfection in tissue culture using hydroxyapatite microparticles.

  2006cited by this paper
• Safe handling of nanotechnology

  2006cited by this paper
• Quantitative methods for nanopowders characterization

  2006cited by this paper
• Calcium at fertilization and in early development.

  2006cited by this paper
• TIGAR, a p53-inducible regulator of glycolysis and apoptosis.

  2006cited by this paper
• Assessing the risks of manufactured nanomaterials.

  2006cited by this paper
• Cellular toxicity of carbon-based nanomaterials.

  2006cited by this paper
• The Change of Intracellular pH is Involved in the Cisplatin-Resistance of Human Lung Adenocarcinoma A549/DDP Cells

  2005cited by this paper
• Nanocrystalline hydroxyapatite: micelle templated synthesis and characterization.

  2005cited by this paper
• Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy

  2005cited by this paper
• Morphology Control of Materials and Nanoparticles

  2004cited by this paper
• The International Organization for Standardization.

  2003cited by this paper
• Hydroxyapatite and Hydroxyapatite-Based Ceramics

  2002cited by this paper
• Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods.

  2002cited by this paper
• Calcium signalling--an overview.

  2001cited by this paper
• Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids

  2000cited by this paper
• The kinetics of pentoxifylline release from drug-loaded hydroxyapatite implants.

  2000cited by this paper
• Regulation of p53 Function and Stability by Phosphorylation

  1999cited by this paper
• DNA damage activates p53 through a phosphorylation-acetylation cascade.

  1998cited by this paper
• Plasma sprayed hydroxyapatite coatings on titanium substrates. Part 1: Mechanical properties and residual stress levels.

  1998cited by this paper
• The role of calcium in cell division.

  1994cited by this paper
• Calcium ions and oxidative cell injury

  1992cited by this paper
• The role of macrophages in immunology.

  1983cited by this paper
• The crystallization of hydroxyapatite and dicalcium phosphate dihydrate; representation of growth curves

  1982cited by this paper
• Hydroxyapatite phagocytosis by human polymorphonuclear leucocytes.

  1979cited by this paper
• The anomalous behaviour of the neutron reflexion of fluorite

  1965cited by this paper
• Synthetic hydroxyapatite crystals. 1. Sodium and potassium fixation.

  1962cited by this paper

• Encapsulation of Syrah red wine polyphenols by nano spray dryer

  2026cites this paper
• Empirical quantification of bone mineral and organic phase attenuation coefficients using CT imaging and controlled thermal processing.

  2025cites this paper
• Environmentally sustainable green synthesis of novel nano-hydroxyapatite derived from Sesbania grandiflora leaf and Sesamum indicum seed: In Vitro evaluation for dental restorative applications

  2025cites this paper
• In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite–Polylactic Acid Scaffolds Intended for Bone Regeneration

  2025cites this paper
• Magnetically driven engineering of magnetic hydroxyapatite/CA RO membranes: Enhancing surface physicochemical properties for high-performance water desalination

  2025cites this paper
• Recovery of calcium from maize Lime-Cooking wastewater as hydroxyapatite for biomedical applications

  2025cites this paper
• A comparative X-ray diffraction analysis of Sr2+substituted hydroxyapatite from sand lobster shell waste using various methods

  2025cites this paper
• An assessment of microstructure, dentinal tubule occlusion and X-ray attenuation properties of Nd:YAG laser-enhanced titanium-doped phosphate glass and nano-hydroxyapatite pastes

  2024influential citation
• A Brief Study of electrical and biological properties of BNT6BT/ZnO-HA Composite

  2024cites this paper
• Mechanical hydroxyapatite coatings on PEO-treated Ti–6Al–4V alloy for enhancing implant's surface bioactivity

  2024cites this paper
• Quantitative Imaging of Magnesium Biodegradation by 3D X‐Ray Ptychography and Electron Microscopy

  2024cites this paper
• Development and Optimization of Nano-Hydroxyapatite Encapsulating Tocotrienol-Rich Fraction Formulation Using Response Surface Methodology

  2024cites this paper
• TiO2 coatings doped with MoS2 nanoparticles using plasma electrolytic oxidation on Ti-6Al-4V alloy: Application for enhanced and functional bio-implant surface

  2024cites this paper
• Nano-scale modeling of energy dissipation in a single lamella reveals the significant contribution of collagen-mineral sliding to intrinsic bone toughness

  2024influential citation
• Radiological properties of nano-hydroxyapatite compared to natural equine hydroxyapatite quantified using dual-energy CT and high-field MR.

  2024cites this paper
• Antibacterial activity of microwave synthesized hydroxyapatite against cariogenic bacteria: A preliminary study

  2024cites this paper
• Multifunctional 3D-printed bioceramic scaffolds: Recent strategies for osteosarcoma treatment

  2023cites this paper
• Aluminum adsorption using different models of hydroxyapatite via Molecular Dynamic simulations

  2023cites this paper
• Metal Ion-Doped Hydroxyapatite-Based Materials for Bone Defect Restoration

  2023cites this paper
• Osteogenic Properties of Titanium Alloy Ti6Al4V-Hydroxyapatite Composites Fabricated by Selective Laser Melting

  2023cites this paper
• A comparative study of the mechanical properties of sol-gel derived hydroxyapatite produced from a novel mixture of two natural biowastes for biomedical applications

  2023cites this paper
• Flake Graphene-Based Nanomaterial Approach for Triggering a Ferroptosis as an Attractive Theranostic Outlook for Tackling Non-Small Lung Cancer: A Mini Review

  2022cites this paper
• Metformin-Incorporated Gelatin/Nano-Hydroxyapatite Scaffolds Promotes Bone Regeneration in Critical Size Rat Alveolar Bone Defect Model

  2022cites this paper
• Adsorptive removal of Acid Blue 113 using hydroxyapatite nanoadsorbents synthesized using Peltophorum pterocarpum pod extract.

  2022cites this paper
• Poly(N-isopropylacrylamide)-Based Hydrogels for Biomedical Applications: A Review of the State-of-the-Art

  2022cites this paper
• Assessment of crystallite size of UV-synthesized hydroxyapatite using different model equations

  2022cites this paper
• Short and long-term effects of nanobiomaterials in fish cell lines. Applicability of RTgill-W1.

  2022cites this paper
• PRODUÇÃO DE HIDROXIAPATITA DE CÁLCIO PARA USO EM VOLUMIZAÇÃO FACIAL E BIOESTIMULAÇÃO DE COLÁGENO

  2022cites this paper
• A multi-functionalized calcitriol sustainable delivery system for promoting osteoporotic bone regeneration both in vitro and in vivo

  2021cites this paper
• on Hydroxyapatite and Eggshell Ostrich Micro of Study Comparative Calvarium Rat the in Created Defect Bone of Healing the

  2021cites this paper
• Synergistic effects of wheat gluten and poly (γ-glutamic acid) on physicochemical properties of bioglass modified beta-tricalcium phosphate bone cement

  2021cites this paper
• An Occam’s razor: Synthesis of osteoinductive nanocrystalline implant coatings on hierarchical superstructures formed by Mugil cephalus skin hydrolysate

  2021cites this paper
• Preparation of magnetic methotrexate nanocarrier coated with extracted hydroxyapatite of sea urchin (Echinometra mathaei)

  2021cites this paper
• Bioceramics in Tissue Engineering: Retrospect and Prospects

  2021cites this paper
• Fabrication and applications of bioactive chitosan-based organic-inorganic hybrid materials: A review.

  2021cites this paper
• Emerging marine derived nanohydroxyapatite and their composites for implant and biomedical applications.

  2021cites this paper
• To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with Bletilla striata Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration

  2021cites this paper
• Characterization and evaluation of porous hydroxyapatite synthesized by oil-in-water method as carrier of donepezil for the preventive of Alzheimer’s disease by controlled release

  2020cites this paper
• AFM force spectroscopy as a powerful tool to address material design for biomedical applications. A review

  2020cites this paper
• Dual-functional scaffolds of poly(L-lactic acid)/nanohydroxyapatite encapsulated with metformin: Simultaneous enhancement of bone repair and bone tumor inhibition.

  2020cites this paper
• Hybrid bioactive hydroxyapatite/polycaprolactone nanoparticles for enhanced osteogenesis.

  2020cites this paper
• Ecofriendly production of bioactive tissue engineering scaffolds derived from egg- and sea-shells

  2020cites this paper
• Curcumin-encased hydroxyapatite nanoparticles as novel biomaterials for antimicrobial, antioxidant and anticancer applications: A perspective of nano-based drug delivery

  2020cites this paper
• Calcium phosphate stability on melt electrowritten PCL scaffolds

  2020influential citation
• Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

  2020cites this paper
• In vivo and in vitro study of a novel nanohydroxyapatite sonocoated scaffolds for enhanced bone regeneration.

  2019cites this paper
• Internal nanocrystalline structure and stiffness alterations of electrospun polycaprolactone-based mats after six months of in vitro degradation. An atomic force microscopy assay.

  2019cites this paper
• Porous plasma sprayed bioceramic (Ca10 (PO4).6(OH)2) coated Ti6Al4V: morphological, adhesion and tribological studies

  2019cites this paper
• How reversible are the effects of silver nanoparticles on macrophages? A proteomic-instructed view

  2019cites this paper
• Nanohydroxyapatite adhesion to low temperature plasma modified surface of 3D-printed bone tissue engineering scaffolds - qualitative and quantitative study

  2019cites this paper
• Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration

  2019cites this paper
• Polymer Membranes Sonocoated and Electrosprayed with Nano-Hydroxyapatite for Periodontal Tissues Regeneration

  2019cites this paper
• Synthesis and Characterization of Hydroxyapatite for Mercury Removal in Polluted Waters

  2019cites this paper