Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.

• Publication year

  2012

• Venue

  Plant Methods

• Publication date

  2012-05-06

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1186/1746-4811-8-15PMID 22559714PMCID 3475070
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A bicistronic, Ubiquitin-10 promoter-based vector cassette for transient transformation and functional analysis of membrane transport demonstrates the utility of quantitative voltage clamp studies on intact Arabidopsis root epidermis.

  2011cited by this paper
• AtALMT12 represents an R-type anion channel required for stomatal movement in Arabidopsis guard cells.

  2010cited by this paper
• Dynamic regulation of guard cell anion channels by cytosolic free Ca2+ concentration and protein phosphorylation.

  2010cited by this paper
• Closing Plant Stomata Requires a Homolog of an Aluminum-Activated Malate Transporter

  2010cited by this paper
• A Tripartite SNARE-K+ Channel Complex Mediates in Channel-Dependent K+ Nutrition in Arabidopsis[W]

  2009cited by this paper
• Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins

  2009influential reference
• Activity Determinants and Functional Specialization of Arabidopsis PEN1 Syntaxin in Innate Immunity*

  2008cited by this paper
• CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells

  2008cited by this paper
• SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling

  2008cited by this paper
• Abscisic acid triggers the endocytosis of the arabidopsis KAT1 K+ channel and its recycling to the plasma membrane.

  2007cited by this paper
• Membrane Transport and Ca2+ Oscillations in Guard Cells

  2007cited by this paper
• Tailor-made composite functions as tools in model choice: the case of sigmoidal vs bi-linear growth profiles

  2006cited by this paper
• The plant clock shows its metal: circadian regulation of cytosolic free Ca(2+).

  2005cited by this paper
• The Ascorbic Acid Redox State Controls Guard Cell Signaling and Stomatal Movement

  2004cited by this paper
• GUARD CELL SIGNAL TRANSDUCTION.

  2003cited by this paper
• Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acid-evoked signaling pathways

  2003cited by this paper
• The role of stomata in sensing and driving environmental change

  2003cited by this paper
• Selection pressures on stomatal evolution.

  2002cited by this paper
• Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status.

  2002cited by this paper
• A defined range of guard cell calcium oscillation parameters encodes stomatal movements

  2001cited by this paper
• Cellular signaling and volume control in stomatal movements in plants.

  2000cited by this paper
• Effect of cell-wall-digesting enzymes on physiological state and competence of maize coleoptile cells

  1999cited by this paper
• Advanced patch-clamp techniques and single-channel analysis

  1999cited by this paper
• A tobacco syntaxin with a role in hormonal control of guard cell ion channels.

  1999cited by this paper
• The membrane potential of Arabidopsis thaliana guard cells; depolarizations induced by apoplastic acidification

  1998cited by this paper
• Pharmacological properties of slow anion currents in intact guard cells of Arabidopsis. Application of the discontinuous single-electrode voltage-clamp to different species

  1998influential reference
• Alteration of anion channel kinetics in wild-type and abi1-1 transgenic Nicotiana benthamiana guard cells by abscisic acid.

  1997cited by this paper
• Ion channels in guard cells of Arabidopsis thaliana (L.) Heynh.

  1996cited by this paper
• NO3− transport across the plasma membrane of Arabidopsis thaliana root hairs: Kinetic control by pH and membrane voltage

  1995cited by this paper
• K+ channels of stomatal guard cells: Abscisic-acid-evoked control of the outward rectifier mediated by cytoplasmic pH

  1993cited by this paper
• Identification and characterization of a chlorate-resistant mutant of Arabidopsis thaliana with mutations in both nitrate reductase structural genes NIA1 and NIA2

  1993cited by this paper
• Voltage dependence of theChara proton pump revealed by current-voltage measurement during rapid metabolic blockade with cyanide

  1990cited by this paper
• Reversible inactivation of K+ channels of Vcia stomatal guard cells following the photolysis of caged inositol 1,4,5-trisphosphate

  1990cited by this paper
• Methods in plant biochemistry

  1989cited by this paper
• Electrical characteristics of stomatal guard cells: The ionic basis of the membrane potential and the consequence of potassium chlorides leakage from microelectrodes

  1987influential reference
• Electrical characteristics of stomatal guard cells: The contribution of ATP-dependent, “Electrogenic” transport revealed by current-voltage and difference-current-voltage analysis

  1987cited by this paper
• Role of "active" potassium transport in the regulation of cytoplasmic pH by nonanimal cells.

  1987cited by this paper
• Ultrastructural and histochemical studies on guard cells

  1984cited by this paper
• Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

  1981cited by this paper
• Single-channel currents recorded from membrane of denervated frog muscle fibres

  1976cited by this paper
• Electric current flow in excitable cells

  1975cited by this paper
• Membrane Transport in Plants

  1974cited by this paper
• Electrical coupling between cells of higher plants: A direct demonstration of intercellular communication

  1972cited by this paper
• An Algorithm for Least-Squares Estimation of Nonlinear Parameters

  1963cited by this paper

• The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration

  2024cites this paper
• Woody species grown under sun and shade present similar stomatal speed

  2023cites this paper
• Speedy stomata of a C4 plant correlate with enhanced K+ channel gating

  2023cites this paper
• Guard Cell Starch Degradation Yields Glucose for Rapid Stomatal Opening in Arabidopsis[CC-BY]

  2020influential citation
• A conserved chloroplast retrograde signal regulates stomatal closure for drought tolerance in plants

  2019influential citation
• Isolation of high purity guard cell protoplasts of Arabidopsis thaliana for omics research

  2019cites this paper
• Training Memory: Exploring the Intersection of Plant Stress Signalling and DNA Methylation

  2018cites this paper
• FINAL VERSION BEFORE PROOF Temporal dynamics of stomatal behaviour : modelling , and implications for photosynthesis and water use

  2017cites this paper
• Evolutionary Conservation of ABA Signaling for Stomatal Closure1[OPEN]

  2017cites this paper
• Temporal Dynamics of Stomatal Behavior: Modeling and Implications for Photosynthesis and Water Use1[OPEN]

  2017cites this paper
• Update on Temporal Dynamics of Stomatal Behavior Temporal Dynamics of Stomatal Behavior : Modeling and Implications for Photosynthesis and Water Use 1 [ OPEN ]

  2017cites this paper
• Nitrate reductase mutation alters potassium nutrition as well as nitric oxide-mediated control of guard cell ion channels in Arabidopsis.

  2016cites this paper
• Topical Review on Stomata and Water Use Ef fi ciency Stomatal Size , Speed , and Responsiveness Impact on Photosynthesis and Water Use Ef fi ciency 1 [ C ]

  2014cites this paper
• Voltage-Sensor Transitions of the Inward-Rectifying K+ Channel KAT1 Indicate a Latching Mechanism Biased by Hydration within the Voltage Sensor1[W][OPEN]

  2014cites this paper
• Clustering of the K+ channel GORK of Arabidopsis parallels its gating by extracellular K+

  2014cites this paper
• Analysis of gas exchange, stomatal behaviour and micronutrients uncovers dynamic response and adaptation of tomato plants to monochromatic light treatments.

  2014cites this paper
• Stomatal clustering and hydrogen sulfide: unraveling novel aspects of stomatal behavior

  2014influential citation
• Photosynthesis and Water Use Efficiency 1(C)

  2014cites this paper
• Stomatal Size, Speed, and Responsiveness Impact on Photosynthesis and Water Use Efficiency1[C]

  2014cites this paper
• Regulation of guard cell anion channels

  2013cites this paper
• PYR/PYL/RCAR Abscisic Acid Receptors Regulate K+ and Cl− Channels through Reactive Oxygen Species-Mediated Activation of Ca2+ Channels at the Plasma Membrane of Intact Arabidopsis Guard Cells1[W][OPEN]

  2013cites this paper
• Systems Dynamic Modeling of a Guard Cell Cl− Channel Mutant Uncovers an Emergent Homeostatic Network Regulating Stomatal Transpiration1[W][OA]

  2012influential citation