Enzyme improvement in the absence of structural knowledge: a novel statistical approach

No abstract is available for this paper.

• Publication year

  2008

• Venue

  The ISME Journal

• Publication date

  2008-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/ismej.2007.100PMID 18253133
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Design of active analogues of a 15-residue peptide using D-optimal design, QSAR and a combinatorial search algorithm.

  2009cited by this paper
• Improving catalytic function by ProSAR-driven enzyme evolution

  2007influential reference
• Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution

  2006cited by this paper
• Improving the thermal stability of lactate oxidase by directed evolution

  2006influential reference
• New enzyme for reductive cancer chemotherapy, YieF, and its improvement by directed evolution

  2006cited by this paper
• Fancy footwork in the sequence space shuffle

  2006cited by this paper
• Effect of Chromate Stress on Escherichia coli K-12

  2006cited by this paper
• Directed evolution of metabolic pathways.

  2006cited by this paper
• Design and Evolution of New Catalytic Activity with an Existing Protein Scaffold

  2006cited by this paper
• Improving the catalytic activity of Candida antarctica lipase B by circular permutation.

  2005cited by this paper
• Modeling and Analysis of Protein Design under Resource Constraints

  2005cited by this paper
• Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design.

  2005cited by this paper
• The 'evolvability' of promiscuous protein functions

  2005cited by this paper
• Chromate-Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia coli

  2004cited by this paper
• Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins.

  2004cited by this paper
• Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954.

  2003cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Antibiotic activity of pentadecapeptides modelled from amino acid descriptors

  2001cited by this paper
• Effect of double mutation on thermostability of lactate oxidase

  2000cited by this paper
• Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies.

  2000cited by this paper
• Adaptive walks by the fittest among finite random mutants on a Mt. Fuji-type fitness landscape II. Effect of small non-additivity

  2000cited by this paper
• Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase

  2000cited by this paper
• Spectrophotometric determination of uranium using 2-(2- Thiazolylazo)-p-Cresol (TAC) in the presence of surfactants

  1999cited by this paper
• Adaptive Walks by the Fittest among Finite Random Mutants on a Mt. Fuji-type Fitness Landscape.

  1998cited by this paper
• Enzyme engineering reaches the boiling point.

  1998cited by this paper
• Darwin’s Dangerous Idea: Evolution and the Meanings of Life

  1996cited by this paper
• Tolerance of different proteins for amino acid diversity

  1996cited by this paper
• DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

  1994cited by this paper
• Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide.

  1993cited by this paper
• Improved site-directed mutagenesis method using PCR.

  1991cited by this paper
• Towards a general theory of adaptive walks on rugged landscapes.

  1987cited by this paper

• Engineering Enzymes for Environmental Sustainability

  2023cites this paper
• Engineering Enzymes for Environmental Sustainability

  2023cites this paper
• Engineering of the thermophilic nitrile hydratase from Pseudonocardia thermophila JCM3095 for large-scale nicotinamide production based on sequence-activity relationships.

  2021cites this paper
• Rate-Perturbing Single Amino Acid Mutation for Hydrolases: A Statistical Profiling.

  2021cites this paper
• Rate-enhancing Single Amino Acid Mutation for Hydrolases: A Statistical Profiling

  2021cites this paper
• Extracellular Vesicle–Mediated In Vitro Transcribed mRNA Delivery for Treatment of HER2+ Breast Cancer Xenografts in Mice by Prodrug CB1954 without General Toxicity

  2020cites this paper
• Protein engineering: the potential of remote mutations.

  2019cites this paper
• Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution

  2017cites this paper
• NIH workshop report on the trans-agency blood–brain interface workshop 2016: exploring key challenges and opportunities associated with the blood, brain and their interface

  2017cites this paper
• Determinants and Prediction of Esterase Substrate Promiscuity Patterns.

  2017cites this paper
• Computational Identification of Amino-Acid Mutations that Further Improve the Activity of a Chalcone–Flavonone Isomerase from Glycine max

  2017cites this paper
• Identification of novel members of the bacterial azoreductase family in Pseudomonas aeruginosa.

  2016cites this paper
• Engineering of a fungal nitrilase for improving catalytic activity and reducing by-product formation in the absence of structural information

  2016cites this paper
• Utilizing native fluorescence imaging, modeling and simulation to examine pharmacokinetics and therapeutic regimen of a novel anticancer prodrug

  2016influential citation
• Probabilistic methods in directed evolution: library size, mutation rate, and diversity.

  2014cites this paper
• A web-based tool for rational screening of mutants libraries using ProSAR.

  2014cites this paper
• Improving the glycosylation potential of sucrose phosphorylase through enzyme engineering

  2014cites this paper
• Fitness Loss and Library Size Determination in Saturation Mutagenesis

  2013cites this paper
• Creation and screening of a multi-family bacterial oxidoreductase library to discover novel nitroreductases that efficiently activate the bioreductive prodrugs CB1954 and PR-104A.

  2013cites this paper
• A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium

  2013cites this paper
• Structure Dynamics Guided Enzyme Improvement of ENDO-BETA-1, 4-XYLANASE I

  2013cites this paper
• Improving protein therapeutics through quantitative molecular engineering approaches and a cell-based oral delivery platform

  2013cites this paper
• Computational tools for rational protein engineering of aldolases

  2012cites this paper
• Structure Determination and Functional Analysis of a Chromate Reductase from Gluconacetobacter hansenii

  2012cites this paper
• Gentechnisches Enzymdesign zur Verbesserung der Eigenschaften von Isomaltulose-Synthase aus Protaminobacter rubrum

  2012influential citation
• Rational Design of Enzymes

  2012cites this paper
• Crystal Structure of ChrR—A Quinone Reductase with the Capacity to Reduce Chromate

  2012cites this paper
• Dying for Good: Virus-Bacterium Biofilm Co-evolution Enhances Environmental Fitness

  2012cites this paper
• Advancements of the Sequence Saturation Mutagenesis (SeSaM) Method to Efficiently Explore Protein Sequence Space

  2011cites this paper
• COMPUTATIONAL AND EXPERIMENTAL INVESTIGATION OF THE ENZYMATIC HYDROLYSIS OF CELLULOSE

  2011cites this paper
• Biocatalysts by evolution.

  2010cites this paper
• Enzyme structure dynamics of xylanase I from Trichoderma longibrachiatum

  2010cites this paper
• Discovery and evaluation of Escherichia coli nitroreductases that activate the anti-cancer prodrug CB1954.

  2010cites this paper
• Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

  2010cites this paper
• Improving Biocatalyst Performance by Integrating Statistical Methods into Protein Engineering

  2010influential citation
• Systematic analysis of insertions and deletions specific to nematode proteins and their proposed functional and evolutionary relevance

  2009cites this paper
• Advances in generating functional diversity for directed protein evolution.

  2009cites this paper
• Computational tools for designing and engineering biocatalysts.

  2009cites this paper
• CNOB/ChrR6, a new prodrug enzyme cancer chemotherapy

  2009influential citation