AUTORADIOGRAPHIC LOCALIZATION OF 13N AFTER FIXATION OF 13N-LABELED NITROGEN GAS BY A HETEROCYST-FORMING BLUE-GREEN ALGA

13N, generated by proton bombardment of 13C powder, is rapidly and easily converted to 13N-N2, 0.01 atm pressure, ca. 10 mCi/ml, by automated Dumas combustion. 13N fixed (as 13N-N2) by algal filaments was localized by an autoradiographic technique which permits track autoradiography with isotopes having short half-lives. Our findings show directly that a minimum of about 25% of the N2 fixation by intact, aerobically grown filaments of Anabaena cylindrica is carried out by the heterocysts. If all of the N2 fixation takes place in the heterocysts, then the movement of nitrogen along the filaments can be characterized by a constant τ < ca. 5 s (cell-2).

• Publication year

  1974

• Venue

  Journal of Cell Biology

• Publication date

  1974-05-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1083/JCB.61.2.440PMID 4208073PMCID 2109280
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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