Isolation and partial purification of lysozyme from saliva of Bali cattle ( Bos sondaicus ) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate

Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.

• Publication year

  2012

• Venue

  African Journal of Biotechnology

• Publication date

  2012-01-26

• Fields of study

  Agricultural and Food Sciences, Chemistry, Biology

• Identifiers
  DOI 10.5897/AJB11.2529
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Human Saliva: Clinical Chemistry and Microbiology

  2021cited by this paper
• Salivary Lysozyme and Prevalent Hypertension

  2008cited by this paper
• Properties and application of egg white lysozyme and its modified preparations - a review

  2008influential reference
• Partitioning and purification of lysozyme from chicken egg white using aqueous two-phase system

  2006cited by this paper
• Affinity purification of hen egg lysozyme using sephadex G75

  2006cited by this paper
• Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O‐acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus

  2004cited by this paper
• The Amino Acid Sequence of Satyr Tragopan Lysozyme and Its Activity

  2003cited by this paper
• Antimicrobial Agents in Saliva—Protection for the Whole Body

  2002cited by this paper
• Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: efficacy and safety.

  2002cited by this paper
• A simple bacterial turbidometric method for detection of S. aureus

  2001cited by this paper
• Activity of abundant antimicrobials of the human airway.

  1999cited by this paper
• ImmunoBiology: The Immune System in Health and Diseases

  1996influential reference
• Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus.

  1994cited by this paper
• Factors influencing measurement of human salivary lysozyme in lysoplate and turbidimetric assays

  1986cited by this paper
• Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis

  1985cited by this paper
• Lysozyme in human body fluids.

  1974cited by this paper

• Preventive and Therapeutic Potential of Streptococcus cristatus CA119 in Experimental Periodontitis in Rats

  2024cites this paper
• Bioinformatics analysis of structures and ligand-bindings of predicted zymogen granule protein observed on Bali cattle (Bos javanicus) saliva

  2021cites this paper
• ISOLASI LISOZIM ALBUMIN TELUR AYAM RAS DENGAN METODE KROMATOGRAFI PENUKAR ION

  2018cites this paper
• Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development

  2018cites this paper
• Characterization of some potentially probiotic Lactobacillus strains of human origin

  2015cites this paper
• Proteomic Analysis of a 14.2 kDa Protein Isolated from Bali Cattle (Bos Sondaicus/Javanicus) Saliva Using Single Dimension Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometer

  2013cites this paper
• Proteomic analysis of a 14.2 kDa protein isolated from Bali cattle (Bos sondaicus/javanicus) saliva using 1-D SDS-PAGE gel and MALDITOF-TOF mass spectrometer

  2013cites this paper