Shortage in supply for petroleum-based liquid fuel have promote researches in the area of alternative energy such as blended fuel containing various amount of bio-ethanol derived from waste lignocellulosic biomass. This paper aims to optimize the enzymatic saccharification by varying reaction temperature, enzyme loading and time required to hydrolyzed palm empty fruit bunch (P-EFB). The best saccharification conditions were used to generate reduced sugar, consisting of glucose, fructose which was then passed through the fermentation process to produce bio-ethanol. Optimal saccharification of P-EFB was found to be conducted at temperature of 45°C, enzyme loading of 20 FPU/g and reaction time of 12 hours. The optimum concentration of reduced sugar produced in the pre-hydrolyzed slurry was 11.4 g/L. Fermentation of pre-hydrolyzed slurry in 5 g/L yeast solution was conducted at 40°C and fermenting time of 24 hours. Product from fermentation contained 29.5 g/L bioethanol or bio-ethanol yield of 68.7 %.
Bioethanol production through enzymatic saccharification and fermentation of mechanically milled empty palm bunch
S. Echaroj,N. Pannucharoenwong
Published 2018 in 2018 IEEE 5th International Conference on Engineering Technologies and Applied Sciences (ICETAS)
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- Publication year
2018
- Venue
2018 IEEE 5th International Conference on Engineering Technologies and Applied Sciences (ICETAS)
- Publication date
2018-11-01
- Fields of study
Chemistry, Engineering, Environmental Science
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