Screening of Different Extracts from Artemisia Species for Their Potential Antimalarial Activity

The formation of hemozoin (malaria pigment) has been proposed as an ideal drug target for antimalarial screening programs. In this study, we used an improved, cost-effective and high-throughput spectrophotometric assay to screen plant extracts for finding novel antimalarial plant sources. Fifteen extracts with different polarity from three Iranian Artemisia species, A. ciniformis, A. biennis and A. turanica, were assessed for their antimalarial activity by in-vitro β-hematin formation assay. The most potent effect was observed in dichloromethane (DCM) extract of A. ciniformis with IC50 and IC90 values of 0.92 ± 0.01 and 1.29 ± 0.02 mg/mL, respectively. Ethyl acetate (EtOAC) extracts of A. biennis and A. turanica also showed significant antimalarial activities with IC50 values of 1.11 ± 0.02 and 1.35 ± 0.08 mg/mL and IC90 values of 1.22 ± 0.04 and 2.81 ± 0.21 mg/mL, respectively. Based on these results, it is possible to conclude that the components with strong antimalarial activity have been concentrated in the medium-polar extracts.

• Publication year

  2015

• Venue

  Iranian journal of pharmaceutical research

• Publication date

  2015-05-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.22037/IJPR.2015.1653PMID 25901169PMCID 4403078
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Medium-polar extracts appear to concentrate the components with the strongest antimalarial activity.

  Confidence 0.88

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• Ethyl acetate extracts of Artemisia biennis and Artemisia turanica also showed significant antimalarial activity, with the reported IC50 and IC90 values.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• The dichloromethane extract of Artemisia ciniformis showed the strongest activity, with IC50 and IC90 values of 0.92 ± 0.01 and 1.29 ± 0.02 mg/mL, respectively.

  Confidence 0.99

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• A high-throughput spectrophotometric assay based on β-hematin formation was used to screen Artemisia plant extracts for antimalarial potential.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review

• artemisia biennis

  plant species

  One of the Iranian Artemisia species whose solvent extracts were tested in the screening panel.

  Aliases: A. biennis

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• artemisia ciniformis

  plant species

  One of the Iranian Artemisia species whose solvent extracts were tested in the screening panel.

  Aliases: A. ciniformis

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• artemisia turanica

  plant species

  One of the Iranian Artemisia species whose solvent extracts were tested in the screening panel.

  Aliases: A. turanica

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• dichloromethane extract

  extract

  A plant extract fraction prepared using dichloromethane as the extraction solvent.

  Aliases: DCM extract

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• ethyl acetate extract

  extract

  A plant extract fraction prepared using ethyl acetate as the extraction solvent.

  Aliases: EtOAC extract

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• hemozoin

  biological target

  The malaria pigment whose formation is targeted by the assay as a readout of heme detoxification.

  Aliases: malarial pigment

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• high-throughput spectrophotometric assay

  assay

  A spectrophotometric screening method used to measure inhibition of β-hematin formation in test extracts.

  Aliases: spectrophotometric assay, high-throughput assay

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• medium-polar extracts

  extract fraction

  Extract fractions prepared with solvents of intermediate polarity, including dichloromethane and ethyl acetate fractions.

  Aliases: medium polarity extracts

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review
• β-hematin formation assay

  assay

  An in-vitro assay that evaluates compounds by their ability to inhibit β-hematin crystal formation.

  Aliases: beta-hematin formation assay, hemozoin formation assay

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewq (76h6bfydm6) review

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