In vitro systems for Atg8 lipidation

Highlights • Atg8 lipidation can be efficiently reconstituted in vitro.• Lipidation and de-lipidation of Atg8 by Atg4 can be analyzed.• Reconstitution of Atg8-lipidation using giant unilamellar vesicles offers spatial insights.• These assays allow determining the effect of modifications on Atg8 lipidation/de-lipidation.

• Publication year

  2015

• Venue

  Methods

• Publication date

  2015-03-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.ymeth.2014.11.004PMID 25461810PMCID 4358837
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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