Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Biotechnology progress (Print)

• Publication date

  2011-09-01

• Fields of study

  Biology, Chemistry, Engineering, Environmental Science, Medicine

• Identifiers
  DOI 10.1002/btpr.663PMID 21774095
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2009cited by this paper
• Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel.

  2009cited by this paper
• Expression in the yeast Pichia pastoris.

  2009cited by this paper
• Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbent.

  2008cited by this paper
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• Gene Cloning and Heterologous Expression of a Novel Endoglucanase, Swollenin, from Trichoderma pseudokoningii S38

  2008cited by this paper
• Cellulase kinetics as a function of cellulose pretreatment.

  2008cited by this paper
• Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability.

  2008cited by this paper
• Codon optimization of Bacillus licheniformis β-1,3-1,4-glucanase gene and its expression in Pichia pastoris

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  2006cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
• Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

  2003cited by this paper
• BMC Biotechnology BioMed Central Methodology article Quantitative Real Time Polymerase Chain Reaction for

  2003influential reference
• Optimization of the expression of equistatin in Pichia pastoris.

  2002cited by this paper
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  2002cited by this paper
• Real-Time Quantitative PCR

  2002influential reference
• Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.

  2002cited by this paper
• Effect of single active-site cleft mutation on product specificity in a thermostable bacterial cellulase

  2002cited by this paper
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• Expression of foreign genes in the yeast Pichia pastoris.

  2001cited by this paper
• Recombinant protein expression in Pichia pastoris

  2000cited by this paper
• Heterologous expression and characterization of endoglucanase I (EGI) from Trichoderma viride HK-75.

  1999cited by this paper
• Cellulose degrading enzymes and their potential industrial applications.

  1997cited by this paper
• cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast.

  1997cited by this paper
• Real time quantitative PCR.

  1996cited by this paper
• Structural and functional analysis of Trichoderma reesei endoglucanase I expressed in yeast Saccaromyces cerevisiae

  1991cited by this paper
• Continuous photometric determination of endo-1,4-beta-D-glucanase (cellulase) activity using 4-methylumbelliferyl-beta-D-cellobioside as a substrate.

  1989cited by this paper
• Continuous photometric determination of endo-1,4-β-d-glucanase (cellulase) activity using 4-methylumbelliferyl-β-d-cellobioside as a substrate

  1989cited by this paper
• Cloning, Characterization, and Expression in Saccharomyces Cerevisiae of Endoglucanase I from Trichoderma Reesei

  1987cited by this paper
• Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae

  1987cited by this paper
• A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei.

  1987cited by this paper
• Measurement of cellulase activities

  1987cited by this paper

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  2022cites this paper
• From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi.

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