Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes.

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Cytometry

• Publication date

  2002-04-15

• Fields of study

  Medicine, Mathematics

• Identifiers
  DOI 10.1002/CYTO.10084PMID 12116351
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Quality control of CD4+ T-lymphocyte enumeration: results from the last 9 years of the United Kingdom National External Quality Assessment Scheme for Immune Monitoring (1993-2001).

  2002cited by this paper
• Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. European Working Group on Clinical Cell Analysis.

  2000cited by this paper
• Prophylaxis against opportunistic infections in patients with human immunodeficiency virus infection.

  2000cited by this paper
• Evaluation of TruCount Absolute-Count Tubes for Determining CD4 and CD8 Cell Numbers in Human Immunodeficiency Virus-Positive Adults

  2000cited by this paper
• Reduction of intra‐ and interlaboratory variation in CD34+ stem cell enumeration using stable test material, standard protocols and targeted training

  2000cited by this paper
• Multisite Comparison of CD4 and CD8 T-Lymphocyte Counting by Single- versus Multiple-Platform Methodologies: Evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System

  2000cited by this paper
• Comparison of immune reconstitution after unrelated and related T-cell-depleted bone marrow transplantation: effect of patient age and donor leukocyte infusions.

  1999cited by this paper
• Absolute CD4+ T‐lymphocyte and CD34+ stem cell counts by single‐platform flow cytometry: the way forward

  1999cited by this paper
• CD4 lymphocytopenia as a risk factor for skin cancers in renal transplant recipients.

  1998cited by this paper
• Evaluation of a flow cytometric method for simultaneous leukocyte phenotyping and quantification by fluorescent microspheres.

  1998cited by this paper
• Impact of unified procedures as implemented in the Canadian Quality Assurance Program for T lymphocyte subset enumeration. Participating Flow Cytometry Laboratories of the Canadian Clinical Trials Network for HIV/AIDS Therapies.

  1998cited by this paper
• Analysis of variation in results of flow cytometric lymphocyte immunophenotyping in a multicenter study.

  1997cited by this paper
• Evaluation of a method for counting absolute numbers of cells with a flow cytometer

  1997cited by this paper
• Guidelines for the enumeration of CD4+ T lymphocytes in immunosuppressed individuals. CD4+ T lymphocyte Working Party. Members of the General Haematology Task Force of BCSH.

  1997cited by this paper
• Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping.

  1996cited by this paper
• Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry.

  1996cited by this paper
• Evaluation of a novel stable whole blood quality control material for lymphocyte subset analysis: results from the UK NEQAS immune monitoring scheme.

  1996cited by this paper
• Standardization of absolute CD4+ lymphocyte counts across laboratories: an evaluation of the Ortho CytoronAbsolute flow cytometry system on normal donors.

  1995cited by this paper
• Guideline for flow cytometric immunophenotyping: a report from the National Institute of Allergy and Infectious Diseases, Division of AIDS.

  1993cited by this paper
• Improved Precision in the Enumeration of Absolute Numbers of Lymphocyte Phenotypes with Long‐Term Monthly Proficiency Testing

  1993cited by this paper
• CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay.

  1993cited by this paper
• A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol. Comparison with conventional two-color immunophenotyping method.

  1992cited by this paper
• Recovery from cytomegalovirus infection is associated with activation of peripheral blood lymphocytes.

  1992cited by this paper
• Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry.

  1990cited by this paper
• The effect of cytomegalovirus infection on T lymphocytes after allogeneic bone marrow transplantation.

  1985cited by this paper
• Increased numbers of cells with suppressor T cell phenotype in the peripheral blood of patients with infectious mononucleosis.

  1981cited by this paper
• Analysis of T lymphocyte subsets in cytomegalovirus mononucleosis.

  1981cited by this paper

• Deep Immunophenotyping of Human Whole Blood by Standardized Multi-parametric Flow Cytometry Analyses

  2023cites this paper
• Risk factors for T‐cell lymphopenia in frequent platelet donors: The BEST collaborative study

  2023cites this paper
• Differential blood leukocyte populations based on individual variances and age

  2022cites this paper
• Cytoflow: A Python Toolbox for Flow Cytometry

  2022cites this paper
• Automated lightless cytometry on a microchip with adaptive immunomagnetic manipulation.

  2022cites this paper
• Optimized Protocols for Isolation, Fixation and Flow Cytometric Characterization of Leukocytes in Ischemic Hearts.

  2019cites this paper
• The quest for equivalence of test results: the pilgrimage of the Dutch Calibration 2.000 program for metrological traceability

  2018cites this paper
• Early Macrophage Activation in Preterm Newborns and Respiratory Disease

  2017cites this paper
• Optimization of individualized graft composition: CD3/CD19 depletion combined with CD34 selection for haploidentical transplantation

  2016cites this paper
• Cell-based monitoring of cancer : Circulating tumor and endothelial cells

  2015cites this paper
• AUTOMATED GATING OF PORTABLE CYTOMETER DATA BASED ON SKEW t MIXTURE MODELS

  2015cites this paper
• Lymphocyte subsets: Reference ranges in an age‐ and gender‐balanced population of Omani healthy adults

  2013cites this paper
• Monoclonal B lymphocytosis versus chronic lymphocytic leukemia: Factors affecting implementation of an absolute threshold

  2013cites this paper
• Computational exploratory analysis of high-dimensional Flow Cytometry data for diagnosis and biomarker discovery

  2013cites this paper
• Single-color Multitarget Flow Cytometry Using Monoclonal Antibodies Labeled with Different Intensities of the Same Fluorochrome

  2012cites this paper
• Validation and Quality Control in Clinical Flow Cytometry

  2012cites this paper
• Computational techniques for flow cytometry : the application for automated analysis of innate immune response flow cytometry data.

  2012cites this paper
• External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study

  2011cites this paper
• Rapid cell population identification in flow cytometry data

  2011cites this paper
• External Quality Assurance of Circulating Tumor Cell Enumeration Using

  2011cites this paper
• Establishment of reference CD4+ T cell values for adult Indian population

  2011cites this paper
• PIMA™ CD4 AS COMPARED TO BD FACSCALIBER FLOW CYTOMETRY

  2010cites this paper
• Potential of a cytomics top-down strategy for drug discovery.

  2010cites this paper
• The impact of a CD4 external quality assessment programme for Southern Africa and Africa

  2010cites this paper
• A review on peripheral blood CD4+ T lymphocyte counts in healthy adult Indians

  2010cites this paper
• CONTROLLING LABORATORY VARIABLES TO IMPROVE PRECISION AND ACCURACY OF CD4+ T-CELL ENUMERATION ACROSS FLOW CYTOMETRY METHODS.

  2009influential citation
• Comparison of interlaboratory variation in absolute T‐cell counts by single‐platform and optimized dual‐platform methods

  2009cites this paper
• Merging Mixture Components for Cell Population Identification in Flow Cytometry

  2009cites this paper
• Evaluation of a multicolor , single tube technique for the determination of lymphocyte subpopulations

  2008cites this paper
• Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries

  2008influential citation
• ISHAGE-based single-platform flowcytometric analysis for measurement of absolute viable T cells in fresh or cryopreserved products: CD34/CD133 selected or CD3/CD19 depleted stem cells, DLI and purified CD56+CD3− NK cells

  2008cites this paper
• Evaluating new CD4 enumeration technologies for resource-constrained countries

  2008cites this paper
• African regional external quality assessment for CD4 T‐cell enumeration: Development, outcomes, and performance of laboratories

  2008cites this paper
• Evaluation of a Multicolor, Single-Tube Technique To Enumerate Lymphocyte Subpopulations

  2008cites this paper
• Long‐term stabilized blood samples as controls for flow cytometric HLA‐B27 screening: A feasibility study

  2008cites this paper
• Challenges in implementing CD4 testing in resource‐limited settings

  2008cites this paper
• Analytical performance of a standardized single‐platform MHC tetramer assay for the identification and enumeration of CMV‐specific CD8+ T lymphocytes

  2008cites this paper
• Large‐scale affordable Panleucogated CD4+ testing with proactive internal and external quality assessment: In support of the South African national comprehensive care, treatment and management programme for HIV and AIDS

  2008cites this paper
• Development and validation of stabilized whole blood samples expressing T-cell activation markers as quality control reference material

  2008cites this paper
• Twenty years of external quality assurance in clinical cell analysis — A tribute to Jean‐Luc D'Hautcourt

  2007cites this paper
• Severe interference between retinal angiography and automated four‐color flow cytometry analysis of blood mononuclear cells

  2007cites this paper
• Influence of 4‐ and 6‐color flow cytometers and acquisition/analysis softwares on the determination of lymphocyte subsets in HIV infection

  2007cites this paper
• External quality assessment in flow cytometry: educational aspects and trends toward improvement

  2007cites this paper
• Variations in Plasmacytoid Dendritic Cell (PDC) and Myeloid Dendritic Cell (MDC) Levels in HIV-Infected Subjects on and off Antiretroviral Therapy

  2006cites this paper
• Flow cytometric assessment of autologous γδ T cells in patients with acute myeloid leukemia: Potential effector cells for immunotherapy?

  2006cites this paper
• Flow cytometric assessment of autologous gammadelta T cells in patients with acute myeloid leukemia: potential effector cells for immunotherapy?

  2006cites this paper
• Kalibratie 2000: de stand van zaken, de relatie met de IVD-richtlijn, de toekomst

  2005cites this paper
• Multicentre evaluation of stable reference whole blood for enumeration of lymphocyte subsets by flow cytometry.

  2005cites this paper
• Lymphocyte subsets' reference ranges in an age- and gender-balanced population of 100 healthy adults--a monocentric German study.

  2005cites this paper
• Quantitation of MHC tetramer‐positive cells from whole blood: Evaluation of a single‐platform, six‐parameter flow cytometric method

  2004cites this paper
• Enumeration of antigen‐specific CD8+ T lymphocytes by single‐platform, HLA tetramer‐based flow cytometry: A European multicenter evaluation

  2004influential citation
• Establishment of Adult Peripheral Blood Lymphocyte Subset Reference Range for an Asian Population by Single-Platform Flow Cytometry: Influence of Age, Sex, and Race and Comparison with Other Published Studies

  2004cites this paper
• Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland

  2004cites this paper
• Impact of standardization on clinical cell analysis by flow cytometry.

  2004cites this paper
• The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes.

  2004cites this paper
• A REVIEW OF CD4 MONITORING BY FLOW CYTOMETRY

  2003cites this paper
• European Working Group on Clinical Cell Analysis (EWGCCA): 6 years on.

  2002cites this paper
• Quality control of CD4+ T-lymphocyte enumeration: results from the last 9 years of the United Kingdom National External Quality Assessment Scheme for Immune Monitoring (1993-2001).

  2002cites this paper
• Morbidity and Mortality Weekly Report Guidelines for Performing Single-platform Absolute Cd4 + T-cell Determinations with Cd45 Gating for Persons Infected with Human Immunodeficiency Virus Guidelines for Using the Quantiferon ® -tb Test for Diagnosing Latent Mycobacterium Tuberculosis Infection Mmwr

  year unknowncites this paper
• Guidelines for Performing Single-Platform Absolute CD4 + T-Cell Determinations with CD45 Gating for Persons Infected with Human Immunodeficiency Virus Guidelines for Using the QuantiFERON ® -TB Test for Diagnosing Latent Mycobacterium tuberculosis Infection

  year unknowncites this paper