The M1 and M2 subunits of rat pyruvate kinase are encoded by different messenger RNAs.

The question of whether the M1- and M2-type isozymes of pyruvate kinase are synthesized by a common mRNA was examined by isolating total RNA from rat skeletal muscle and AH-130 Yoshida ascites hepatoma cells, which express the M1- and M2-type isozymes, respectively, and translating the preparations in a rabbit reticulocyte lysate system. The newly synthesized pyruvate kinase subunits were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing under the denaturing condition. The pyruvate kinase subunit synthesized from hepatoma cell RNA had a slightly larger molecular weight and higher pI value than the subunit from muscle RNA. When a mixture of the two RNAs was added to the lysate, the same result was obtained. Thus, it is concluded that the M1- and M2-type isozymes are translated from different messenger RNAs.

• Publication year

  1982

• Venue

  Journal of Biological Chemistry

• Publication date

  1982-02-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(19)68155-xPMID 7056711
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Purification and properties of mouse pyruvate kinases K and M and of a modified K subunit.

  1981cited by this paper
• Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.

  1980cited by this paper
• Regulation of hepatoma tissue culture cell tyrosine aminotransferase messenger ribonucleic acid by dexamethasone.

  1980cited by this paper
• Peptide mapping by limited proteolysis of four pyruvate kinase isozymes

  1978cited by this paper
• Use of staphylococcal protein A as an immunological reagent.

  1978cited by this paper
• Purification of four pyruvate kinase isozymes of rats by affinity elution chromatography.

  1978cited by this paper
• Dibutyryl cyclic AMP increases the amount of functional messenger RNA coding for tyrosine aminotransferase in rat liver.

  1978cited by this paper
• Pyruvate kinase isozymes in man

  1976cited by this paper
• An efficient mRNA-dependent translation system from reticulocyte lysates.

  1976cited by this paper
• Pyruvate kinase isozymes in various tissues of rat, and increase of spleen-type pyruvate kinase in liver by injecting chromatins from spleen and tumor.

  1976cited by this paper
• Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

  1975cited by this paper
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper
• Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

  1974cited by this paper
• Studies on pyruvate kinase (PK) deficiency. II. Electrophoretic, kinetic and immunological studies on pyruvate kinase of erythrocytes and other tissues.

  1973cited by this paper
• Multimolecular forms of pyruvate kinase from rat and other mammalian tissues. I. Electrophoretic studies.

  1972cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Crystallization, characterization and metabolic regulation of two types of pyruvate kinase isolated from rat tissues.

  1967cited by this paper

• Are allosteric mechanisms conserved among homologues?

  2025cites this paper
• A promising frontier: targeting NETs for stroke treatment breakthroughs

  2024cites this paper
• PKM2 promotes neutrophil activation and cerebral thromboinflammation: therapeutic implications for ischemic stroke

  2021cites this paper
• Nuclear Factor 1 Family Members Interact with Hepatocyte Nuclear Factor 1α to Synergistically Activate L-type Pyruvate Kinase Gene Transcription*

  2005cites this paper
• Regulation of rat liver L-type pyruvate kinase mRNA by insulin and by fructose.

  2005cites this paper
• Discrimination between neuronal and glial cell tumours by pyruvate kinase electrophoresis

  2005cites this paper
• Cysteamine prevents and reverses the inhibition of pyruvate kinase activity caused by cystine in rat heart.

  2004cites this paper
• The oval-shaped cell as a candidate for a liver stem cell in embryonic, neonatal and precancerous liver: identification based on morphology and immunohistochemical staining for albumin and pyruvate kinase isoenzyme expression

  1997cites this paper
• Structure and function of manganese-containing biomolecules

  1996cites this paper
• The molecular basis of canine pyruvate kinase deficiency.

  1994cites this paper
• Pyruvate Kinase in Selected Human Tumors

  1991cites this paper
• Alteration in L-type pyruvate kinase gene expression is not associated with the LF-B1 mRNA level.

  1991cites this paper
• Characterization of pyruvate kinase from human rhabdomyosarcoma in relation to immunohistochemical and morphological criteria

  1989cites this paper
• The liver/erythrocyte pyruvate kinase gene complex [Pk-1] in the mouse: structural gene mutations.

  1989cites this paper
• Phylogeny congruence analysis and isozyme classification: the pyruvate kinase system.

  1989cites this paper
• Rat pyruvate kinase M gene. Its complete structure and characterization of the 5'-flanking region.

  1989cites this paper
• Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide

  1988cites this paper
• Pyruvate kinase isoenzyme transitions in cultures of fetal rat hepatocytes.

  1988cites this paper
• [Structures of pyruvate kinase isozyme genes and regulation of their expressions].

  1988cites this paper
• The L- and R-type isozymes of rat pyruvate kinase are produced from a single gene by use of different promoters.

  1987cites this paper
• Evolution in glycolysis.

  1987cites this paper
• Effects of aging on contributions of dietary fat and triiodothyronine treatment to lipogenic enzyme induction.

  1987cites this paper
• Effects of aging on contributions of dietary fat and triiodothyronine treatment to lipogenic enzyme induction.

  1987cites this paper
• Purification of pyruvate kinase isoenzymes type M1 and M2 from dog (Canis familiaris) and comparison of their properties with those from chicken and rat.

  1986cites this paper
• Effects of dietary nutrients on lipogenic enzyme and mRNA activities in rat liver during induction.

  1986cites this paper
• Carbohydrate metabolism in neoplastic tissue.

  1986cites this paper
• Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence.

  1986cites this paper
• The structure of cat muscle pyruvate kinase.

  1986cites this paper
• Pyruvate kinase and total protein are regulated differently during growth of P-815 mastocytoma cells.

  1986cites this paper
• The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing.

  1986cites this paper
• Effects of dietary proteins on lipogenic enzymes in rat liver.

  1986cites this paper
• Transcriptional and post-transcriptional regulation of L-type pyruvate kinase in diabetic rat liver by insulin and dietary fructose.

  1985cites this paper
• Molecular aspects of erythroenzymopathies associated with hereditary hemolytic anemia

  1985cites this paper
• Regulation of Pyruvate Kinase Gene Expression by Hormones and Developmental Factors

  1985cites this paper
• Pancreatic islets contain the M2 isoenzyme of pyruvate kinase its phosphorylation has no effect on enzyme activity

  1985cites this paper
• The purification and kinetic characterization of eel white muscle pyruvate kinase.

  1985cites this paper
• Rapid regulation of L-type pyruvate kinase mRNA by fructose in diabetic rat liver.

  1984cites this paper
• Purification, catalytic and regulatory properties of Rana ridibunda erythrocyte pyruvate kinase.

  1984cites this paper
• Molecular cloning of cDNA sequences for rat M2-type pyruvate kinase and regulation of its mRNA.

  1984cites this paper
• The Pk-3 gene determines both the heart, M1, and the kidney, M2, pyruvate kinase isozymes in the mouse; and a simple electrophoretic method for separating phosphoglucomutase-3

  1984cites this paper
• Muscle and liver pyruvate kinases are closely related: amino acid sequence comparisons

  1984cites this paper
• Molecular cloning of DNA complementary to rat L-type pyruvate kinase mRNA. Nutritional and hormonal regulation of L-type pyruvate kinase mRNA concentration.

  1983cites this paper
• Induction of rat liver malic enzyme messenger RNA activity by insulin and by fructose.

  1983cites this paper
• Human Tumors: Enzyme Activities And Isozyme Profiles

  1983cites this paper
• Structural and kinetic differences between the M2 type pyruvate kinases from lung and various tumors.

  1983cites this paper
• The M1 and M2 isozymes of pyruvate kinase are the products of the same gene.

  1982cites this paper
• Two different species of messenger RNAs specify synthesis of M1 and M2 pyruvate kinase subunits.

  1982cites this paper
• Peptide structures of pyruvate kinase isozymes. I. Comparison of the four pyruvate kinase isozymes of the rat.

  1982cites this paper
• Peptide structures of pyruvate kinase isozymes: 2. Origins of types M1 and M2 isozymes suggested from species-variations in their peptide maps.

  1982cites this paper
• Effect of aging on induction of rat liver messenger RNA activity for malic enzyme.

  1982cites this paper
• Pyruvate kinase isozymes from rat.

  1982cites this paper
• [25] Pyruvate kinase isozymes from rat

  1982cites this paper