Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in Arabidopsis thaliana

ABSTRACT The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of Arabidopsis thaliana. Biochemical fractionation of A. thaliana cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic A. thaliana exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the ago4 phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing. Summary: CMV suppressor 2b perturbs host defense by interrupting epigenetic signaling. 2b causes FWA siRNAs sequestration and hypo-methylation of FWA promoter region. It shines light on a hitherto little understood mechanism of viral suppression of host epigenetics.

• Publication year

  2016

• Venue

  Biology Open

• Publication date

  2016-09-22

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1242/bio.017244PMID 27659688PMCID 5155521
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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