Tracking individual secretory vesicles during exocytosis reveals an ordered and regulated process

An analysis of individual secretory vesicles during yeast exocytosis explores how Sec4-GTP hydrolysis, Myo2p motors, an SM protein, and exocyst components contribute to the timing of vesicle tethering and fusion.

• Publication year

  2015

• Venue

  Journal of Cell Biology

• Publication date

  2015-07-20

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201501118PMID 26169352PMCID 4508886
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Head-to-tail regulation is critical for the in vivo function of myosin V

  2015cited by this paper
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  2014cited by this paper
• Phosphorylation of the Rab exchange factor Sec2p directs a switch in regulatory binding partners

  2013influential reference
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  2013cited by this paper
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  2012influential reference
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  2012cited by this paper
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  2011cited by this paper
• PI4P and Rab inputs collaborate in myosin-V-dependent transport of secretory compartments in yeast.

  2011influential reference
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  2011cited by this paper
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  2007cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2005cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2002cited by this paper
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  1999cited by this paper
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  1995cited by this paper
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  1994cited by this paper
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  1992cited by this paper
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  1991cited by this paper
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  1991cited by this paper
• Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton.

  1989cited by this paper
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  1987cited by this paper
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  1983cited by this paper
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  1980cited by this paper

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