DNA Fingerprinting of Mycobacterium tuberculosis: Lessons Learned and Implications for the Future

DNA fingerprinting of Mycobacterium tuberculosis—a relatively new laboratory technique—offers promise as a powerful aid in the prevention and control of tuberculosis (TB). Established in 1996 by the Centers for Disease Control and Prevention (CDC), the National Tuberculosis Genotyping Surveillance Network was a 5-year prospective, population-based study of DNA fingerprinting conducted from 1996 to 2000. The data from this study suggest multiple molecular epidemiologic and program management uses for DNA fingerprinting in TB public health practice. From these data, we also gain a clearer understanding of the overall diversity of M. tuberculosis strains as well as the presence of endemic strains in the United States. We summarize the key findings and the impact that DNA fingerprinting may have on future approaches to TB control. Although challenges and limitations to the use of DNA fingerprinting exist, the widespread implementation of the technique into routine TB prevention and control practices appears scientifically justified.

• Publication year

  2002

• Venue

  Emerging Infectious Diseases

• Publication date

  2002-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3201/eid0811.020402PMID 12453363PMCID 2738558
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Tuberculosis Skin Testing Among Homeless Adults

  2006cited by this paper
• A Prospective, Multicenter Study of Laboratory Cross-Contamination of Mycobacterium tuberculosis Cultures

  2002cited by this paper
• Genotyping Analyses of Tuberculosis Cases in U.S.- and Foreign-Born Massachusetts Residents

  2002cited by this paper
• Genotype analysis of Mycobacterium tuberculosis isolates from a sentinel surveillance population.

  2002cited by this paper
• Transmission of Mycobacterium tuberculosis in a Rural Community, Arkansas, 1945–2000

  2002cited by this paper
• Molecular epidemiology of tuberculosis in a low- to moderate-incidence state: are contact investigations enough?

  2002cited by this paper
• Identifying the Sources of Tuberculosis in Young Children: A Multistate Investigation

  2002cited by this paper
• Molecular Epidemiology of Tuberculosis in a Sentinel Surveillance Population

  2002cited by this paper
• National Tuberculosis Genotyping and Surveillance Network: Analysis of the Genotype Database

  2002cited by this paper
• Estimated Costs of False Laboratory Diagnoses of Tuberculosis in Three Patients

  2002cited by this paper
• National Tuberculosis Genotyping and Surveillance Network: Design and Methods

  2002cited by this paper
• DNA Fingerprinting of Mycobacterium tuberculosis Isolates from Epidemiologically Linked Case Pairs

  2002cited by this paper
• Human Exposure following Mycobacterium tuberculosis Infection of Multiple Animal Species in a Metropolitan Zoo

  2002cited by this paper
• Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network

  2002cited by this paper
• Use of DNA Fingerprinting To Investigate a Multiyear, Multistate Tuberculosis Outbreak

  2002cited by this paper
• Spoligologos: A Bioinformatic Approach to Displaying and Analyzing Mycobacterium tuberculosis Data

  2002cited by this paper
• Changes in the transmission of tuberculosis in New York.

  2002cited by this paper
• Molecular Differentiation of Mycobacterium tuberculosis Strains without IS6110 Insertions

  2002cited by this paper
• Cross-Jurisdictional Transmission of Mycobacterium tuberculosis in Maryland and Washington, D.C., 1996–2000, Linked to the Homeless

  2002cited by this paper
• Prospective use of molecular typing of Mycobacterium tuberculosis by use of restriction fragment-length polymorphism in a public tuberculosis-control program.

  2002cited by this paper
• Methodological problems in the molecular epidemiology of tuberculosis.

  2002cited by this paper
• Skin-Test Screening and Tuberculosis Transmission among the Homeless

  2002cited by this paper
• Mycobacterium tuberculosis Transmission between Cluster Members with Similar Fingerprint Patterns

  2002cited by this paper
• Changes in the transmission of tuberculosis in New York City from 1990 to 1999.

  2002cited by this paper
• Impact of Genotyping of Mycobacterium tuberculosis on Public Health Practice in Massachusetts

  2002cited by this paper
• Molecular Typing of Mycobacterium tuberculosis Strains with a Common Two-Band IS6110 Pattern

  2002cited by this paper
• Molecular Epidemiology of Multidrug-Resistant Tuberculosis, New York City, 1995–1997

  2002cited by this paper
• Mathematical Epidemiology of Infectious Diseases. Eds. O. Dickmann and J. A. P. Heesterbeck. John Wiley & Sons Ltd, 2000. Pp. 250 (hb) £80; (pb) £39.95.

  2001cited by this paper
• The effect of age and study duration on the relationship between ‘clustering’ of DNA fingerprint patterns and the proportion of tuberculosis disease attributable to recent transmission

  2001cited by this paper
• Targeted tuberculin testing and treatment of latent tuberculosis infection. American Thoracic Society.

  2000cited by this paper
• Trends in Tuberculosis Transmission.

  2000cited by this paper
• Spread of strain W, a highly drug-resistant strain of Mycobacterium tuberculosis, across the United States.

  1999cited by this paper
• Influence of sampling on estimates of clustering and recent transmission of Mycobacterium tuberculosis derived from DNA fingerprinting techniques.

  1999cited by this paper
• Dissemination of Mycobacterium tuberculosis across the San Francisco Bay Area.

  1998cited by this paper
• Investigation of cross contamination in a Mycobacterium tuberculosis laboratory using IS6110 DNA fingerprinting.

  1998cited by this paper
• Cross-contamination of specimens with Mycobacterium tuberculosis: clinical significance, causes, and prevention.

  1998cited by this paper
• Mycobacterium tuberculosis Specimen Contamination Revisited: The Role of Laboratory Environmental Control in a Pseudo-outbreak

  1998cited by this paper
• An outbreak involving extensive transmission of a virulent strain of Mycobacterium tuberculosis.

  1998cited by this paper
• Interpretation of restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates from a state with a large rural population.

  1997cited by this paper
• DNA fingerprinting with two probes decreases clustering of Mycobacterium tuberculosis.

  1997cited by this paper
• Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprint analysis.

  1997cited by this paper
• Detection of laboratory cross-contamination of Mycobacterium Mycobacteriu cultures.

  1997cited by this paper
• Detection of Laboratory Cross-Contamination of Mycobacterium tuberculosis Cultures

  1997cited by this paper
• The incidence of false-positive cultures for Mycobacterium tuberculosis.

  1997cited by this paper
• Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis

  1996cited by this paper
• Tuberculosis prophylaxis in the homeless. A trial to improve adherence to referral.

  1996cited by this paper
• The transcontinental transmission of tuberculosis: A molecular epidemiological assessment.

  1996cited by this paper
• Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family.

  1996cited by this paper
• The molecular epidemiology of tuberculosis in New York City: the importance of nosocomial transmission and laboratory error.

  1996cited by this paper
• Transmission of tuberculosis among the urban homeless.

  1996cited by this paper
• Tuberculosis in the homeless.

  1995cited by this paper
• Tuberculosis screening among homeless persons with AIDS living in single-room-occupancy hotels.

  1995cited by this paper
• Endemic tuberculosis among homeless men in New York City.

  1994cited by this paper
• Restriction fragment length polymorphism analysis detecting a community-based tuberculosis outbreak among persons infected with human immunodeficiency virus.

  1994cited by this paper
• HIV and tuberculosis infection in San Francisco's homeless adults. Prevalence and risk factors in a representative sample.

  1994cited by this paper
• Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods.

  1994cited by this paper
• The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods.

  1994cited by this paper
• Nosocomial outbreak of tuberculosis in a renal transplant unit: application of a new technique for restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates.

  1993cited by this paper
• An outbreak of tuberculosis with accelerated progression among persons infected with the human immunodeficiency virus. An analysis using restriction-fragment-length polymorphisms.

  1992cited by this paper
• An outbreak of multidrug-resistant tuberculosis among hospitalized patients with the acquired immunodeficiency syndrome.

  1992cited by this paper
• Outbreak of multidrug-resistant tuberculosis--Texas, California, and Pennsylvania.

  1990cited by this paper
• False-positive cultures of Mycobacterium tuberculosis.

  1984cited by this paper

• Use of network analysis multidrug‐resistant tuberculosis contact investigation in Kanchanaburi, Thailand

  2019cites this paper
• Modelling heterogeneity in host susceptibility to tuberculosis and its effect on public health interventions

  2018cites this paper
• A brief primer on genomic epidemiology: lessons learned from Mycobacterium tuberculosis

  2017cites this paper
• DNA Fingerprinting of Mycobacterium TB : A Rich Source of Fundamental and Daily Applicable Knowledge

  2017cites this paper
• Backward bifurcation and reinfection in mathematical models of tuberculosis

  2017cites this paper
• Practical aspects of backward bifurcation in a mathematical model for tuberculosis.

  2016cites this paper
• Mycobacteria

  2016cites this paper
• A field-validated approach using surveillance and genotyping data to estimate tuberculosis attributable to recent transmission in the United States.

  2015cites this paper
• How host heterogeneity governs tuberculosis reinfection?

  2012cites this paper
• Tuberculosis cross-species transmission in Tanzania: towards a One-Health concept.

  2012cites this paper
• Identification of factors for tuberculosis transmission via an integrated multidisciplinary approach.

  2011cites this paper
• Molecular epidemiology of mycobacterium tuberculosis in the United Arab Emirates

  2011cites this paper
• Three‐year longitudinal study of genotypes of Mycobacterium tuberculosis in a low prevalence population

  2010cites this paper
• Modeling tuberculosis:a compromise between biological realism and mathematical tractability

  2009cites this paper
• MOLECULAR EPIDEMIOLOGY OF TUBERCULOSIS IN PRAGUE AND SOUTH MORAVIA , CZECH REPUBLIC : GENETIC ANALYSIS OF MYCOBACTERIUM TUBERCULOSIS ISOLATES BY IS 6110-RFLP FINGERPRINTING AND

  2008cites this paper
• Genotyping and its implications for transmission dynamics and tuberculosis

  2008cites this paper
• Transmission Classification Model To Determine Place and Time of Infection of Tuberculosis Cases in an Urban Area

  2008cites this paper
• Factors Associated with Differences between Conventional Contact Tracing and Molecular Epidemiology in Study of Tuberculosis Transmission and Analysis in the City of Barcelona, Spain

  2008cites this paper
• Public Health Chronicles

  2007cites this paper
• Risk of acquired drug resistance during short-course directly observed treatment of tuberculosis in an area with high levels of drug resistance.

  2007cites this paper
• Tuberculosis transmission in a high incidence area: a retrospective molecular epidemiological study of Mycobacterium tuberculosis in Casablanca, Morocco.

  2007cites this paper
• Prediction of the tuberculosis reinfection proportion from the local incidence.

  2007cites this paper
• American Thoracic Society/Centers for Disease Control and Prevention/Infectious Diseases Society of America: Controlling Tuberculosis in the United States

  2007cites this paper
• Tuberculosis Control: Challenges of an Ancient and Ongoing Epidemic

  2007cites this paper
• Disseminated tuberculosis: a 10-year experience in a medical center.

  2007cites this paper
• Why rich countries should care about the world's least healthy people.

  2007cites this paper
• Métodos aplicados à epidemiologia molecular do Mycobacterium tuberculosis

  2007cites this paper
• Morbidity and Mortality Weekly Report

  2006cites this paper
• Which patients' factors predict the rate of growth of Mycobacterium tuberculosis clusters in an urban community?

  2006cites this paper
• Molecular Epidemiology of Mycobacterium tuberculosis

  2005cites this paper
• The molecular epidemiology of tuberculosis. Recent trends in a low burden country.

  2005cites this paper
• Molecular epidemiology: a tool for understanding control of tuberculosis transmission.

  2005cites this paper
• Effects of genetic variability of Mycobacterium tuberculosis strains on the presentation of disease.

  2005cites this paper
• Unsuspected recent transmission of tuberculosis among high-risk groups: implications of universal tuberculosis genotyping in its detection.

  2005cites this paper
• IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolates from an area of Casablanca, Morocco.

  2005cites this paper
• [Molecular epidemiology of tuberculosis: main findings and their application in Spain].

  2005cites this paper
• Epidemiología molecular de la tuberculosis: principales hallazgos y su aplicación en España

  2005cites this paper
• American Thoracic Society/Centers for Disease Control and Prevention/Infectious Diseases Society of America: controlling tuberculosis in the United States.

  2005cites this paper
• TB and not TB: Mycobacteriology in Australia in the 21st century

  2004cites this paper
• Investigation of healthcare‐associated transmission of Mycobacterium tuberculosis among patients with malignancies at three hospitals and at a residential facility

  2004cites this paper
• The Arden House Conference on Tuberculosis, revisited: perspectives for tuberculosis elimination in the United States.

  2004cites this paper
• Application of Mycobacterial Interspersed Repetitive Unit Typing to Manitoba Tuberculosis Cases: Can Restriction Fragment Length Polymorphism Be Forgotten?

  2004cites this paper
• Stable association between strains of Mycobacterium tuberculosis and their human host populations.

  2004cites this paper
• Added epidemiologic value to tuberculosis prevention and control of the investigation of clustered genotypes of Mycobacterium tuberculosis isolates.

  2004cites this paper
• Prospective Molecular Epidemiological Study on Transmission of Pulmonary Tuberculosis and Migration (MuT Study)

  2004cites this paper
• Molecular epidemiology of tuberculosis in Prague and South Moravia, Czech Republic: genetic analysis of Mycobacterium tuberculosis isolates by IS6110-RFLP fingerprinting and spoligotyping.

  2004cites this paper
• Morbidity and Mortality Weekly Report

  2004cites this paper
• Evaluation of conventional and molecular strategies for the rapid diagnosis and molecular characterisation of strains of Mycobacterium tuberculosis

  2003cites this paper
• Mycobacterium tuberculosis Beijing Genotype1

  2003cites this paper
• Molecular epidemiology of tuberculosis.

  2003cites this paper
• Iranian Journal of Basic Medical Sciences Comparison of the Genetic Convergence between Myco- Bacterium Strains by Three Rflp-based Methods in Central Province of Iran

  year unknowncites this paper