Dihydrofolate reductase as a model for studies of enzyme dynamics and catalysis

Dihydrofolate reductase from Escherichia coli (ecDHFR) serves as a model system for investigating the role of protein dynamics in enzyme catalysis. We discuss calculations predicting a network of dynamic motions that is coupled to the chemical step catalyzed by this enzyme. Kinetic studies testing these predictions are presented, and their potential use in better understanding the role of these dynamics in enzyme catalysis is considered. The cumulative results implicate motions across the entire protein in catalysis.

• Publication year

  2015

• Venue

  F1000Research

• Publication date

  2015-12-17

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.12688/f1000research.6968.1PMID 26918149PMCID 4754028
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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