Purification and Properties of Periplasmic 3′:5′-Cyclic Nucleotide Phosphodiesterase

The 3′:5′-cyclic nucleotide phosphodiesterase (CNP) of Vibrio fischeri, due to its unusual location in the periplasm, allows this symbiotic bacterium to utilize extracellular 3′:5′-cyclic nucleotides (e.g. cAMP) as sole sources of carbon and energy, nitrogen, and phosphorus for growth. The enzyme was purified to apparent homogeneity by a four-step procedure: chloroform shock, ammonium sulfate precipitation, and chromotography on DEAE-Sephacel and Cibacron Blue 3GA-agarose. The active enzyme consists of a single polypeptide with a mass of 34 kDa. At 25°C, it has a pH optimum of 8.25, a K for cAMP of 73 μm, and a Vmax of 3700 μmol of cAMP hydrolyzed/min/mg protein (turnover number of 1.24 105/min). The specific activity of the V. fischeri enzyme is approximately 20-fold greater than that of any previously characterized CNP when comparisons of activity are made at the same assay temperature. Activity increases with temperature up to 60°C. The CNP contains 2 atoms of zinc/monomer, and zinc, copper, magnesium, and calcium can restore activity of the apoenzyme to varying degrees. The exceptional specific activity of the enzyme and its unusual location in the periplasm support proposals that the enzyme enables the bacterium to scavenge 3′:5′-cyclic nucleotides in seawater and that the enzyme plays a role in cAMP-mediated host-symbiont interactions.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-07-21

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.270.29.17627PMID 7615571
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The light organ symbiont Vibrio fischeri possesses a homolog of the Vibrio cholerae transmembrane transcriptional activator ToxR

  1994cited by this paper
• Predicting the Zn(II) Ligands in Metalloproteins: Case Study, Porphobilinogen Synthase

  1993cited by this paper
• Characterization of a periplasmic 3':5'-cyclic nucleotide phosphodiesterase gene, cpdP, from the marine symbiotic bacterium Vibrio fischeri

  1993cited by this paper
• Cyclic AMP in prokaryotes

  1992cited by this paper
• Growth of the marine luminous bacterium Vibrio fischeri on 3′:5′-cyclic AMP: correlation with a periplasmic 3′:5′-cyclic AMP phosphodiesterase

  1992cited by this paper
• A squid that glows in the night: development of an animal-bacterial mutualism

  1992cited by this paper
• Inhibition of enolase: the crystal structures of enolase-Ca2(+)- 2-phosphoglycerate and enolase-Zn2(+)-phosphoglycolate complexes at 2.2-A resolution.

  1992cited by this paper
• UPTAKE, METABOLISM, AND RELEASE OF CAMP IN SELENASTRUM CAPRICORNUTUM (CHLOROPHYCEAE) 1

  1989cited by this paper
• A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR

  1988cited by this paper
• Simple, rapid, and quantitative release of periplasmic proteins by chloroform

  1984cited by this paper
• The zinc-containing high Km cyclic nucleotide phosphodiesterase of bakers' yeast.

  1983cited by this paper
• Purification of two calcium/calmodulin-dependent forms of cyclic nucleotide phosphodiesterase by using conformation-specific monoclonal antibody chromatography.

  1982cited by this paper
• Cellular levels, excretion, and synthesis rates of cyclic AMP in Escherichia coli grown in continuous culture

  1982cited by this paper
• The extracellular cyclic nucleotide phosphodiesterase of Dictyostelium discoideum. Purification and characterization.

  1981cited by this paper
• Dissolved Cycllc Adenosine Monophosphate (CAMP) in the Sea and Uptake of CAMP by Marine Bacteria

  1981cited by this paper
• Binding of inhibitor alters kinetic and physical properties of extracellular cyclic AMP phosphodiesterase from Dictyostelium discoideum.

  1979cited by this paper
• Enzyme structure and mechanism

  1977cited by this paper
• Symbiotic association of Photobacterium fischeri with the marine luminous fish Monocentris japonica; a model of symbiosis based on bacterial studies.

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Stability constant for the zinc-dithiothreitol complex.

  1972cited by this paper
• Stimulation of Intestinal Adenyl Cyclase by Cholera Toxin

  1971cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Cyclic 3',5'-nucleotide phosphodiesterase of Serratia marcescens.

  1970cited by this paper
• Metallo-enzymes released from Escherichia coli by osmotic shock. II. Evidence that 5'-nucleotidase and cyclic phosphodiesterase are zinc metallo-enzymes.

  1968cited by this paper
• Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.

  1962cited by this paper
• Alkaline phosphatase of Escherichia coli: a zinc metalloenzyme.

  1962cited by this paper
• On the evaluation of the constants Vm and KM in enzyme reactions.

  1952cited by this paper
• The extinction coefficients of the reduced band of pyridine nucleotides.

  1948cited by this paper

• Impact of Caffeine on Aquatic Ecosystems: Assessing Trophic-Level Biological Responses

  2025cites this paper
• Ecotoxicological study of six drugs in Aliivibrio fischeri, Daphnia magna and Raphidocelis subcapitata

  2020cites this paper
• The toxicity of angiotensin converting enzyme inhibitors to larvae of the disease vectors Aedes aegypti and Anopheles gambiae

  2017cites this paper
• Identification of the cAMP phosphodiesterase CpdA as novel key player in cAMP‐dependent regulation in Corynebacterium glutamicum

  2017cites this paper
• Assessing protein–ligand binding modes with computational tools: the case of PDE4B

  2017cites this paper
• Rethinking the roles of CRP, cAMP, and sugar-mediated global regulation in the Vibrionaceae

  2016cites this paper
• Dynamics of Dissolved Organic Phosphorus

  2015cites this paper
• Revisiting bacterial cyclic nucleotide phosphodiesterases: cyclic AMP hydrolysis and beyond.

  2015cites this paper
• Growth on glucose decreases cAMP‐CRP activity while paradoxically increasing intracellular cAMP in the light‐organ symbiont Vibrio fischeri

  2015cites this paper
• Cyclic-AMP signalling in Sinorhizobium meliloti and its role in the control of infection during symbiosis with Medicago

  2014cites this paper
• 13 Luminous Bacteria

  2013cites this paper
• Antibacterial activity of di-2-ethylaniline phosphate screened by paper disc diffusion method

  2012cites this paper
• Evolution of self-organisation in Dictyostelia by adaptation of a non-selective phosphodiesterase and a matrix component for regulated cAMP degradation

  2012cites this paper
• The effects of inhibitors and magnesium ions on the activity of the thermostable extracellular cAMP-Specific phosphodiesterase of Physarum polycephalum plasmodium

  2012cites this paper
• Emergence and evolution of the renin–angiotensin–aldosterone system

  2012cites this paper
• Enzymatic and Mutational Analyses of a Class II 3′,5′-Cyclic Nucleotide Phosphodiesterase, PdeE, from Myxococcus xanthus

  2011cites this paper
• Immunogenicity of different cellular fractions of Vibrio parahaemolyticus strains grown under sub-lethal heat and osmotic stress

  2011cites this paper
• Fundamental reaction pathway and free energy profile for hydrolysis of intracellular second messenger adenosine 3',5'-cyclic monophosphate (cAMP) catalyzed by phosphodiesterase-4.

  2011cites this paper
• Characterization of the structures of phosphodiesterase 10 binding with adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate by hybrid quantum mechanical/molecular mechanical calculations.

  2010cites this paper
• Interaction of mimetic analogs of insect kinin neuropeptides with arthropod receptors.

  2010cites this paper
• Agonists/Antagonists of the Insect Kinin and Pyrokinin/PBAN Neuropeptide Classes as Tools for Rational Pest Control

  2009cites this paper
• Theoretical Study on Proton‐Transfer Reaction of Intracellular Second‐messenger 3′,5′‐Cyclic Nucleotide

  2008cites this paper
• Differential 2-D protein gel electrophoresis analysis of Legionella pneumophila wild type and Tat secretion mutants.

  2008cites this paper
• Role of phosphodiesterase 5 in synaptic plasticity and memory

  2008cites this paper
• Genetics of the human renin angiotensin system

  2008cites this paper
• First-principles determination of molecular conformations of cyclic adenosine 3',5'-monophosphate in gas phase and aqueous solution.

  2008cites this paper
• Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.

  2007cites this paper
• Hydrolysis of phosphodiester catalyzed by analogous dinuclear Cu(II) complex in CTAB micellar solution

  2007cites this paper
• Dynamics of Vibrio Populations and Their Role in Environmental Nutrient Cycling

  2006cites this paper
• Structural diversity of angiotensin‐converting enzyme

  2006cites this paper
• Characterization of a catalytic ligand bridging metal ions in phosphodiesterases 4 and 5 by molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations.

  2006cites this paper
• Theoretical study on the ring-opening hydrolysis reaction of cAMP

  2005cites this paper
• Searching for a reliable orientation of ligands in their binding site: comparison between a structure-based (Glide) and a ligand-based (FIGO) approach in the case study of PDE4 inhibitors.

  2005cites this paper
• The EAL Domain Protein VieA Is a Cyclic Diguanylate Phosphodiesterase*

  2005cites this paper
• Studies on the reaction kinetics and the mechanism of hydrolysis of bis(4-nitrophenyl)phosphate (BNPP) by hydroxamic acid complexes containing benzo-15-crown-5

  2005cites this paper
• Marine enzymes.

  2005cites this paper
• Dinuclear macrocyclic polyamine zinc(II) complexes: syntheses, characterization and their interaction with plasmid DNA.

  2005cites this paper
• Chemo-enzymatic synthesis of 2′-O-methoxyethyl ribonucleosides using a phosphodiesterase from Serratia marcescens

  2005cites this paper
• Implications of PDE4 structure on inhibitor selectivity across PDE families

  2004cites this paper
• Proctolin in the post-genomic era: new insights and challenges

  2004cites this paper
• Identification of Angiotensin I in a Cyclostome, Lampetra fluviatilis

  2004cites this paper
• The crystal structure of AMP-bound PDE4 suggests a mechanism for phosphodiesterase catalysis.

  2003cites this paper
• A novel transgenic line of mice exhibiting autosomal recessive male-specific lethality and non-alcoholic fatty liver disease.

  2002cites this paper
• 3′,5′‐Cyclic nucleotide phosphodiesterases class III: Members, structure, and catalytic mechanism

  2002cites this paper
• Effects of heat shock on the thermotolerance, protein composition, and toxin production of Vibrio parahaemolyticus.

  2002cites this paper
• Macrocyclic dinuclear Zn(II) complexes: synthesis, structure and hydrolysis of tris(p-nitrophenyl) phosphate

  2002cites this paper
• Phosphodiester hydrolysis by metal ion macrocyclic dioxotetraamine complexes bearing alcohol pendant in comicellar solution

  2002cites this paper
• Cyclic nucleotide phosphodiesterases: relating structure and function.

  2001cites this paper
• First computational evidence for a catalytic bridging hydroxide ion in a phosphodiesterase active site.

  2001cites this paper
• Characterization of cyclic AMP phosphodiesterases in Leishmania mexicana and purification of a soluble form.

  2000cites this paper
• Atomic structure of PDE4: insights into phosphodiesterase mechanism and specificity.

  2000cites this paper
• Insect Angiotensin‐converting Enzyme: A Processing Enzyme with Broad Substrate Specificity and a Role in Reproduction

  1999cites this paper
• UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase of Escherichia coli is a zinc metalloenzyme.

  1999cites this paper
• Dinuclear lanthanum complex catalyzes the hydrolysis of a phosphate diester with unprecedented speed

  1999cites this paper
• Molecular Recognition of Activated and Unactivated Phosphate Diesters.

  1999cites this paper
• Catalysis of hydrolysis of a phosphate diester by mono- and dinuclear macrocyclic zinc(II) complexes

  1999cites this paper
• Toward a Role for Angiotensin‐Converting Enzyme in Insects

  1998cites this paper
• Angiotensin‐Converting Enzyme and the Metabolism of Regulatory Peptides in Insects

  1997cites this paper
• Lessons from a cooperative, bacterial-animal association: the Vibrio fischeri-Euprymna scolopes light organ symbiosis.

  1996cites this paper
• Cloning and characterisation of angiotensin-converting enzyme from the dipteran species, Haematobia irritans exigua, and its expression in the maturing male reproductive system.

  1996cites this paper
• Separation of proteases: old and new approaches.

  1996cites this paper