Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye.

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Analytical Biochemistry

• Publication date

  2001-02-15

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1006/ABIO.2000.4931PMID 11161325
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Improved cycle sequencing of GC-rich DNA template

  1999cited by this paper
• Improved LM-PCR procedure for in vivo footprinting analysis of GC-rich promoters.

  1999cited by this paper
• Overview of matrix metalloproteinase expression in cultured human cells.

  1998cited by this paper
• Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification.

  1998cited by this paper
• Continuous fluorescent monitoring of rapid cycle polymerase chain reaction

  1998cited by this paper
• Subcycling-PCR for multiplex long-distance amplification of regions with high and low GC content: application to the inversion hotspot in the factor VIII gene.

  1998cited by this paper
• Dimethyl sulfoxide improves RNA amplification.

  1996cited by this paper
• Effect of dimethyl sulfoxide concentration on specificity of primer matching in PCR.

  1992cited by this paper
• Organic solvents as facilitators of polymerase chain reaction.

  1991cited by this paper

• DMSO enhanced one-pot HDA-CRISPR/Cas12a biosensor for ultrasensitive detection of Monkeypox virus.

  2025cites this paper
• Facilitation of Dye-Based Quantitative Real-Time Polymerase Chain Reaction with Poly(ethylene glycol)-Engrafted Graphene Oxide

  2023cites this paper
• PCR enhancers: Types, mechanisms, and applications in long-range PCR.

  2022cites this paper
• Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum.

  2018cites this paper
• Peptide nucleic acid probe-based fluorescence melting curve analysis for rapid screening of common JAK2, MPL, and CALR mutations.

  2017cites this paper
• Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients

  2014cites this paper
• CdTe Quantum Dots Enhance Feasibility of EvaGreen-Based Real-Time PCR with Decent Amplification Fidelity

  2013cites this paper
• A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9.

  2013cites this paper
• The pleiotropic effect of WD-40 domain containing proteins on cellular differentiation and production of secondary metabolites in Streptomyces coelicolor.

  2013cites this paper
• DIMETHYL SULFOXIDE IMPROVES SENSITIVITY AND SPECIFICITY OF RT-PCR AND QRT-PCR AMPLIFICATION OF LOW-EXPRESSED TRANSGENES

  2012influential citation
• Ionic liquids promote PCR amplification of DNA.

  2012cites this paper
• Grundlagen der Polymerasekettenreaktion (PCR) [Biomonitoring Methods in German language, 2004]

  2012cites this paper
• Detection and discrimination of members of the family Luteoviridae by real-time PCR and SYBR® GreenER™ melting curve analysis.

  2011cites this paper
• 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

  2011influential citation
• A rapid simple approach to quantify chromosome conformation capture

  2009cites this paper
• A Convenient Thiazole Orange Fluorescence Assay for the Evaluation of DNA Duplex Hybridization Stability

  2009cites this paper
• SYBR Green I chemistry based Real time PCR: Melting curve discrepancies

  2008cites this paper
• Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I

  2008cites this paper
• Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature

  2007cites this paper
• An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis.

  2006cites this paper
• Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR.

  2006cites this paper
• Cyanine dyes as fluorescent non-covalent labels for nucleic acid research

  2006cites this paper
• Nucleic acid amplification-based techniques for pathogen detection and identification

  2005cites this paper
• Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.

  2005cites this paper
• Quantitative real-time PCR detection of oral Enterococcus faecalis in humans.

  2005cites this paper
• Comparison of SYTO 9 and SYBR Green I for real-time polymerase chain reaction and investigation of the e V ect of dye concentration on ampli W cation and DNA melting curve analysis

  2005influential citation
• DNA-Based Diagnostic Tests for Detection and Identification of Biological Weapons

  2005cites this paper
• Comparison of conventional and real-time RT-PCR for the quantitation of jun protooncogene mRNA and analysis of junB mRNA expression in synovial membranes and isolated synovial fibroblasts from rheumatoid arthritis patients

  2003cites this paper
• mRNA expression of the five membrane‐type matrix metalloproteinases MT1–MT5 in human prostatic cell lines and their down‐regulation in human malignant prostatic tissue

  2003cites this paper
• DNA sequencing of close to 1000 bases in 40 minutes by capillary electrophoresis using dimethyl sulfoxide and urea as denaturants in replaceable linear polyacrylamide solutions

  2002cites this paper