Action of diphenylhydantoin on rhythmicity and contractility of cultured embryonic heart cells.

Chick embryonic heart cells were prepared by trypsin dissociation of embryos eight to nine days in ovo and were grown in a medium containing low potassium (less than 125 mEq/liter). After 24-hour cultivation, the cells were exposed to variable concentrations of diphenylhydantoin, and its effect on contraction rate and contractility of the heart cells was studied. A significant decrease in contraction rate (P <.05) was observed at concentrations of diphenylhydantoin of 25 µg/ml or higher when compared to control. At concentrations of 40 µg/ml or higher, the decrease in rate of contraction was consistently associated with a decrease in contractility of the heart cells. Diphenyihydantoin did not counteract the inotropic and chronotropic effects produced by 10-8 to 10-7 M digoxin nor prevent the toxic effects of 10-8 M digoxin. However, diphenylhydantoin delayed the onset of digoxin-induced cellular arrhythmias.

• Publication year

  1969

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1969-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)28397-5PMID 5351008
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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