Modelling of crowded polymers elucidate effects of double-strand breaks in topological domains of bacterial chromosomes

Using numerical simulations of pairs of long polymeric chains confined in microscopic cylinders, we investigate consequences of double-strand DNA breaks occurring in independent topological domains, such as these constituting bacterial chromosomes. Our simulations show a transition between segregated and mixed state upon linearization of one of the modelled topological domains. Our results explain how chromosomal organization into topological domains can fulfil two opposite conditions: (i) effectively repulse various loops from each other thus promoting chromosome separation and (ii) permit local DNA intermingling when one or more loops are broken and need to be repaired in a process that requires homology search between broken ends and their homologous sequences in closely positioned sister chromatid.

• Publication year

  2013

• Venue

  Nucleic Acids Research

• Publication date

  2013-06-05

• Fields of study

  Biology, Medicine, Materials Science, Physics

• Identifiers
  DOI 10.1093/nar/gkt480PMID 23742906PMCID 3737558
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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