Synthesis of a beta-estradiol-biotin chimera that potently heterodimerizes estrogen receptor and streptavidin proteins in a yeast three-hybrid system.

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Journal of the American Chemical Society

• Publication date

  2003-03-07

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/JA0293305PMID 12656587
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Synthesis of chimeric 7alpha-substituted estradiol derivatives linked to cholesterol and cholesterylamine.

  2002cited by this paper
• In Vivo Protein-Protein Interaction Assays: Beyond Proteins.

  2001cited by this paper
• Structural insights into the mode of action of a pure antiestrogen.

  2001cited by this paper
• Streptavidin-containing chimeric proteins: design and production.

  2000cited by this paper
• A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae.

  2000cited by this paper
• Concepts for the syntheses of biotinylated steroids. Part II: 17beta-estradiol derivatives as immunochemical probes.

  2000cited by this paper
• Novel ligands that function as selective estrogens or antiestrogens for estrogen receptor-alpha or estrogen receptor-beta.

  1999cited by this paper
• Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex.

  1998cited by this paper
• Production of a soluble and functional recombinant streptavidin in Escherichia coli.

  1998cited by this paper
• Controlling protein association and subcellular localization with a synthetic ligand that induces heterodimerization of proteins.

  1996cited by this paper
• A three-hybrid system for detecting small ligand-protein receptor interactions.

  1996cited by this paper
• Yeast Two-hybrid System Demonstrates That Estrogen Receptor Dimerization Is Ligand-dependent in Vivo(*)

  1995cited by this paper
• Controlling signal transduction with synthetic ligands.

  1993cited by this paper
• Genetic dissection of the signaling domain of a mammalian steroid receptor in yeast.

  1992cited by this paper
• Structural requirements for high affinity ligand binding by estrogen receptors: a comparative analysis of truncated and full length estrogen receptors expressed in bacteria, yeast, and mammalian cells.

  1992cited by this paper

• PK/PD modeling of targeted protein degraders: Charting new waters and navigating the shallows.

  2025cites this paper
• Applications of KinTek Explorer Kinetic Simulations of Targeted Protein Degradation for Evaluation and Design of PROTACs.

  2025cites this paper
• Perspective on yeast-3-hybrid assays for target identification of bioactive natural products

  2025cites this paper
• Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibition

  2025cites this paper
• Resource competition shapes CRISPR-mediated gene activation

  2025cites this paper
• Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespan

  2022cites this paper
• A comprehensive kinetic model for ternary complex catalysis

  2022cites this paper
• Arylmethylamino steroids as antiparasitic agents

  2017cites this paper
• Chemical Dimerizers in Three-Hybrid Systems for Small Molecule-Target Protein Profiling.

  2016cites this paper
• Evaluation of activity of an estrogen-derivative as cardioprotector drug using an ischemia-reperfusion injury model.

  2015cites this paper
• Re-engineering protein interfaces yields copper-inducible ferritin cage assembly.

  2013cites this paper
• A comprehensive mathematical model for three-body binding equilibria.

  2013cites this paper
• Protein tango: the toolbox to capture interacting partners.

  2012cites this paper
• Synthetic cell surface receptors for delivery of therapeutics and probes.

  2012cites this paper
• Tools and methodologies capable of isolating and identifying a target molecule for a bioactive compound.

  2012cites this paper
• Proteintango: wie man den Partner einfängt

  2012cites this paper
• Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

  2012cites this paper
• Improving the Yeast Three-Hybrid System for High-Throughput Target Discovery

  2011cites this paper
• Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors

  2011cites this paper
• Exploring a "Nature-Inspired" Strategy for Enhancing the Pharmacology of Small Molecules: Towards the Development of Next Generation Anti-Infectives.

  2011cites this paper
• Identification of a lacosamide binding protein using an affinity bait and chemical reporter strategy: 14-3-3 ζ.

  2011cites this paper
• A Chemical Biology Approach to Discover the Biological Targets of the Antiepileptic Drug Lacosamide

  2010cites this paper
• A coiled-coil enabled split-luciferase three-hybrid system: applied toward profiling inhibitors of protein kinases.

  2010cites this paper
• Evaluation of a novel photoactive and biotinylated dehydroepiandrosterone analog.

  2010cites this paper
• Synthesis and application of a photoaffinity analog of dehydroepiandrosterone (DHEA).

  2010cites this paper
• Probing the topological tolerance of multimeric protein interactions: evaluation of an estrogen/synthetic ligand for FK506 binding protein conjugate.

  2010cites this paper
• Chemical Complementation Assays

  2008cites this paper
• Two‐ and Three‐Hybrid Systems

  2008cites this paper
• Module assembly for protein-surface recognition: geranylgeranyltransferase I bivalent inhibitors for simultaneous targeting of interior and exterior protein surfaces.

  2008cites this paper
• Bisphenol A directly targets tubulin to disrupt spindle organization in embryonic and somatic cells.

  2008cites this paper
• Chemical synthesis and biochemical characterization of a biotinylated derivative of 17beta-estradiol with a long side chain covalently attached to its C-7alpha position.

  2008cites this paper
• Design and Applications of Bifunctional Small Molecules: Why Two Heads Are Better Than One.

  2008cites this paper
• An orthogonal dexamethasone-trimethoprim yeast three-hybrid system.

  2007cites this paper
• 6 Yeast Hybrid Approaches

  2007cites this paper
• A novel and stereoselective synthesis of 7α-alkynylestra-1,3,5(10)-triene-3,17β-estradiol

  2007cites this paper
• Chemical control over protein-protein interactions: beyond inhibitors.

  2007cites this paper
• Synthesis of 7alpha-substituted derivatives of 17beta-estradiol.

  2006cites this paper
• Biological significance and development of practical synthesis of biotin

  2006cites this paper
• Chemical genetics.

  2006cites this paper
• Synthesis of 7α-substituted derivatives of 17β-estradiol

  2006cites this paper
• Synthesis of novel chemical probes for the study of tanshinone binding proteins.

  2006cites this paper
• Steroids: reactions and partial synthesis.

  2005cites this paper
• Finding Cinderella after the ball: a three-hybrid approach to drug target identification.

  2004cites this paper
• Correlation between ligand-receptor affinity and the transcription readout in a yeast three-hybrid system.

  2004cites this paper
• Design, synthesis, and biological evaluation of biotin conjugates of 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid for the isolation of the protein targets.

  2004cites this paper
• Facile synthesis of cids: biotinylated estrone oximes efficiently heterodimerize estrogen receptor and streptavidin proteins in yeast three hybrid systems.

  2004cites this paper
• Induced protein dimerization in vivo through covalent labeling.

  2003cites this paper
• Control of gene expression with small molecules: biotin-mediated acylation of targeted lysine residues in recombinant yeast.

  2003cites this paper