Patch Clamp Study of Neurotransmission at Single Mammalian CNS Synapses

Single mammalian CNS neurons can be acutely isolated with adherent and functional excitatory and inhibitory synaptic nerve terminals (boutons) using a mechanical dissociation procedure without any enzyme treatment (Vorobjev, 1991; Haage & Johansson, 1998; Rhee et al., 1999). This ‘synaptic bouton’ preparation is particularly suitable for physiological and pharmacological investigations of mammalian CNS synaptic transduction mechanisms, and the properties of both the receptors, transporters, and 2nd messengers present in the presynaptic terminals (boutons), and the synaptic and extrasynaptic receptors on the postsynaptic membrane, can be studied.

• Publication year

  2012

• Venue

  Unknown venue

• Publication date

  2012-03-23

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.5772/38076
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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