Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones

Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti- idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.

• Publication year

  1982

• Venue

  Journal of Experimental Medicine

• Publication date

  1982-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.155.4.1100PMID 6174671PMCID 2186651
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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