Effects of chitosan on healing and strength of colonic anastomosis in rats.

PURPOSE To investigate whether chitosan application over colonic anastomosis line, provide reinforcement, and subsequently improve anastomotic healing. METHODS Forty eight Wistar albino female rats were used and were randomly divided into four groups, 12 rats in each: The control groups (1 and 3) received no further treatment. The experimental groups (2 and 4) received chitosan application over the colonic anastomosis. After sacrifying rats at the end of the experiment (either on day three or on day seven, depending on the group), colonic bursting pressure, a hihydroxyproline level and histopathologic characteristics of the perianastomotic tissue were examined. RESULTS At three days, chitosan and control groups had similar values for histopathologically. On day seven, chitosan group had significantly higher mean score of collagenization (p=0.007) and a significantly higher bursting pressure (p=0.038). CONCLUSION Our study emphasizes the positive effect of chitosan in the process of collagenation in colonic anastomosis healing.

• Publication year

  2012

• Venue

  Acta Cirurgica Brasileira

• Publication date

  2012-10-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1590/S0102-86502012001000007PMID 23033132
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Stapled versus handsewn methods for ileocolic anastomoses.

  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
• [Effect of carboxymethylchitosan-carboxymethylcellulose film on colonic anastomosis healing].

  2009cited by this paper
• Effect of 2-Octyl-Cyanoacrylate on Ischemic Anastomosis of the Left Colon

  2009cited by this paper
• [Scaffolds based on collagen and chitosan for post-burn tissue engineering].

  2008cited by this paper
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  2008cited by this paper
• Review Paper: Chitosan Derivatives as Promising Materials for Controlled Drug Delivery

  2008cited by this paper
• Preventive effect of gelatinizedly-modified chitosan film on peritoneal adhesion of different types.

  2007cited by this paper
• Preventive effects of chitosan on peritoneal adhesion in rats.

  2006cited by this paper
• Action of matrix metalloproteinases at restricted sites in colon anastomosis repair: an immunohistochemical and biochemical study.

  2006cited by this paper
• Chitin and chitosan: Properties and applications

  2006cited by this paper
• An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor.

  2006influential reference
• Chitosan hydrogel as a drug delivery carrier to control angiogenesis

  2006cited by this paper
• Healing of colon anastomoses covered with fibrin glue after immediate postoperative intraperitoneal administration of 5-fluorouracil

  2004cited by this paper
• The effect of tissue adhesive, octyl-cyanoacrylate, on the healing of experimental high-risk and normal colonic anastomoses.

  2004cited by this paper
• Controlled release of fibroblast growth factors and heparin from photocrosslinked chitosan hydrogels and subsequent effect on in vivo vascularization.

  2003cited by this paper
• Chitosan inhibits prostaglandin E2 formation and cyclooxygenase-2 induction in lipopolysaccharide-treated RAW 264.7 macrophages.

  2003cited by this paper
• Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process.

  2002cited by this paper
• An investigation on skin wound healing in mice with a taurine-chitosan gel formulation

  2002cited by this paper
• Water-soluble chitosan inhibits the production of pro-inflammatory cytokine in human astrocytoma cells activated by amyloid beta peptide and interleukin-1beta.

  2002cited by this paper
• Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel

  2001cited by this paper
• Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages.

  2001cited by this paper
• Less reversal of liver fibrosis after prolonged carbon tetrachloride injection.

  2001cited by this paper
• Effect of matrix metalloproteinase inhibition on colonic anastomotic healing in rats

  2001cited by this paper
• Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs.

  1999cited by this paper
• Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro.

  1997cited by this paper
• Use of chitosan as a biomaterial: studies on its safety and hemostatic potential.

  1997cited by this paper
• Healing in the gastrointestinal tract.

  1997cited by this paper
• Effects of chronic corticosteroids and vitamin A on the healing of intestinal anastomoses.

  1992cited by this paper
• Effect of antibiotics in fibrin sealant on healing colonic anastomoses in the rat

  1992cited by this paper
• Enhancement of wound healing by topical treatment with epidermal growth factor.

  1989cited by this paper
• The healing of colonic anastomoses after early intraperitoneal chemotherapy: an experimental study in rats.

  1988cited by this paper
• Two Improved and Simplified Methods for the Spectrophotometric Determination of Hydroxyproline.

  1963cited by this paper

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  2025cites this paper
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  2025cites this paper
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  2024cites this paper
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  2023cites this paper
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  2023cites this paper
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  2021cites this paper
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  2021cites this paper
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  2021cites this paper
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  2018cites this paper
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  2016cites this paper
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  2016cites this paper
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  2015cites this paper
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  2014cites this paper
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  2013cites this paper