Grape seed proanthocyanidin extract protects human lens epithelial cells from oxidative stress via reducing NF-кB and MAPK protein expression

Purpose Oxidative damage induced by H2O2 treatment can irreversibly damage the lens epithelium, resulting in cell death and cataract. Grape seed extract (GSE) is a widely consumed dietary supplement that has the capability to scavenge oxidants and free radicals. GSE contain 70%–95% standardized proanthocyanidins. The study described herein investigated the protective effect of Grape seed proanthocyanidin extract (GSPE) on H2O2-induced oxidative stress in human lens epithelial B-3 (HLEB-3) cells and the possible molecular mechanism involved. Methods HLE-B3 cells exposed to different doses of H2O2 were cultured with various concentrations of GSPE and subsequently monitored for cell viability by the 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis rate and ROS generation were detected by flow cytometric analysis. Expression of NF-кB/P65 and mitogen activated protein kinase (MAPK) proteins were measured by western blot. Results GSPE clearly reduced H2O2 induced cell apoptosis and reactive oxygen species (ROS) generation and protected HLEB-3 cells from H2O2 induced oxidative damage. GSPE depressed H2O2-induced activation and translocation of NF-кB/p65. GSPE also depressed H2O2-induced phosphorylation of the p38 and c-Jun N-terminal kinase (JNK) proteins of the MAPK family at various time points studied. Conclusions GSPE could be useful in attenuation of H2O2-induced oxidative stress and the activation of NF-кB and MAPK signaling in HLE-B3 cells, which suggests that GSPE has a potential protective effect against cataractogenesis.

• Publication year

  2011

• Venue

  Molecular Vision

• Publication date

  2011-01-20

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  PMID 21264233PMCID 3025097
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract.

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  2009cited by this paper
• [Study on preventing effects of procyanidins on selenite cataract development in rats].

  2008cited by this paper
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  2008cited by this paper
• Effect of grape polyphenols on oxidative stress in canine lens epithelial cells.

  2008cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2005cited by this paper
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  2005cited by this paper
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  2005cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2001cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  2000cited by this paper
• Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention.

  2000cited by this paper
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  1997cited by this paper
• Oxidative stress‐induced cataract: mechanism of action

  1995cited by this paper
• Propagation and immortalization of human lens epithelial cells in culture.

  1994cited by this paper
• Nuclear factor kappa B: an oxidative stress-responsive transcription factor of eukaryotic cells (a review).

  1992cited by this paper
• Nuclear Factor Kb: An Oxidative Stress-Responsive Transcription Factor of Eukaryotic Cells (A Review)

  1992cited by this paper
• The conquest of cataract: a global challenge

  1985cited by this paper
• Bowman lecture. The conquest of cataract: a global challenge.

  1985cited by this paper

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