Possible involvement of protein kinase C in proliferation and differentiation of osteoblast‐like cells

In cloned osteoblast‐like cells, MC3T3‐E1, 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), a protein kinase C activating phorbol ester, and 1‐oleoyl‐2‐acetylglycerol (OAG), a specific activator for protein kinase C, stimulated DNA synthesis in a dose‐dependent manner. Both TPA and OAG acted synergistically with insulin‐like growth factor I to stimulate DNA synthesis. TPA as well as OAG suppressed the increase in alkaline phosphatase activity of MC3T3‐E1 cells induced by parathyroid hormone. These results suggest that protein kinase C is involved in the process which directs osteoblast‐like cells toward proliferation.

• Publication year

  1989

• Venue

  FEBS Letters

• Publication date

  1989-01-30

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/0014-5793(89)80126-7PMID 2917645
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Possible involvement of protein kinase C in platelet-derived growth factor-stimulated DNA synthesis in vascular smooth muscle cells.

  1987cited by this paper
• Early signals in the mitogenic response.

  1986cited by this paper
• Studies and perspectives of protein kinase C.

  1986cited by this paper
• Induction of protein kinase C activation and Ca2+ mobilization by fibroblast growth factor in Swiss 3T3 cells

  1985cited by this paper
• The PDGF-inducible 'competence genes': intracellular mediators of the mitogenic response.

  1985cited by this paper
• Membrane phospholipid metabolism and signal transduction for protein phosphorylation.

  1984cited by this paper
• Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells.

  1984cited by this paper
• In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria

  1983cited by this paper
• Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells

  1983cited by this paper
• Establishment of a clonal osteogenic cell line from newborn mouse calvaria

  1981cited by this paper
• Functional properties of hormonally responsive cultured normal and malignant rat osteoblastic cells.

  1981cited by this paper
• Parathyroid hormone-responsive clonal cell lines from rat osteosarcoma.

  1980cited by this paper
• Effect of insulinlike growth factor I on DNA and protein synthesis in cultured rat calvaria.

  1980cited by this paper
• Phorbol esters and vasopressin stimulate DNA synthesis by a common mechanism

  1980cited by this paper
• Dual control of cell growth by somatomedins and platelet-derived growth factor.

  1979cited by this paper
• The quantitative histochemistry of brain. II. Enzyme measurements.

  1954cited by this paper

• Chitinase 3-like 1 expression by human (MG63) osteoblasts in response to lysophosphatidic acid and 1,25-dihydroxyvitamin D3.

  2016cites this paper
• Costunolide stimulates the function of osteoblastic MC3T3-E1 cells.

  2011cites this paper
• Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells.

  2009cites this paper
• In vitro Evaluation of Potential Calcium Phosphate Scaffolds for Tissue Engineering

  2006cites this paper
• Vanadium and bone development: putative signaling pathways.

  2006cites this paper
• Involvement of p44/p42 MAP kinase in insulin-like growth factor-I-induced alkaline phosphatase activity in osteoblast-like-MC3T3-E1 cells.

  2006cites this paper
• Possible Involvement of Phosphatidylinositol 3-Kinase/Akt Pathway in Insulin-like Growth Factor-I-induced Alkaline Phosphatase Activity in Osteoblasts

  2005cites this paper
• Involvement of phosphatidylcholine hydrolysis by phospholipase C in prostaglandin F2Α-induced 1,2-diacylglycerol formation in osteoblast-like MC3T3-E1 cells

  2004cites this paper
• Stimulatory effect of prostaglandin F(2alpha) on Na-dependent phosphate transport in osteoblast-like cells.

  2003cites this paper
• [Heat shock protein 27 in osteoblasts].

  2002cites this paper
• 骨代謝とストレスタンパク質27（heat shock protein 27: HSP27）

  2002cites this paper
• Osteoblast responses to as-deposited and heat treated sputtered CaP surfaces

  2001cites this paper
• Phosphatidylinositol 3-kinase translocates to the nucleus of osteoblast-like MC3T3-E1 cells in response to insulin-like growth factor I and platelet-derived growth factor but not to the proapoptotic cytokine tumor necrosis factor alpha.

  2000cites this paper
• Phosphatidylinositol 3‐Kinase Translocates to the Nucleus of Osteoblast‐Like MC3T3‐E1 Cells in Response to Insulin‐Like Growth Factor I and Platelet‐Derived Growth Factor But Not to the Proapoptotic Cytokine Tumor Necrosis Factor α

  2000cites this paper
• Effect of transforming growth factor-beta on osteoblast cells cultured on 3 different hydroxyapatite surfaces.

  1999cites this paper
• Osteoblast precursor cell activity on HA surfaces of different treatments.

  1998cites this paper
• Osteoblast cell response to hydroxyapatite of different heat treatments

  1997cites this paper
• Prostaglandin F2alpha stimulates interleukin-6 synthesis via activation of PKC in osteoblast-like cells.

  1997cites this paper
• Protein kinase C activation inhibits stress‐induced synthesis of heat shock protein 27 in osteoblast‐like cells: Function of arachidonic acid

  1996cites this paper
• Protein kinase C activation inhibits stress-induced synthesis of heat shock protein 27 in osteoblast-like cells: function of arachidonic acid.

  1996cites this paper
• Okadaic acid enhances prostaglandin E1-induced alkaline phosphatase activity in osteoblast-like cells: regulation at a point downstream from protein kinase A.

  1996cites this paper
• Function of Ca2+ in phosphatidylcholine-hydrolyzing phospholipase D activation in osteoblast-like cells.

  1996cites this paper
• Thrombin induces proliferation of osteoblast‐like cells through phosphatidylcholine hydrolysis

  1996cites this paper
• Basic fibroblast growth factor stimulates phosphatidylcholine‐hydrolyzing phospholipase D in osteoblast‐like cells

  1996cites this paper
• Function of Ca2+ influx in phospholipase D activation induced by prostaglandin F2 alpha in osteoblast-like cells: involvement of tyrosine kinase.

  1995cites this paper
• Effect of platelet-derived growth factor on phosphatidylcholine-hydrolyzing phospholipase D in osteoblast-like cells.

  1995cites this paper
• Mechanism of phospholipase D activation induced by prostaglandin D2 in osteoblast-like cells: function of Ca2+/calmodulin.

  1995cites this paper
• Tyrosine kinase regulates phospholipase D activation at a point downstream from protein kinase C in osteoblast‐like cells

  1995cites this paper
• Mechanism of phospholipase D activation induced by extracellular ATP in osteoblast-like cells.

  1995cites this paper
• Effect of prostaglandin E2 on phospholipase D activity in osteoblast‐like MC3T3‐E1 cells

  1995cites this paper
• Thromboxane A2-stimulated phospholipase D in osteoblast-like cells: possible involvement of PKC.

  1995cites this paper
• Prostaglandin F2α activates phospholipase D independently from activation of protein kinase C in osteoblast‐like cells

  1994cites this paper
• Effect of prostaglandin F2α on Ca2+ influx in osteoblast‐like cells: Function of tyrosine kinase

  1994cites this paper
• Effect of vitamin D3 on prostaglandin E2 synthesis in osteoblast-like cells.

  1994cites this paper
• Prostaglandin F2 alpha activates phospholipase D independently from activation of protein kinase C in osteoblast-like cells.

  1994cites this paper
• Okadaic acid reverses the inhibitory effect of protein kinase C on alkaline phosphatase activity in osteoblast-like cells.

  1994cites this paper
• Effect of endothelin-1 on phospholipase D activity in osteoblast-like cells.

  1994cites this paper
• Pertussis toxin enhances fetal calf serum-induced alkaline phosphatase activity in osteoblast-like cells.

  1994cites this paper
• Hypoxia impairs cellular differentiation.

  1994cites this paper
• Chronic hypoxia impairs the differentiation of 3T3‐L1 fibroblast in culture: Role of sustained protein kinase C activation

  1994cites this paper
• Effect of prostaglandin F2 alpha on Ca2+ influx in osteoblast-like cells: function of tyrosine kinase.

  1994cites this paper
• Effects of glucocorticoid on signalling by prostaglandin E2 in osteoblast-like cells.

  1993cites this paper
• Triiodothyronine stimulates45Ca-accumulation via synthesis of insulin-like growth factor-I in cultures of osteoblast-like cells

  1993cites this paper
• Protein kinase C activation amplifies prostaglandin F2 alpha-induced prostaglandin E2 synthesis in osteoblast-like cells.

  1992cites this paper
• Proliferative effect of PGD2 on osteoblast-like cells; independent activation of pertussis toxin-sensitive GTP-binding protein from PGE2 or PGF2 alpha.

  1992cites this paper
• Hypoxia-mediated impaired differentiation by LLC-PK1 cells: evidence based on the protein kinase C profile.

  1992cites this paper
• Activation of protein kinase C inhibits 45Ca-accumulation in cultures of osteoblast-like cells: possible involvement of insulin-like growth factor-I.

  1992cites this paper
• The existence of distinct classes of prostaglandin E2 receptors mediating adenylate cyclase and phospholipase C pathways in osteoblastic clone MC3T3-E1.

  1992cites this paper
• Different protein kinase C isozymes could modulate bradykinin-induced extracellular calcium-dependent and -independent increases in osteoblast-like MC3T3-E1 cells.

  1992cites this paper
• Autoregulation of prostaglandin E2-induced Ca2+ influx in osteoblast-like cells: inhibition by self-induced activation of protein kinase C.

  1992cites this paper
• Proliferative effect of PGD[2] on osteoblast-like cells : independent activation of pertussis toxin-sensitive GTP-binding protein from PGE[2] or PGF[2]α

  1992cites this paper
• Protein kinase C activation amplifies prostaglandin F2α‐induced prostaglandin E2 synthesis in osteoblast‐like cells

  1992cites this paper
• 4 – BONE GROWTH

  1992cites this paper
• The protein kinase-C activation domain of the parathyroid hormone.

  1992cites this paper
• Effects of hypergravity on proliferation and differentiation of osteoblast-like cells.

  1991cites this paper
• Cellular activity of osteoblasts in the presence of hydroxyapatite: an in vitro experiment.

  1991cites this paper
• Parathyroid hormone fragment [3-34] stimulates protein kinase C (PKC) activity in rat osteosarcoma and murine T-lymphoma cells.

  1990cites this paper
• Insulin-like growth factor-I stimulates45Ca-accumulation in cultures of osteoblast-like cells

  1990cites this paper