Genetic Diversity and mRNA Expression of Porcine MHC Class I Chain-Related 2 (SLA-MIC2) Gene and Development of a High-Resolution Typing Method

The genetic structure and function of MHC class I chain-related (MIC) genes in the pig genome have not been well characterized, and show discordance in available data. Therefore, we have experimentally characterized the exon-intron structure and functional copy expression pattern of the pig MIC gene, SLA-MIC2. We have also studied the genetic diversity of SLA-MIC2 from seven different breeds using a high-resolution genomic sequence-based typing (GSBT) method. Our results showed that the SLA-MIC2 gene has a similar molecular organization as the human and cattle orthologs, and is expressed in only a few tissues including the small intestine, lung, and heart. A total of fifteen SLA-MIC2 alleles were identified from typing 145 animals, ten of which were previously unreported. Our analysis showed that the previously reported and tentatively named SLA-MIC2*05, 07, and 01 alleles occurred most frequently. The observed heterozygosity varied from 0.26 to 0.73 among breeds. The number of alleles of the SLA-MIC2 gene in pigs is somewhat lower compared to the number of alleles of the porcine MHC class I and II genes; however, the level of heterozygosity was similar. Our results indicate the comprehensiveness of using genomic DNA-based typing for the systemic study of the SLA-MIC2 gene. The method developed for this study, as well as the detailed information that was obtained, could serve as fundamental tools for understanding the influence of the SLA-MIC2 gene on porcine immune responses.

• Publication year

  2015

• Venue

  PLoS ONE

• Publication date

  2015-08-25

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0135922PMID 26305091PMCID 4549063
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

  2013cited by this paper
• Systematic analysis of swine leukocyte antigen-DRB1 nucleotide polymorphisms using genomic DNA-based high-resolution genotyping and identification of new alleles.

  2011cited by this paper
• Protein Native-State Stabilization by Placing Aromatic Side Chains in N-Glycosylated Reverse Turns

  2011cited by this paper
• Simple and comprehensive SLA-DQB1 genotyping using genomic PCR and direct sequencing.

  2010cited by this paper
• The core trisaccharide of an N-linked glycoprotein intrinsically accelerates folding and enhances stability

  2009cited by this paper
• Identification of Epitopes and Immunodominant Regions on the MICA Protein Defined by Alloantibodies From Kidney Transplant Patients

  2009cited by this paper
• Diversification of porcine MHC class II genes: evidence for selective advantage

  2009cited by this paper
• MHC class I chain-related protein A antibodies and shedding are associated with the progression of multiple myeloma

  2008cited by this paper
• Genomic location and characterisation of MIC genes in cattle

  2008cited by this paper
• Regulation of porcine classical and nonclassical MHC class I expression

  2007cited by this paper
• Polymorphism of the transmembrane region of the MICA gene and human brucellosis.

  2007cited by this paper
• In Vivo Expression Pattern of MICA and MICB and Its Relevance to Auto-Immunity and Cancer

  2007cited by this paper
• The genomic sequence and analysis of the swine major histocompatibility complex.

  2006cited by this paper
• Use of competitive exclusion to control enterotoxigenic strains of Escherichia coli in weaned pigs,,

  2005cited by this paper
• Natural selection on the peptide-binding regions of major histocompatibility complex molecules

  2004cited by this paper
• Human MHC class I chain related (MIC) genes: their biological function and relevance to disease and transplantation.

  2004cited by this paper
• Nucleotide sequence of a human MHC class IMICB cDNA

  2004cited by this paper
• Extended human leukocyte antigen haplotype EH18.1 influences progression to hepatocellular carcinoma in patients with hepatitis C virus infection.

  2004cited by this paper
• The Phylogenetic History of the MHC Class I Gene Families in Pig, Including a Fossil Gene Predating Mammalian Radiation

  2003cited by this paper
• SWINE 2000, PART II: REFERENCE OF SWINE HEALTH AND HEALTH MANAGEMENT IN THE UNITED STATES, 2000

  2002cited by this paper
• Binding of Escherichia coli adhesin AfaE to CD55 triggers cell-surface expression of the MHC class I-related molecule MICA

  2002cited by this paper
• MHC heterozygosity confers a selective advantage against multiple-strain infections

  2002cited by this paper
• Association of the MIC-A gene and HLA-B51 with Behçet's disease in Arabs and non-Ashkenazi Jews in Israel

  2002cited by this paper
• MIC expression in renal and pancreatic allografts.

  2002cited by this paper
• Activation‐induced expression of MICA on T lymphocytes involves engagement of CD3 and CD28

  2002cited by this paper
• Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells.

  2001cited by this paper
• Sequence of the swine major histocompatibility complex region containing all non-classical class I genes.

  2001cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• MIC genes: from genetics to biology.

  2000cited by this paper
• Relationship between the occurrence of cysteine in proteins and the complexity of organisms.

  2000cited by this paper
• BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT

  1999cited by this paper
• Differential surface expression of MICA by endothelial cells, fibroblasts, keratinocytes, and monocytes.

  1999cited by this paper
• Spatial arrangement of pig MHC class I sequences

  1999cited by this paper
• Broad tumor-associated expression and recognition by tumor-derived γδ T cells of MICA and MICB

  1999cited by this paper
• Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.

  1999cited by this paper
• Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB.

  1999cited by this paper
• MIC-A allele profiles and HLA class I associations in Behçet's disease

  1999cited by this paper
• The major histocompatibility complex in swine

  1999cited by this paper
• An activating immunoreceptor complex formed by NKG2D and DAP10.

  1999cited by this paper
• Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.

  1998cited by this paper
• MICA, a new polymorphic HLA-related antigen, is expressed mainly by keratinocytes, endothelial cells, and monocytes

  1997cited by this paper
• Population genetic analysis of codominant and dominant markers and quantitative traits.

  1997cited by this paper
• Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium.

  1996influential reference
• A second lineage of mammalian major histocompatibility complex class I genes.

  1994influential reference
• HLA and disease associations: detecting the strongest association.

  1994cited by this paper
• CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

  1994influential reference
• Evolutionary Genetics of the Major Histocompatibility Complex

  1994cited by this paper
• Biological roles of oligosaccharides: all of the theories are correct

  1993cited by this paper
• The neighbor-joining method: a new method for reconstructing phylogenetic trees.

  1987cited by this paper
• A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences

  1980cited by this paper

• The pig is a better model than the rabbit or rat for studying the pathophysiology of human mesenteric arteries.

  2023cites this paper
• Genomic consequences of a century of inbreeding and isolation in the Danish wild boar population

  2022cites this paper
• Development of a simple SLA-1 copy-number-variation typing and the comparison of typing accuracy between real-time quantitative and droplet digital PCR.

  2019cites this paper