Germline mutagenesis mediated by Sleeping Beauty transposon system in mice

Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice. Initially, the transposition efficiency was found to be low in cultured systems, but its activity in germ cells was unexpectedly high. This difference in transposition efficiency was found to be largely dependent on chromosomal status of the host genomic DNA and transposon vector design. The SB transposon system has been found to be suitable for comprehensive mutagenesis in mice. Therefore, it is an effective tool as a forward genetics screen for tagged insertional mutagenesis in mice.

• Publication year

  2007

• Venue

  Genome Biology

• Publication date

  2007-10-31

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/gb-2007-8-s1-s14PMID 18047691PMCID 2106844
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Multiconstrained gene clustering based on generalized projections

  2010cited by this paper
• Gene Mutations and Genomic Rearrangements in the Mouse as a Result of Transposon Mobilization from Chromosomal Concatemers

  2006cited by this paper
• Sleeping Beauty Transposase Has an Affinity for Heterochromatin Conformation

  2006cited by this paper
• Harnessing a High Cargo-Capacity Transposon for Genetic Applications in Vertebrates

  2006cited by this paper
• Thin films of Type 1 collagen for cell by cell analysis of morphology and tenascin-C promoter activity

  2006cited by this paper
• Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice.

  2005cited by this paper
• Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system

  2005cited by this paper
• Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse

  2005cited by this paper
• Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells

  2005cited by this paper
• Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein

  2005cited by this paper
• Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system

  2005influential reference
• Transposition of hAT elements links transposable elements and V(D)J recombination

  2004cited by this paper
• Enhancement of Sleeping Beauty Transposition by CpG Methylation: Possible Role of Heterochromatin Formation

  2004influential reference
• A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium

  2004cited by this paper
• Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells.

  2004cited by this paper
• Characterization of Sleeping Beauty Transposition and Its Application to Genetic Screening in Mice

  2003influential reference
• Gene transfer into genomes of human cells by the sleeping beauty transposon system.

  2003cited by this paper
• Technicolour transgenics: imaging tools for functional genomics in the mouse

  2003cited by this paper
• Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal

  2002cited by this paper
• Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice

  2001influential reference
• Transposition and gene disruption in the male germline of the mouse

  2001cited by this paper
• Regulated transposition of a fish transposon in the mouse germ line

  2001cited by this paper
• Mobility assays confirm the broad host‐range activity of the Minos transposable element and validate new transformation tools

  2000cited by this paper
• Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells.

  1998cited by this paper
• Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system

  1998cited by this paper
• DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations.

  1998cited by this paper
• Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells.

  1997cited by this paper
• Molecular Medicine

  1986cited by this paper

• Efficient generation of epigenetic disease model mice by epigenome editing using the piggyBac transposon system

  2022cites this paper
• Genetic analysis of synaptogenesis

  2020cites this paper
• HMGXB4 Targets Sleeping Beauty Transposition to Vertebrate Germinal Stem Cells

  2020cites this paper
• Clinical relationships between the rs2212020 and rs189897 polymorphisms of the ITGA9 gene and epithelial ovarian cancer

  2019cites this paper
• Title piggyBac transposon-mediated long-term gene expression in mice

  2019cites this paper
• Mapping Viral Susceptibility Loci in Mice.

  2019cites this paper
• Giving the Genes a Shuffle: Using Natural Variation to Understand Host Genetic Contributions to Viral Infections

  2018cites this paper
• High-throughput mouse phenomics for characterizing mammalian gene function

  2018cites this paper
• Protocadherin-αC2 is required for diffuse projections of serotonergic axons

  2017cites this paper
• PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice

  2016cites this paper
• Mutations and Experimental Mutagenesis

  2015cites this paper
• Development and Use of Mouse Models of Atherosclerosis

  2015cites this paper
• Analysis of the host response to influenza A virus infection in mouse genetic reference populations

  2015cites this paper
• A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos

  2015cites this paper
• Genetics of the Mouse

  2014cites this paper
• Physiology of the read–write genome

  2014cites this paper
• PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are susceptible to porcine reproductive and respiratory syndrome virus.

  2013cites this paper
• The Mammalian Phenotype Ontology as a unifying standard for experimental and high-throughput phenotyping data

  2012cites this paper
• Retake the center stage--new development of rat genetics.

  2012cites this paper
• Forward genetic screens in Xenopus using transposon-mediated insertional mutagenesis.

  2012cites this paper
• Creating a "hopeful monster": mouse forward genetic screens.

  2011cites this paper
• Use of Transposon-Transposase Systems for Stable Genetic Modification of Embryonic Stem Cells

  2011cites this paper
• Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis

  2011cites this paper
• Genetic manipulations in the rat: progress and prospects

  2011cites this paper
• Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation.

  2010cites this paper
• Functional analysis of the mospd gene family

  2010cites this paper
• piggyBac Transposon-mediated Long-term Gene Expression in Mice

  2010cites this paper
• Site-directed integration of transgenes: transposons revisited using DNA-binding-domain technologies

  2010cites this paper
• Système de transposons d'adn de plie

  2009cites this paper
• Total Expression and Dual Gene-regulatory Mechanisms Maintained in Deletions and Duplications of the Pcdha Cluster*

  2009cites this paper
• Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish

  2008cites this paper
• Mouse Forward Genetics in the Study of the Peripheral Nervous System and Human Peripheral Neuropathy

  2008cites this paper
• Transposon–Host Cell Interactions in the Regulation of Sleeping Beauty Transposition

  2008cites this paper
• Protein kinase D (PKD) mouse models – towards an understanding of the physiological role of PKD

  2008cites this paper
• Journal of Reproduction and Development

  year unknowncites this paper