Thrombin induces serotonin secretion and aggregation independently of inositol phospholipids hydrolysis and protein phosphorylation in human platelets permeabilized with saponin.

We have observed that the addition of Ca2+ to platelets, permeabilized with saponin, promotes a drastic dephosphorylation of proteins and polyphosphoinositides without inducing platelet responses. Subsequent addition of thrombin could promote secretion of serotonin and aggregation in the absence of phospholipase C-induced breakdown of the inositol phospholipids and protein phosphorylation. This information indicates that activation of saponized platelets by thrombin is independent of the formation of second messengers derived from the phospholipase C-induced breakdown of the inositol phospholipids. The implications of this result for intact platelets are discussed.

• Publication year

  1985

• Venue

  Journal of Biological Chemistry

• Publication date

  1985-06-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)88890-1PMID 2987242
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The rapid formation of inositol phosphates in human platelets by thrombin is inhibited by prostacyclin.

  1984cited by this paper
• Cyclic nucleotides control a system which regulates Ca2+ sensitivity of platelet secretion

  1984cited by this paper
• Potentiation by thrombin of the secretion of serotonin from permeabilized platelets equilibrated with Ca2+ buffers. Relationship to protein phosphorylation and diacylglycerol formation.

  1984cited by this paper
• myo-Inositol 1,4,5-trisphosphate stimulates protein phosphorylation in saponin-permeabilized human platelets.

  1984cited by this paper
• Ionomycin can elevate intraplatelet Ca2+ and activate phospholipase A without activating phospholipase C.

  1984cited by this paper
• Inositol trisphosphate and diacylglycerol as second messengers.

  1984cited by this paper
• Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol.

  1983cited by this paper
• Diacylglycerol and phorbol ester stimulate secretion without raising cytoplasmic free calcium in human platelets

  1983cited by this paper
• Calcium, phospholipid turnover and transmembrane signalling.

  1983cited by this paper
• Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets.

  1982cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Serotonin in Platelets

  2019cites this paper
• Dual roles for polo-like kinase 3 in the regulation of platelet function

  2013cites this paper
• Transfection of Human Platelets with Short Interfering RNA

  2011cites this paper
• Phosphatidylinositol 4,5-bisphosphate mediates Ca2+-induced platelet alpha-granule secretion: evidence for type II phosphatidylinositol 5-phosphate 4-kinase function.

  2001cites this paper
• Phosphatidylinositol 4,5-Bisphosphate Mediates Ca2+-induced Platelet α-Granule Secretion

  2001cites this paper
• Hyperactive phosphoinositide signaling pathway in platelets of depressed patients: effect of desipramine treatment.

  2001cites this paper
• Localization by Segmental Deletion Analysis and Functional Characterization of a Third Actin-binding Site in Domain 5 of Scinderin*

  1998cites this paper
• Action of antitumoral platinum complexes on in vitro platelet functions.

  1998cites this paper
• Cellular proliferation and second messenger formation altered by dialysis membranes.

  1997cites this paper
• Analysis of GTP-binding proteins, phosphoproteins, and cytosolic calcium in functional heterogeneous human blood platelet subpopulations.

  1997cites this paper
• Inhibitory Effect of Prostacyclin and Nitroprusside on Type IIB von Willebrand Factor-promoted Platelet Activation

  1996cites this paper
• Correlation between cytosolic Ca2+ concentration, protein phosphorylation and platelet secretion.

  1996cites this paper
• Recombinant scinderin, an F-actin severing protein, increases calcium-induced release of serotonin from permeabilized platelets, an effect blocked by two scinderin-derived actin-binding peptides and phosphatidylinositol 4,5-bisphosphate.

  1996cites this paper
• Type IIB von Willebrand factor induces phospholipase A2 activation and cytosolic Ca2+ increase in platelets.

  1995cites this paper
• Effect of non-saponin fraction from Panax ginseng on cGMP and thromboxane A2 in human platelet aggregation.

  1995cites this paper
• Dissociation between secretion and protein phosphorylation in agonist-stimulated platelets; action of PCA-4230, a new antithrombotic drug.

  1994cites this paper
• Staurosporine-independent platelet aggregation induced by the calcium ionophore ionomycin is inhibited by prostacyclin and sodium nitroprusside and stimulated by adrenaline.

  1993cites this paper
• The involvement of the cytoskeleton in regulating IP3 receptor‐mediated internal Ca2+ release in human blood platelets.

  1993cites this paper
• Urokinase-urokinase receptor interaction: non-mitogenic signal transduction in human epidermal cells.

  1993cites this paper
• Interaction between antidepressants and phosphoinositide signal transduction system in human platelets.

  1993cites this paper
• Defective signal transduction in platelets from cirrhotics is associated with increased cyclic nucleotides.

  1993cites this paper
• Suppression by wortmannin of platelet responses to stimuli due to inhibition of pleckstrin phosphorylation.

  1992cites this paper
• Mastoparan promotes exocytosis and increases intracellular cyclic AMP in human platelets. Evidence for the existence of a Ge-like mechanism of secretion.

  1992cites this paper
• Inactivation of platelet-activating factor by a putative acetylhydrolase from the gastrointestinal nematode parasite Nippostrongylus brasiliensis.

  1992cites this paper
• Reduced functions of intracellular Ca2+ in aggregation, secretion and protein phosphorylation of permeabilized platelets from stroke-prone spontaneously hypertensive rats.

  1992cites this paper
• Platelet aggregation and dense granule secretion in a colony of dogs with spontaneous hypertension

  1992cites this paper
• Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1).

  1991cites this paper
• Deletion or substitution within the alpha platelet-derived growth factor receptor kinase insert domain: effects on functional coupling with intracellular signaling pathways

  1991cites this paper
• Platelet responses promoted by the activation of protein kinase C or the increase of cytosolic Ca2+ are potentiated by adrenaline. Effects of cAMP and staurosporine.

  1991cites this paper
• Heparin inhibits A431 cell growth independently of serum and EGF mitogenic signalling

  1991cites this paper
• Mitogenic signal transduction in normal and transformed 32D hematopoietic cells

  1991cites this paper
• Lipoxin A4 inhibits phosphoinositide hydrolysis in human neutrophils.

  1990cites this paper
• Inhibition of BC3H-1 cell growth by heparin is related to decreased mitogenic signalling.

  1990cites this paper
• Immunoaffinity purification and immunoassay determination of human erythrocyte acylphosphatase

  1990cites this paper
• Permeabilizing parietal cells.

  1990cites this paper
• EGF Triggers a Similar Signalling Cascade in Different Cell Types Overexpressing the EGF Receptor

  1990cites this paper
• Effect of heparin on proliferation and signalling in BC3H‐1 muscle cells

  1990cites this paper
• Thiolprotease inhibitor, EST, can inhibit thrombin-induced platelet activation.

  1990cites this paper
• Regulation of endothelial tissue plasminogen activator and plasminogen activator inhibitor type 1 synthesis by diacylglycerol, phorbol ester, and thrombin

  1990cites this paper
• Effect of antidepressants and neuroleptics on phosphoinositide metabolism in human platelets.

  1990cites this paper
• Signal transduction in EJ‐H‐ras‐transformed cells: De novo synthesis of diacylglycerol and subversion of agonist‐stimulated inositol lipid metabolism

  1989cites this paper
• The role of endogenously formed diacylglycerol in the propagation and termination of platelet activation. A biochemical and functional analysis using the novel diacylglycerol kinase inhibitor, R 59 949.

  1989cites this paper
• Molecular mechanisms of platelet activation.

  1989cites this paper
• Role of calcium on protein phosphorylation in collagen-stimulated platelets. Effect of a new dihydropyridine derivative.

  1989cites this paper
• Regulation of membrane fusion during exocytosis.

  1989cites this paper
• Thrombin and trypsin act at the same site to stimulate phosphoinositide hydrolysis and calcium mobilization.

  1989cites this paper
• Independent expression of human alpha or beta platelet-derived growth factor receptor cDNAs in a naive hematopoietic cell leads to functional coupling with mitogenic and chemotactic signaling pathways.

  1989cites this paper
• Phosphorylation-dependent and -independent pathways of platelet aggregation.

  1989cites this paper
• NIH3T3 fibroblasts transformed by the dbl oncogene show altered expression of bradykinin receptors: effect on inositol lipid turnover.

  1989cites this paper
• Activation of the Inositol-1,4,5-Trisphosphate Signaling System by Acute Ethanol Treatment of Rat Hepatocytes

  1989cites this paper
• Morphine withdrawal in vitro: potentiation of agonist-dependent polyphosphoinositide breakdown.

  1988cites this paper
• The effect of platelet-activating factor on histamine and muscarinic receptor function in guinea pig airways.

  1988cites this paper
• Impairment of Phosphatidylinositol Metabolism in a Patient with a Bleeding Disorder Associated with Defects of Initial Platelet Responses

  1988cites this paper
• Generation and Analysis of a T‐Lymphocyte Somatic Mutant for Studying Molecular Aspects of Signal Transduction by the Antigen Receptor a

  1988cites this paper
• Membrane bilayer balance and platelet shape: morphological and biochemical responses to amphipathic compounds.

  1988cites this paper
• Amiloride analogs stimulate protein phosphorylation and serotonin release in human platelets.

  1988cites this paper
• Tachykinin-induced phosphoinositide breakdown in airway smooth muscle and epithelium: relationship to contraction.

  1988cites this paper
• Amylase secretion from saponin-permeabilized parotid cells evoked by cyclic AMP.

  1988cites this paper
• Effect of exogenously added acylphosphatases on inositol lipid metabolism in human platelets

  1988cites this paper
• Inhibition of adenylate cyclase activity during the reversible shape change in human platelets.

  1988cites this paper
• An immunoreactive form of erythrocyte protein 4.9 is present in non-erythroid cells.

  1988cites this paper
• Alcohol-induced stimulation of phospholipase C in human platelets requires G-protein activation.

  1988cites this paper
• Paradoxical effects of amiloride analogs on protein phosphorylation and serotonin release induced by agonists in human platelets.

  1988cites this paper
• Effect of thrombin inhibitors on thrombin-induced platelet release and aggregation.

  1988cites this paper
• Study on the cupric phenanthroline-induced serotonin release in digitonin-permeabilized platelets.

  1988cites this paper
• The action of the protein kinase C inhibitor, staurosporine, on human platelets. Evidence against a regulatory role for protein kinase C in the formation of inositol trisphosphate by thrombin.

  1988cites this paper
• GABA-receptor stimulation enhances norepinephrine-induced polyphosphoinositide metabolism in rat hippocampal slices.

  1987cites this paper
• Phospholipase C activation induced by noradrenaline in rat hippocampal slices is potentiated by GABA‐receptor stimulation.

  1987cites this paper
• Hormone-regulated phosphoinositide turnover in permeabilized cells and membranes.

  1987cites this paper
• Complement C5b-9-stimulated platelet secretion is associated with a Ca2+-initiated activation of cellular protein kinases.

  1987cites this paper
• Serotonin-induced alterations in inositol phospholipid metabolism in human platelets.

  1987cites this paper
• Polyphosphoinositide metabolism is rapidly stimulated by activation of a temperature-sensitive mutant of Rous sarcoma virus in rat fibroblasts.

  1987cites this paper
• A ROLE OF DIACYLGLYCEROL KINASE IN STIMULUS-SECRET I ON COUPLING OF HUMAN PLATELETS -DISSOCIATION OF SEROTONIN SECRETION FROM Ca2+ MOBILIZATION-

  1987cites this paper
• Effects of Escherichia coli lipopolysaccharide on the phosphoinositide metabolism and serotonin secretion in thrombin-activated platelets.

  1987cites this paper
• Phorbol 12,13-dibutyrate and 1-oleyl-2-acetyldiacylglycerol stimulate inositol trisphosphate dephosphorylation in human platelets.

  1986cites this paper
• Dissociation of Ca2+ mobilization from breakdown of phosphatidylinositol 4,5-bisphosphate in activated human platelets.

  1986cites this paper
• Inositol 1,4,5-trisphosphate induces aggregation and release of 5-hydroxytryptamine from saponin-permeabilized human platelets.

  1986cites this paper
• Characterization of histamine release in digitonin-permeabilized rabbit platelets.

  1986cites this paper
• Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates.

  1986cites this paper
• Sustained proteolysis is required for human platelet activation by thrombin.

  1986cites this paper
• Ionophore A23187 stimulates phosphorylation of the 40,000 dalton protein in human platelets without phospholipase C activation.

  1986cites this paper
• Inositol phospholipid metabolism and platelet function.

  1986cites this paper
• Point-mutated p21ras couples a muscarinic receptor to calcium channels and polyphosphoinositide hydrolysis.

  1986cites this paper
• Reversible shape change in platelets is regulated by phosphatidylinositol metabolism.

  1986cites this paper
• Treatment of human platelets with trypsin, thrombin, or collagen inhibits the pertussis toxin-induced ADP-ribosylation of a 41-kDa protein.

  1986cites this paper
• Differential effects of chlorpromazine on secretion, protein phosphorylation and phosphoinositide metabolism in stimulated platelets.

  1986cites this paper
• Effect of pertussis toxin on the phosphodiesteratic cleavage of the polyphosphoinositides by guanosine 5'-O-thiotriphosphate and thrombin in permeabilized human platelets.

  1986cites this paper
• Leupeptin selectively inhibits human platelet responses induced by thrombin and trypsin; a role for proteolytic activation of phospholipase C.

  1985cites this paper
• Inhibitory action of phorbol myristate acetate on histamine secretion and polyphosphoinositide turnover induced by compound 48/80 in mast cells.

  1985cites this paper
• A role for inositol triphosphate in intracellular Ca2+ mobilization and granule secretion in platelets.

  1985cites this paper
• ATP depletion in human platelets caused by permeabilization with saponin does not prevent serotonin secretion induced by collagen.

  1985cites this paper