Hepatitis B Virus S Protein Enhances Sperm Apoptosis and Reduces Sperm Fertilizing Capacity In Vitro

Objective Studying the impact of Hepatitis B virus S protein (HBs) on early apoptotic events in human spermatozoa and sperm fertilizing capacity. Methodology/Principal Findings Spermatozoa were exposed to HBs (0, 25, 50, 100 µg/ml) for 3 h, and then fluo-4 AM calcium assay, Calcein/Co2+ assay, protein extraction and ELISA, ADP/ATP ratio assay, sperm motility and hyperactivation and sperm-zona pellucida (ZP) binding and ZP-induced acrosome reaction (ZPIAR) tests were performed. The results showed that in the spermatozoa, with increasing concentration of HBs, (1) average cytosolic free Ca2+ concentration ([Ca2+]i) rose; (2) fluorescence intensity of Cal-AM declined; (3) average levels of cytochrome c decreased in mitochondrial fraction and increased in cytosolic fraction; (4) ADP/ATP ratios rose; (5) average rates of total motility and mean hyperactivation declined; (6) average rate of ZPIAR declined. In the above groups the effects of HBs exhibited dose dependency. However, there was no significant difference in the number of sperms bound to ZP between the control and all test groups. Conclusion HBs could induce early events in the apoptotic cascade in human spermatozoa, such as elevation of [Ca2+]i, opening of mitochondrial permeability transition pore (MPTP), release of cytochrome c (cyt c) and increase of ADP/ATP ratio, but exerted a negative impact on sperm fertilizing capacity.

• Publication year

  2013

• Venue

  PLoS ONE

• Publication date

  2013-07-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0068688PMID 23874723PMCID 3712947
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2010cited by this paper
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  2009cited by this paper
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  2007cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2001cited by this paper
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  2000cited by this paper
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  2000cited by this paper
• The mitochondrial permeability transition pore and its role in cell death.

  1999cited by this paper
• Caspases: enemies within.

  1998cited by this paper
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  1997cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1995cited by this paper
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  1993cited by this paper
• [Distribution of hepatitis B virus in testicle tissue in patients with hepatitis B infection].

  1993cited by this paper
• Effects of cryopreservation on human sperm acrosomes.

  1991cited by this paper
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  1988cited by this paper
• Acrosome reaction in human spermatozoa.

  1986cited by this paper
• Presence of HBV DNA in spermatozoa: A possible vertical transmission of HBV via the germ line

  1985cited by this paper
• Experimental transmission of hepatitis B virus by semen and saliva.

  1980cited by this paper
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  1964cited by this paper
• Physiology of reproduction.

  1948cited by this paper

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  2020cites this paper
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  2020cites this paper
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